Summary
A new cross-flow reaction chamber has been developed for an automatic sequencer, which allows 1.5% improved repetitive yields due to the dual-phase reaction conditions. The amount of 150 pmol initial yield could be sequenced up to sixty steps. The cross-flow reactor is also well suited to gas-phase or solid-phase techniques, but samples of more than six residues are almost equally well sequenced after application to PVDF membrane or covalent attachment to a suitable support.
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Abbreviations
- BPTI:
-
bovine pancreatic trypsin inhibitor
- MZE:
-
multiphase zone electrophoresis
- PAGE:
-
polyacrylamide gel electrophoresis
- PMN:
-
polymorphonuclear
- PMNL:
-
polymorphonuclear leucocytes
- PTH:
-
phenylthiohydantoin
- PVDF:
-
polyvinylidene difluoride
- SDS:
-
sodium dodecylsulfate
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© 1991 Springer Basel AG
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Reinke, H., Fischer, S., Reimann, F., Tschesche, H. (1991). Direct Microsequencing of Blotted and Covalently Attached Proteins in a Cross-Flow Reaction Chamber. In: Jörnvall, H., Höög, JO., Gustavsson, AM. (eds) Methods in Protein Sequence Analysis. Advances in Life Sciences. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-5678-2_5
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DOI: https://doi.org/10.1007/978-3-0348-5678-2_5
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