Summary
The effect of various inhibitors on the activity of protein kinase C (PKC) was investigated. Studies in vitro with purified rat brain PKC were carried out alongside studies using tumor and normal tissue cell lines. Suramin, an anti-cancer drug, was found to inhibit PKC activity and induce differentiation in neuroblastoma cell clone NB2A. Staurosporine, a potent inhibitor of PKC activity, also inhibited the phorbol ester induced activation of the Na+/H+ antiporter in smooth muscle cells. However once the antiporter had been activated, inhibitors of PKC were not effective supporting a mode in which the Na+/H+ antiporter conserves memory of its activation.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Referencests
Berk, B.C., Aronow, M.S., Brock, T.A., Cragoe, E., Gimbrone, M.A., and Alexander, R.W. (1987) J. Biol. Chem. 262, 5057–5064.
Besterman, J.M., and Cuatrecasas, P. (1984) J. Cell. Biol. 99, 340–343.
Cassel, D., Katz, M., and Rotman, M. (1986) J. Biol. Chem. 261, 5460–5466.
Castagna, M., Takai, Y., Kaibuchi, K., Sano, K., Kikkawa, U., and Nishizuka, Y. (1982) J. Biol. Chem. 257, 7847–7851.
Coffey, R., Leof, E., and Shipley, G. (1987) J. Cell. Phys. 132, 143–148.
Collins, J.M., Klecker, R.W., Yarchoan, R., Lane, H.C., Fauci, A.S., Redfield, R.R., Broder, S.B., and Myers, C.E. (1986) J. Clin. Pharmacol. 26, 22–26.
Coussens, L., Parker, P.J., Rhee, L., Yang Feng, T.L., Chen, E., Waterfield, M.D., Francke, U., and Ullrich, A. (1986) Science 233, 859–866.
Fortes, P.A., Ellory, J.C., and Lew, V.L. (1973) Biochim. Biophys. Acta. 318, 262–272.
Froscio, M., Solanki, V., Murray, A.W., and Hurst, N.P. (1988) Biochem. Pharmacol. 37, 366–368.
Grinstein, S., Cohen, S., Goetz, J.D., Rothstein, A., and Gelfand, E.W. (1985) Proc. Natl. Acad. Sci U.S.A. 82, 1429–1433.
Grinstein, S., and Rothstein, A. (1986) J. Membr. Biol. 90, 112.
Grinstein, S., Rotin, D., and Mason, M.J. (1989) Biochim. Biophys. Acta 988, 73–97.
Hart, D., Langridge, A., Barlow, D., and Sutton, B. (1989) Parasitology Today 5, 117–120.
Hatori, N., Fine, B.P., Nakamura, A., Cragoe, E.Jr., and Aviv, A. (1987) J. Biol. Chem. 262, 5073–5078.
Huang, F.L., Yoshida, Y., Nakabayashi, H., and Huang, K.P. (1987) J. Biol. Chem. 262, 15714–15720.
Inoue, M., Kishimoto A., Takai, Y., and Nishizuka, Y. (1977) J. Biol. Chem. 252, 7610–7616.
Ives, H.E., and Daniel, T.O. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 1950–1954.
Layton, D., and Azzi, A. (1974) Biochem. Biophys. Res. Commun. 59, 322–325.
Liles, W.C., Hunter, D.D., Meier, K.E., and Nathanson, N.M. (1986) J. Biol. Chem. 261, 5307–5313.
Little, P.J., Weissberg, L., Cragoe, E.J., Jr., and Bobik, A. (1988) J.Biol.Chem. 263, 16780–16786.
Magnaldo, I., L’Allemain, G., Chambard, J.C., Moenner, M., Barritault, D., and Pouyssegur, J. (1986) J. Biol. Chem. 261, 16916–16922.
Mahoney, C.W., Azzi, A. (1988) Biochem. Biophys. Res. Commun. 154, 694–697.
Mahoney, C.W., Hensey, C.E., Azzi, A. (1989) Biochemical Pharmacology 38, 3383–3386.
Mahoney, C.W., and Azzi, A. (1989) In: Molecular Biology of Human Diseases, Vol 1 (J.W. Gorrod, O. Albano and S. Papa, Eds), Ellis Horwood Ltd, England, pp. 33–46.
Misset, O., and Opperdoes, F.R. (1987) Eur. J. Biochem. 162, 493–500.
Moolenaar, W.H., Tertoolen, L.G., and de Laat, S.W. (1984) Nature 312, 371–374.
Moolenaar, W.H. (1988) Annu. Rev. Physiol. 48, 363–376. Nishizuka, Y. (1984) Nature 308, 693–698.
Nishizuka, Y. (1986) Science 233, 305–312.
Nishizuka, Y. (1988) Nature 334, 661–665.
Ohno, S., Kawasaki, H., Imajoh, S., Suzuki, K., Inagaki, M., Yokokura, H., Sakoh, T., and Hidaka, H. (1987) Nature 325, 161–166.
Omura, S., Iwai, Y., Hirano, A., Nakagawa,A., Awaya, J., Tsuchiya, H., Takahashi, Y., and Masuma, R. (1977) J. Antibiot. 37, 275–282.
Owen, N.E., Knapik, J., Strebel, F., Tarpley, W.G., and Gorman, R.R. (1989) Am. J. Physiol. 256, C756 - C763.
Parker, P.J., Coussens, L., Totty, N., Rhee, L., Young, S., Chen, E., and Stabel, S. (1986) Science 233, 853–859.
Rozengurt, E. (1986) Science 234, 161–166.
Sharkey, N.A., Leach, K.L., and Blumberg, P.M. (1984) Proc. Natl. Acad. Sci. U.S.A. 81, 607–610.
Sharkey, N.A., and Blumberg, P.M. (1985) Biochem. Biophys. Res. Commun. 133, 1051–1056.
Stein, C.A., LaRocca, R.V., McAtee, T.N., and Myers, C.E. (1989) J. Clin. Oncol. 7, 499–508.
Takai,.Y., Kishimoto, A., Inoue, M., and Nishizuka, Y. (1977) J. Biol. Chem. 252, 7603–7609.
Takai, Y., Kishimoto, A., Kikkawa, U., Mori, T., and Nishizuka, Y. (1979) Biochem. Biophys. Res. Commun. 91, 1218–1224.
Tamaoki, T., Nomoto, H., Takahashi, I., Kato, Y., Morimoto, M., and Tornita, F. (1986) Biochem. Biophys. Res. Commun. 135, 397–402.
Vigne, P., Frelin, C., and Lazdunski, M. (1985) J.Biol.Chem. 260, 8008–8013.
Walter, R.D. (1980) Molec. Biochem. Parasitol. 1, 139–142. Witters, L.A., Vater, C.A., and Lienhard, G. E. (1985) Nature 315, 777–778.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Birkhäuser Verlag Basel
About this chapter
Cite this chapter
Hensey, C.E., Boscoboinik, D., Azzi, A. (1990). Control of Protein Kinase C Function: An Inhibitor Study. In: Vasilescu, D., Jaz, J., Packer, L., Pullman, B. (eds) Water and Ions in Biomolecular Systems. Advances in Life Sciences. Birkhäuser Basel. https://doi.org/10.1007/978-3-0348-7253-9_10
Download citation
DOI: https://doi.org/10.1007/978-3-0348-7253-9_10
Publisher Name: Birkhäuser Basel
Print ISBN: 978-3-0348-7255-3
Online ISBN: 978-3-0348-7253-9
eBook Packages: Springer Book Archive