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Rapid Reaction Kinetic Techniques

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Fluorescent Methods for Molecular Motors

Part of the book series: Experientia Supplementum ((EXS,volume 105))

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Abstract

Most biochemical processes occur on sub-second time scales. Relaxation and rapid mixing methods allow reactions from microsecond time scales onwards to be monitored in real time. This chapter describes the instrumentation for these techniques and it discusses general topics of sample excitation and signal detection.

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Abbreviations

CCD:

Charge-coupled device

Deac:

Diethylaminocoumarin

DWR:

Direct wave recording

FRET:

Förster (or fluorescence) resonance energy transfer

k cat :

Catalytic turnover number

K d :

Equilibrium dissociation constant

K m :

Michaelis constant

k obs :

Observed rate constant

LED:

Light-emitting diode

Mant:

2′(3′)-O-(N-Methylanthraniloyl)-

NATA:

N-Acetyl tryptophanamide

Pi :

Inorganic phosphate

PMT:

Photomultiplier tube

λ ex :

Excitation wavelength

References

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Author information

Authors and Affiliations

  1. Chromosome Organisation and Dynamics, Max-Planck Institute of Biochemistry, Martinsried, 82152, Germany

    Christopher P. Toseland

  2. School of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK

    Michael A. Geeves

Authors
  1. Christopher P. Toseland
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  2. Michael A. Geeves
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Corresponding author

Correspondence to Christopher P. Toseland .

Editor information

Editors and Affiliations

  1. Chromosome Organisation and Dynamics, Max-Planck Institute of Biochemistry, Martinsried, Germany

    Christopher P. Toseland

  2. Department of Cellular Physiology, Ludwig-Maximilians-Universität München, Munich, Germany

    Natalia Fili

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Toseland, C.P., Geeves, M.A. (2014). Rapid Reaction Kinetic Techniques. In: Toseland, C., Fili, N. (eds) Fluorescent Methods for Molecular Motors. Experientia Supplementum, vol 105. Springer, Basel. https://doi.org/10.1007/978-3-0348-0856-9_3

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