Summary
Analysis of amino acid derivatives at the femtomole level is a challenge in the field of protein sequence analysis. 2-Anilino-5-thiazolinone (ATZ-) amino acids obtained by Edman degradation, were quantitatively derivatized with 4-aminofluorescein. The amino acid derivatives sensitized with this fluorescent reagent were separated using reversed-phase HPLC and identified at the 100 attomole level. Incorporation of this method into the operation of a conventional automated sequencer is extensively studied and several modifications are proposed including the use of heptafluorobutyric acid for the cyclization step.
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References
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Tsugita, A., Kamo, M., Jone, C.S., and Shikama, N. (1989) J. Biochem. 106, 60–65.
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© 1991 Springer Basel AG
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Tsugita, A., Kamo, M. (1991). Sensitive Determination of Amino Acid Derivatives from N-Terminal Sequence Analysis. In: Jörnvall, H., Höög, JO., Gustavsson, AM. (eds) Methods in Protein Sequence Analysis. Advances in Life Sciences. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-5678-2_11
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DOI: https://doi.org/10.1007/978-3-0348-5678-2_11
Publisher Name: Birkhäuser, Basel
Print ISBN: 978-3-0348-5680-5
Online ISBN: 978-3-0348-5678-2
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