Abstract
The mouse MT-1 gene, cloned into the plasmid pBR 322, has been injected into fertilised eggs of the rainbow trout, Salmo gairdneri. About 106 copies of the construct, either circular or linearized, have been injected into 1 cell stage eggs within 4 hours of fertilisation, and fry hatched from such eggs are being assayed for evidence of integration of the injected sequence. In addition, with a view to the development of an assay which can be used to monitor for expression of the injected gene, uninjected fry have been exposed to various concentrations of cadmium chloride or zinc sulphate, with or without prior exposure to inducing doses of these compounds. Whereas a distinct protection is evident following induction with zinc, no such protection has been detected with cadmium induction.
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References
Palmiter, R.D. Brinster, R.L., Hammer, R.E., Trumbauer, M.E., Rosenfeld, M.G., Birnberg, N.C. and Evans, R.M. (1982). Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion gene. Nature 300, 611–615.
Spradling, A.C., and Rubin, G.M. (1983). The effect of chromosomal position on the expression of the Drosophila Xanthine Dehydrogenase gene. Cell, 34, 47–57.
Thomas, D.G., Brown, M.W., Shurben, D., Solbe, J.F. del G., Cryer, A., Kay, J. (1985). The sequestration of cadmium and zinc in the tissues of rainbow trout following exposure to the metals singly or in combination. Comp. Biochem. Physiol. 82C, 55–62.
Kay, J., Brown, M.W., Cryer, A., Solbe, J.F. del G., Shurben, D., Garvey, J.S. and Thomas, D.G. (1985) in Metallothionein and other low molecular weight metal binding proteins (Kagi, J.H.R. ed.) Birmhauser Verlag, Basle.
Koropatnick, J., Winning, R., Wiese, E., Heschl, M., Gedama, L., and Duerksen, J. (1985). Acute treatment of mice with cadmium salts results in amplification of the metallothionein-I gene in liver. Nuc. Acids Res. 13 5423–39.
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© 1987 Springer Basel AG
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Maclean, N., Woodall, C., Crossley, F. (1987). Injection of the Mouse MT-1 Gene into Rainbow Trout Eggs and Assay of Trout Fry for Resistance to Cadmium and Zinc Toxicity. In: Kägi, J.H.R., Kojima, Y. (eds) Metallothionein II. Experientia Supplementum, vol 52. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6784-9_47
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DOI: https://doi.org/10.1007/978-3-0348-6784-9_47
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-0348-6786-3
Online ISBN: 978-3-0348-6784-9
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