Abstract
Dynamic light scattering (DLS) is one of the most commonly used for rapid particle size determination. The advantages of DLS for fast and simple colloid systems (i.e. food, emulsions) and nanoparticles dispersion characterization is limited due to particle shape and possible interactions between the sample and the measuring solution. In this paper, we present the results of monodispersed polystyrene standardized nanobeads, silver and polydispersed submicron-sized fluorinated particles (HFBMA) and silica synthesized by a modified Stöber method. We compared the obtained results in “standard conditions” with non-standard situations, such as using tap water or employing Ringer solution as a dispersant. Furthermore, the studies were performed at both room temperature (20 °C) and elevated temperature (37 °C) to simulate various possible applications of nanosystem investigations in the biomedical field. The results show that sample behaviours might not only vary with temperature and dispersant changes, but may also fluctuate during the time of measurement (i.e. aggregation process could be observed). Presented results will open discussion about factors other than dilution and concentrations for observing behavior of the nanoparticles and submicron particles in “real conditions”. Our studies confirmed the hypothesis that more than one particle size determination technique is recommended. Moreover, particle size verification is recommended during long-term storage and when environmental conditions change.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Aguilera, J.M.: Why food microstructure? J. Food Eng. 67(1–2), 3–11 (2005)
Arenas-Guerrero, P., et al.: Determination of the size distribution of non-spherical nanoparticles by electric birefringence-based methods. Sci. Rep. 8, 950 (2018)
Bai, Y.X., Li, Y.F., Yang, Y., Yi, L.X.: Covalent immobilization of triacylglycerol lipase onto functionalized nanoscale SiO2 spheres. Process Biochem. 41, 770–777 (2006)
Bell, N.C., Minelli, C., Tompkins, J., Stevens, M.M., Shard, A.G.: Emerging techniques for submicrometer particle sizing applied to Stöber silica. Langmuir 28, 10860–10872 (2012)
Berne, B.J., Pecora, R.: Dynamic Light Scattering: With Applications to Chemistry, Biology, and Physics. Dover Publications, Mineola (1976)
Bhattacharjee, S.: DLS and zeta potential – what they are and what they are not? J. Controlled Release 235, 337–351 (2016)
Chaudhry, Q., Scotter, M., Blackburn, J., Ross, B., Boxall, A., Castle, L.: Applications and implications of nanotechnologies for the food sector. Food Addit. Contam. Part A 25(3), 241–258 (2008)
Dahneke, B.E.: Measurement of Suspended Particles by Quasi-elastic Light Scattering. Wiley, New York (1983)
Gapinski, J., Jarzębski, M., Buitenhuis, J., Deptula, T., Mazuryk, J., Patkowski, A.: Structure and dimensions of core-shell nanoparticles comparable to the confocal volume studied by means of fluorescence correlation spectroscopy. Langmuir 32(10), 2482–2491 (2016)
Jarzebski, M., et al.: Core–shell fluorinated methacrylate nanoparticles with Rhodamine-B for confocal microscopy and fluorescence correlation spectroscopy applications. J. Fluor. Chem. 183, 92–99 (2016)
Jarzębski, M., et al.: Submicron sized fluorescent silica particles characterization. Nucl. Instrum. Methods Phys. Res., Sect. B 411, 78–84 (2017a)
Jarzębski, M., et al.: Particle tracking analysis in food and hydrocolloids investigations Food Hydrocolloids 68, 90–101 (2017b)
Kaszuba, M., McKnight, D., Connah, M.T., McNeil-Watson, F.K., Nobbmann, U.: Measuring subnanometre sizes using dynamic light scattering. J. Nanopart. Res. 10, 823–829 (2008)
Koenderink, G.H., Sacanna, S., Pathmamanoharan, C., Rasa, M., Philipse, A.P.: Preparation and properties of optically transparent aqueous dispersions of monodisperse fluorinated colloids. Langmuir 17, 6086–6093 (2001)
Maulucci, G., et al.: Particle size distribution in DMPC vesicles solutions undergoing different sonication times. Biophys. J. 88, 3545–3550 (2005)
Rashidi, L., Khosravi-Darani, K.: The applications of nanotechnology in food industry. Crit. Rev. Food Sci. Nutr. 51(8), 723–730 (2011)
Sacanna, S., Koenderink, G.H., Philipse, A.P.: Microemulsion synthesis of fluorinated latex spheres. Langmuir 20, 8398–8400 (2004)
Varga, Z., et al.: Towards traceable size determination of extracellular vesicles. J. Extracellular Vesicles 3(1) (2014)
Verhaegh, N.A.M., van Blaaderen, A.: Dispersions of rhodamine-labeled silica spheres: synthesis, characterization, and fluorescence confocal scanning laser microscopy. Langumir 10, 1427–1438 (1994)
Acknowledgments
Authors’ also acknowledge the financial support provided by the Dr. and Mrs. A. S. Dekaban Foundation for supporting the stay of Maciej Jarzebski at the UBC Food Process Engineering Laboratory, Vancouver, Canada.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Nature Switzerland AG
About this paper
Cite this paper
Jarzębski, M. et al. (2020). Nanoparticles Size Determination by Dynamic Light Scattering in Real (Non-standard) Conditions Regulators - Design, Tests and Applications. In: Ochowiak, M., Woziwodzki, S., Mitkowski, P., Doligalski, M. (eds) Practical Aspects of Chemical Engineering. PAIC 2019. Springer, Cham. https://doi.org/10.1007/978-3-030-39867-5_13
Download citation
DOI: https://doi.org/10.1007/978-3-030-39867-5_13
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-39866-8
Online ISBN: 978-3-030-39867-5
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)