Abstract
It may be possible to improve Trifolium repens (white clover) by somatic hybridisation with other forage legumes. (1) An essential prerequisite for this type of genetic manipulation is the isolation of protoplasts able to divide and reliably regenerate into whole plants with high efficiency. Leaf mesophyll cells are a convenient source of protoplasts and plants have been regenerated from leaf mesophyll protoplasts of the forage legumes Medicago sativa, (2) M. coerulea, M. glutinosa (3) and Trigonella corniculata. (4).
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© 1983 Springer Basel AG
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White, D.W.R. (1983). Plant Regeneration from Mesophyll Protoplasts of White Clover (Trifolium repens L.). In: Potrykus, I., Harms, C.T., Hinnen, A., Hütter, R., King, P.J., Shillito, R.D. (eds) Protoplasts 1983. EXS 45: Experientia Supplementum, vol 45. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6556-2_28
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DOI: https://doi.org/10.1007/978-3-0348-6556-2_28
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