Abstract
It has been observed from 10 years, in America as in Europe, a severe outbreak of Dutch elm disease (Ulmus sp.) caused by Ceratocystis Ulmi. When cultured in liquid medium, the fungus release toxins (1). On the other hand, it has been shown previously, that protoplasts are useful tools to select plants for resistance (2) (3). Such technic presents much more interest with woody plants as the usual methods of genetic improvement are very long. Therefore, isolation and culture of leaf protoplasts were tried from several Ulmus species.
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Dorion, N., Godin, B., Bigot, C. (1983). Isolation and Culture of Leaf Protoplasts from Ulmus sp.: Preliminary Report. In: Potrykus, I., Harms, C.T., Hinnen, A., Hütter, R., King, P.J., Shillito, R.D. (eds) Protoplasts 1983. EXS 45: Experientia Supplementum, vol 45. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6556-2_4
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DOI: https://doi.org/10.1007/978-3-0348-6556-2_4
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