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Lycopersicon Esculentum: Globular Embryos from Microspores and Calli from Diploid Protoplasts

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Protoplasts 1983

Part of the book series: EXS 45: Experientia Supplementum ((EXS,volume 45))

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Abstract

The production of haploid plants via anther culture and plant regeneration from either haploid or diploid protoplasts has been described for many genera within the Solanaceae. In the case of the cultivated tomato (Lycopersicon esculentum) methods for the recovery of plants from mesophyll protoplasts and from protoplasts of long-term cell cultures have been previously published in the cvs. Nadja and Lukullus, respectively (1,2). However, successful induction of androgenesis in the cultivated tomato has only been reported once and has not been repeated so far (3). The goal of our work was

  • to develop culture conditions which allow subsequent division of isolated tomato microspores

  • to built up a protoplast regeneration system in order to extend the list of tomato cultivars, from which protoplast-derived calli or plants can be obtained.

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References

  1. Koblitz, H. and Koblitz, D. (1982) Plant Cell Reports 1, 143–146 and 147–150.

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  2. Morgan, A. and Cocking, E.C. (1982) Z. Pflanzenphys. 106, 97–104.

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  3. Gresshoff, P.M. and Doy, C.H. (1972) Planta 107, 161–170.

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  4. Imamura, J., Okabe, E., Masaharu, K. and Harada, H. (1982) Plant and Cell Physiol. 23, 713–716.

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  5. Nitsch, C. (1977) in: Reinert, J. and Bajaj, Y.P.S. (eds.) Appl. Fund. Asp. Plant Cell, Tissue and Organ Culture, p. 268–278.

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Author information

Authors and Affiliations

  1. Friedrich Miescher-Institut, P.O. Box 2543, CH - 4002, Basel, Switzerland

    S. Krueger-Lebus, I. Potrykus & J. Imamura

Authors
  1. S. Krueger-Lebus
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  2. I. Potrykus
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  3. J. Imamura
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Editor information

Editors and Affiliations

  1. Basel, Switzerland

    I. Potrykus , A. Hinnen , P. J. King  & R. D. Shillito , ,  & 

  2. ZĂĽrich, Switzerland

    C. T. Harms  & R. Hütter  & 

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© 1983 Springer Basel AG

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Krueger-Lebus, S., Potrykus, I., Imamura, J. (1983). Lycopersicon Esculentum: Globular Embryos from Microspores and Calli from Diploid Protoplasts. In: Potrykus, I., Harms, C.T., Hinnen, A., Hütter, R., King, P.J., Shillito, R.D. (eds) Protoplasts 1983. EXS 45: Experientia Supplementum, vol 45. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-6556-2_22

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  • DOI: https://doi.org/10.1007/978-3-0348-6556-2_22

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-6557-9

  • Online ISBN: 978-3-0348-6556-2

  • eBook Packages: Springer Book Archive

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