Abstract
In recent years numerous laboratories have demonstrated that repeated bouts of exercise (training) will cause the junction strength of bone-ligament-bone preparations to increase [14-17, 19, 21-23]. Similar findings have been reported from measurements of isolated tendons [20]. The biochemical explanation for these changes, specifically as it pertains to bone-ligament-bone preparations, is complex because the point of separation occurs within a transition zone involving ligament-nonmineralized fibrocartilage-mineralized fibrocartilage-bone [15]. Since the structure and function of connective tissue is dependent upon the amount, proportions, and interactions between collagen, elastin, non-collagen proteins and glycoaminoglycans [10], we initiated studies on the influence of training on collagen concentration in ligaments to gain a better understanding of the mechanism responsible for the effects of training. Collagen represents approximately 80% of the dried weight of tendons and essentially 14% of the amino acid residues present in collagen are hydroxyproline [6, 13]. Since hydroxyproline is found almost exclusively within collagen, its measurement can be used to depict changes in the concentration of collagen in tissues from experimental animals [8, 12, 13].
1 Supported in part by funds from NIH Grant AM-08893-08.
2 NDEA Fellow in Exercise Physiology.
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References
Anderson, J. J. B., Milon, L. and Crackel, W. C.: Effect of Exercise on Mineral and Organic Bone Turnover in Swine. J. Appl. Physiol. 30, 810–813 (1971).
Bartosova, D., Chvapil, M., Korecky, B., Poupa, O., Rakusan, K., Turek, A. and Vizek, M.: The Growth of the Muscular and Collagenous Parts of the Rat Heart in Various Forms of Cardiomegaly. J. Physiol. (Lond.) 200, 285–295 (1969).
Bergman, M. and Loxley, R.: Two Improved and Simplified Methods for the Spectrophotometric Determination of Hydroxyproline. Anal. Chem. 35, 1961–1965 (1963).
Chvapil, M.: Physiology of Connective Tissue, p. 333 (Butterworths, London 1967).
Elliott, D. H.: Structure and Function of Mammalian Tendon. Biol. Rev. 40, 392–421 (1965).
Elliott, D. H. and Crawford, G. N. C.: The Thickness and Collagen Content of Tendon Relative to the Strength and Cross Sectional Area of Muscle. Proc. Roy. Soc. B. 162, 137–146 (1965).
Harkness, R. D.: Mechanical Properties of Collagenous Tissue. In: Gould, B. S., (Eds) Treatise on Collagen, Vol. 2, Biology of Collagen, pp. 247–310 ( Academic Press, London 1968 ).
Heikkinen, E. and Vuori, I.: Effect sof Physical Activity on the Metabolism of Collagen in Aged Mice. Acta Physiol. Scand. 84, 543–549 (1972).
Holloszy, J. O.: Biochemical Adaptations in Muscle. J. Biol. Chem. 242, 2278–2882 (1967).
Jackson, D. S. and Bentley, J. F.: Collagen-Glycosaminoglycan Interactions. In: Gould, B. S., (Eds) Treatise on Collagen, Vol. 2, Biology of Collagen, pp. 189–214 ( Academic Press, London 1968 ).
Palmer, W. R. and Tipton, C. M.: Influence of Hypophysectomy and Training on Size of Isolated Fat Cells. Am. J. Physiol. 224, 1206–1209 (1973).
Prockop, J. J. and Kivirikko, K. I.: Hydroxyproline and the Metabolism of Collagen. In: Gould, B. S., (Eds) Treatise on Collagen, Vol. 2, Biology of Collagen, pp. 215–246 ( Academic Press, London 1968 ).
Sjoerdsma, A., Udenfriend, S., Keiser, H. and Leroy, C.: Hydroxyproline and Collagen Metabolism. Ann. Int. Med. 63, 672–694 (1965).
Tipton, C. M., Matthes, R. D. and Sandage, D. S.: In situ Measurement of Junction Strength and Ligament Elongation in Rats. J. Appl. Physiol. 37, 758–761 (1974).
Tipton, C. M., Schild, R. J. and Tomanek, R. J.: Influence of Physical Activity on the Strength of Knee Ligaments in Rats. Am. J. Physiol. 212, 783–787 (1967).
Tipton, C. M., James, S. L., Mergner, W. and Tcheng, T. K.: Influence of Exercise on Strength of Medial Collateral Knee Ligaments of Dogs. Am. J. Physiol. 218, 894–902 (1970).
Tipton, C. M., Tcheng, T. K. and Mergner, W.: Ligamentous Strength Measurements from Hypophysectomized Rats. Am. J. Physiol. 221, 1144–1150 (1971).
Tomanek, R. J., Taunton, C. A. and Llsxop, K. S.: Relationship Between Age, Chronic Exercise and Connective Tissue of the Heart. J. Gerontol. 1, 33–38 (1972).
Viidik, A.: Biomechanics and Functional Adaptation of Tendons and Joint Ligaments. In: Evans, F. G., (Eds) Studies on the Anatomy and Function of Bones and Joints, pp. 17–39 ( Springer, Berlin 1965 ).
Viidik, A.: The Effect of Training on the Tensile Strength of Isolated Rabbit Tendons. Scand. J. Plast. Reconstr. Surg. 1, 141–147 (1967).
Viidik, A.: Elasticity and Tensile Strength of the Anterior Cruciate Ligament in Rabbits as Influenced by Training. Acta Physiol. Scand. 74, 372–380 (1968).
Zuckerman, J. and Stull, G. A.: Effects of Exercise on Knee Ligament Separation Force in Rats. J. Appl. Physiol. 26, 716–719 (1969).
Zuckerman, J. and Stull, G. A.: Ligamentous Separation Force in Rats as Influenced by Training, Detraining, and Cage Restriction. Med. Sci. Sports. 5, 44–49 (1973).
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Tipton, C.M., Martin, R.K., Matthes, R.D., Carey, R.A. (1975). Hydroxyproline Concentrations in Ligaments From Trained and Nontrained Rats. In: Howald, H., Poortmans, J.R. (eds) Metabolic Adaptation to Prolonged Physical Exercise. Birkhäuser, Basel. https://doi.org/10.1007/978-3-0348-5523-5_30
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