Summary
Polyclonal antibodies were raised by the repeated injection of rabbits with a synthetic peptide corresponding to a highly selective portion (amino-acid residues 243 to 268) of the amino-acid sequence of the rat 5-HT1A receptor. The anti-peptide antiserum allowed the immunoprecipitation of 5-HT1A receptors but not of other 5-HT receptor types and adrenergic receptors solubilized from rat hippocampal membranes. Immuno-autoradiographic labelling of rat brain sections with the anti-peptide antiserum exhibited the same regional distribution as 5-HT1A sites labelled by selective radioligands such as [3H]8-OH-DPAT and [125I] BH-8-MeO-N-PAT. However, regional differences apparently existed between the respective intensity of labelling by the agonist radioligands and the antiserum, which might be explained by variations in the degree of coupling of 5-HT1A receptor binding subunits with G proteins from one brain area to another.
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© 1991 Birkhäuser Verlag Basel/Switzerland
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Hamon, M., El Mestikawy, S., Riad, M., Gozlan, H., Daval, G., Vergé, D. (1991). Specific Antibodies as New Tools for Studies of Central 5-HT1AReceptors. In: Fozard, J.R., Saxena, P.R. (eds) Serotonin: Molecular Biology, Receptors and Functional Effects. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7259-1_9
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