Abstract
The research presents an approach to developing in-house reference materials (IHRMs) for a nanosensor system based on dynamic light scattering and fluorescence for qualitative and quantitative determination of the food contaminant antibiotic chloramphenicol (laevomycetin) and reduced glutathione, a marker for ischemic stroke and several other diseases. Chloramphenicol and reduced glutathione were chosen as candidate materials. The certification procedure based on the calculation and experimental method of preparation was employed to establish the certified value of the IHRM. During the tests, the metrological characteristics of the reference material were determined. The certified value of the mass fraction of the IHRM for reduced glutathione is 98.5%, and the expanded uncertainty of the certified value with the coverage factor k = 2 is ± 0.3%. The certified value of the mass concentration of the IHRM for chloramphenicol is 10.0 g/dm3, and the expanded uncertainty of the certified value with the coverage factor k = 2 is ± 4.0%. The use of the developed IHRMs demonstrated their applicability for calibration of optical nanosensor systems based on dynamic light scattering and fluorescence. It is assumed that the developed in-house reference materials can be further certified as certified reference materials (CRM) and used for verification, calibration, and graduation of compact detection devices of the “point of care diagnostics” type designed for express tests right on the sampling site.
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Notes
- 1.
Pharmaceutical Secondary Standard; Certified Reference Material Glutathione 70–18-8. https://www.sigmaaldrich.com/catalog/product/sial/phr1359?lang=en®ion=RU.
- 2.
Pharmaceutical Secondary Standard; Certified Reference Material Glutathione 70-18-8. https://www.sigmaaldrich.com/catalog/product/sial/phr1359?lang=en®ion=RU (Accessed 15 April 2021).
- 3.
SPRM 3–2020 State primary reference material of the unit of mass (kilogram). https://fgis.gost.ru/fundmetrology/registry/12/items/1385582.
- 4.
SPRM 216–2018 State primary reference material of the unit of fluid volume in the range of 1.0·10-9 m3 to 1.0 м3. https://fgis.gost.ru/fundmetrology/registry/12/items/397905.
- 5.
Class E1, E2, F1, F2, and M1 weights (number in the state register 36068-07). https://fgis.gost.ru/fundmetrology/registry/4/items/345346.
- 6.
Single- and multi-channel adjustable pipettes (number in the state register 37432-13). https://fgis.gost.ru/fundmetrology/registry/4/items/346979.
- 7.
Microclimate testers (number in the state register 32,014–11). https://fgis.gost.ru/fundmetrology/registry/4/items/340205.
- 8.
Electronic balance (number in the state register 16,313–08). https://fgis.gost.ru/fundmetrology/registry/4/items/315230.
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Acknowledgements
The authors express their gratitude to Alexander Davidovich Levin, Dr. Sci. (Engineering), a Senior Researcher of the Laboratory of Optical Spectral Devices at VNIIOFI, for the performed calculations and assistance with the preparation of the manuscript for publication. All measurements were made using the equipment of VNIIOFI.
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A.A. Yushina: literature review; preparation of technical specification; data collection and processing; experimental data analysis.
M.K. Alenichev: concept advancement; data collection and processing; preparation of documents for reference material testing for the purpose of type approval.
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The article was prepared on the basis of a report presented at the IV International Scientific Conference “Reference Materials in Measurement and Technology” (St. Petersburg, December 1–3, 2020). The article was admitted for publication after the abstract was revised, the article was formalized, and the review procedure was carried out.
The version in the Russian language is published in the journal “Measurement Standards. Reference Materials” 2021;17(1):59–69. https://doi.org/10.20915/2687-0886-2021-17-1-59-69.
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Yushina, A.A., Alenichev, M.K. (2022). Reference Materials for Optical Nanosensor Systems: Reduced Glutathione and Chloramphenicol. In: Medvedevskikh, S.V., Sobina, E.P., Kremleva, O.N., Okrepilov, M.V. (eds) Reference Materials in Measurement and Technology . RMMT 2020. Springer, Cham. https://doi.org/10.1007/978-3-031-06285-8_16
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