Abstract
For approximately five decades, immunoassays have been the method of choice for quantifying low concentrations of ANALYTES in complex biological fluids. The procedure is equally applicable to the measurement of small molecules such as drugs and large molecules such as proteins. The technique combines SENSITIVITY and SPECIFICITY with ease of use.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Horton JK, Capper SJ, Price Jones MJ, Hughes KT. Assays for drug screening applications and research. In: Wild D (ed) (2005) The Immunoassay Handbook. Elsevier, Oxford, 854–884
Yalow RS, Berson SA (1959) Assay of plasma insulin in human subjects by immunologic methods. Nature 184: 1684–1689
Voller A, Bidwell DE, Huldt G, Engvall E (1974) A microplate method of enzyme linked immunosorbent assay and its application to malaria. Bull WHO 51: 209–211
O’Sullivan MJ, Marks V (1981) Methods for the preparation of enzyme-antibody-conjugates for use in enzyme immunoassay. Methods Enzymol 73: 147–166
O’Sullivan MJ, Bridges JW, Marks V (1979) Enzyme immunoassay: a review. Annal Clin Biochem 16: 221– 239
Horton JK, Baxendale PM (1995) Mass measurement of cAMP formation by radioimmunoassay, enzyme immunoassay and scintillation proximity assay. Methods Mol Biol 41: 91–105
Kricka LJ, Wild D (2005) Signal generation and detection systems (excluding homogeneous assays). In: Wild D (ed): The Immunoassay Handbook. Elsevier, Oxford, 192–211
Fowler A, Davies I, Norey C (2000) A multi-modality assay platform for ultra-high throughput screening. Curr Pharm Technol 1: 265–281
Ullman EF (2005) Homogeneous immunoassay. In: Wild D (ed): The Immunoassay Handbook. Elsevier, Oxford, 212–232
Wiese R, Belosludtsev Y, Powdrill T, Thompson P, Hogan M (2001) Simultaneous multianalyte ELISA performed on a microarray platform. Clin Chem 47: 1451–1457
Carson RT, Vignali DAA (1999) Simultaneous quantification of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods 227: 41–52
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Birkhäuser Basel
About this chapter
Cite this chapter
Horton, J.K., O’Sullivan, M. (2011). B2 Immunoassays. In: Nijkamp, F., Parnham, M. (eds) Principles of Immunopharmacology. Birkhäuser Basel. https://doi.org/10.1007/978-3-0346-0136-8_13
Download citation
DOI: https://doi.org/10.1007/978-3-0346-0136-8_13
Published:
Publisher Name: Birkhäuser Basel
Print ISBN: 978-3-0346-0135-1
Online ISBN: 978-3-0346-0136-8
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)