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Metallothionein-Determination in Biological Materials: Interlaboratory Comparison of 5 Current Methods

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Book cover Metallothionein II

Part of the book series: Experientia Supplementum ((EXS,volume 52))

Abstract

Five current methods of metallothionein (MT)-estimation in biological materials (Hg/TCA-, Cd/heme-, SH-assay, G 75-method and RIA) were compared. Uniform calibration was ensured by measuring the nitrogen-content of the standard-apoprotein after “Kjeldahl-digestion”. Known amounts of rabbit standard Cd-MT I were recovered from identical rat S9-samples. In an overall rating, RIA and Cd/heme-assay performed best, whereas the other methods either underestimated (SH-, G 75) or overestimated (Hg/TCA) the 100%-expectation.

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Author information

Authors and Affiliations

  1. Institut für Toxikologie, Moorenstrasse 5, D-4000, Düsseldorf 1, Germany

    Dr. H. H. Dieter & L. Müller

  2. Institute of Environmental Hygiene, Düsseldorf, Germany

    J. Abell

  3. Institute of Toxicology and Biochemistry, GSF Research Center, 8042, Neuherberg, Germany

    K. H. Summer

Authors
  1. Dr. H. H. Dieter
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  2. L. Müller
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  3. J. Abell
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  4. K. H. Summer
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Editor information

Editors and Affiliations

  1. Institute of Biochemistry, University of Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland

    Jeremias H. R. Kägi

  2. Dept. of Environmental Medicine, Hokkaido University, Kita-ku, Sapporo, 060, Japan

    Yutaka Kojima

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© 1987 Springer Basel AG

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Dieter, H.H., Müller, L., Abell, J., Summer, K.H. (1987). Metallothionein-Determination in Biological Materials: Interlaboratory Comparison of 5 Current Methods. In: Kägi, J.H.R., Kojima, Y. (eds) Metallothionein II. Experientia Supplementum, vol 52. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6784-9_33

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  • DOI: https://doi.org/10.1007/978-3-0348-6784-9_33

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-6786-3

  • Online ISBN: 978-3-0348-6784-9

  • eBook Packages: Springer Book Archive

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