Summary
The effect of irradiation on human hemoglobin A solutions was investigated by two methods: estimation of redox potential by the method of Behlke and Scheler and recording of the absorption spectra. In the case of the redox reaction, from the titration curves free energy values and then other thermodynamic values may be calculated. A decrease of n value, following irradiation, was observed for the doses in the range of 105 rad. For lower doses, the first change, possible to observe by the methods used, was the gradual passing of OxyHb into MetHb spectrum.
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References
D.L. Drabkin, J. Biol. Chem. 164, 703 (1946).
J. Behlke and W. Scheler, Acta Biol. Med. Germ. 8, 88 (1962).
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E. Staunton West, Textbook of Biophysical Chemistry, The Macmillan Co., New York, 1963.
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© 1971 Springer Basel AG
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Leyko, W., Wiszkowska, H., Puchala, M. (1971). Effect of X and γ-Irradiation on Hemoglobin A Redox System. In: Milazzo, G., Jones, P.E., Rampazzo, L. (eds) Biological Aspects of Electrochemistry. Experientia Supplementum, vol 18. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-5848-9_38
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DOI: https://doi.org/10.1007/978-3-0348-5848-9_38
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-0348-5850-2
Online ISBN: 978-3-0348-5848-9
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