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Oligonucleotide Fingerprinting in Plants and Fungi

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DNA Fingerprinting: Approaches and Applications

Part of the book series: Experientia Supplementum ((EXS,volume 58))

Summary

Synthetic oligonucleotides complementary to simple repetitive DNA sequence motifs are now routinely applied for multilocus DNA fingerprinting of humans and a large variety of animal species. Most recently, these probes have also been used successfully for the analysis of plant and fungal genomes. All simple motifs investigated to date (CA-, CT-, GATA-, GACA-, GAA-, GTG - GGAT- and TCC-multimers) are present and repeated to various extents throughout the plant and fungal kingdoms. Usually, these probes reveal intra- and interspecific genetic variability resulting in polymorphic or even hypervariable banding patterns. Depending on the combination of species and oligonucleotide probe, species- variety-, accession-, strain-or individual-specific “fingerprints” were obtained in plants and fungi. Somatic stability was observed. For their successful application to DNA fingerprinting, the optimal probe/species-combinations that give distinct banding patterns have to be developed empirically. Various applications of plant DNA fingerprinting using oligonucleotide probes are suggested: (1) characterization of the extent of genetic variability within races, (2) assessment of the “purity” of inbred lines, (3) selection of the recurrent parental genome in backcross breeding programs, (4) identification of crop cultivars and fungal strains, (5) characterization of fusion hybrids, (6) evaluation of the extent of somaclonal variation at the molecular level.

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Authors and Affiliations

  1. Pflanzliche Molekularbiologie, Fachbereich Biologie, Johann Wolfgang Goethe-Universität, Frankfurt, Germany

    K. Weising, J. Ramser, D. Kaemmer & G. Kahl

  2. Max-Planck-Institut für Psychiatrie, Martinsried, Germany

    J. T. Epplen

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  1. K. Weising
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  2. J. Ramser
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  3. D. Kaemmer
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  4. G. Kahl
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  5. J. T. Epplen
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Editors and Affiliations

  1. Dept. Zoology, University of Leicester, LE1 7RH, Leicester, England

    Terry Burke

  2. Institut für Tierzucht, Universität Bern, 3012, Bern, Switzerland

    Gaudenz Dolf

  3. Dept. Genetics, University of Leicester, LE1 7RH, Leicester, England

    Alec J. Jeffreys

  4. UCSF-HSE 1556, Box # 0554, 94143-0554, San Francisco, CA, USA

    Roger Wolff

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© 1991 Birkhäuser Verlag Basel/Switzerland

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Weising, K., Ramser, J., Kaemmer, D., Kahl, G., Epplen, J.T. (1991). Oligonucleotide Fingerprinting in Plants and Fungi. In: Burke, T., Dolf, G., Jeffreys, A.J., Wolff, R. (eds) DNA Fingerprinting: Approaches and Applications. Experientia Supplementum, vol 58. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7312-3_22

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  • DOI: https://doi.org/10.1007/978-3-0348-7312-3_22

  • Publisher Name: Birkhäuser Basel

  • Print ISBN: 978-3-0348-7314-7

  • Online ISBN: 978-3-0348-7312-3

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