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Structural Analysis of the Glucocorticoid Receptor Protein

  • Chapter
Methods in Protein Sequence Analysis

Part of the book series: Advances in Life Sciences ((ALS))

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Summary

The glucocorticoid receptor protein (GR) is a ligand-dependent protein that regulates transcription. The steroid-binding and DNA-binding functions are localised in separate domains. Three steroid-binding amino acid residues within the steroid-binding domain have been identified by affinity-labelling. The DNA-binding domain (DBD) has been expressed in an isolated form and further characterised. The isolated DBD binds to DNA as a dimer in a cooperative manner and with the same specificity as the intact GR. The segment of DBD involved in the cooperativity has been identified. The three-dimensional structure of DBD has been determined by NMR-spectroscopy. Contact sites between DBD and DNA are being investigated by DNA-modification (missing base contact probing and ethylation interference) and site-directed mutagenesis in order to fully understand the mode of interaction.

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Author information

Authors and Affiliations

  1. Department of Medical Nutrition, Centre for Biotechnology and Centre for Nutrition and Toxicology, Karolinska Institutet, Huddinge University Hospital F60, Novum, S-141 86, Huddinge, Sweden

    J. Carlstedt-Duke, P.-E. Strömstedt, K. Dahlman-Wright, T. Härd, J. Zilliacus, C. Cairns, A. Wright, I. McEwan, L. Perez, H. Jörnvall & J.-Å. Gustafsson

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  1. J. Carlstedt-Duke
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  2. P.-E. Strömstedt
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  3. K. Dahlman-Wright
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  4. T. Härd
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  5. J. Zilliacus
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  6. C. Cairns
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  7. A. Wright
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  8. I. McEwan
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  9. L. Perez
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  10. H. Jörnvall
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  11. J.-Å. Gustafsson
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Editor information

Editors and Affiliations

  1. Department of Chemistry I, Karolinska Institute, S-104 01, Stockholm, Sweden

    Hans Jörnvall , Jan-Olov Höög  & Ann-Margreth Gustavsson ,  & 

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© 1991 Springer Basel AG

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Carlstedt-Duke, J. et al. (1991). Structural Analysis of the Glucocorticoid Receptor Protein. In: Jörnvall, H., Höög, JO., Gustavsson, AM. (eds) Methods in Protein Sequence Analysis. Advances in Life Sciences. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-5678-2_30

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  • DOI: https://doi.org/10.1007/978-3-0348-5678-2_30

  • Publisher Name: Birkhäuser, Basel

  • Print ISBN: 978-3-0348-5680-5

  • Online ISBN: 978-3-0348-5678-2

  • eBook Packages: Springer Book Archive

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