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Isolating Cells from Tissue

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Tissue Engineering

Part of the book series: Learning Materials in Biosciences ((LMB))

Abstract

This chapter will briefly touch on the history of cell theory, followed by an overview of main intracellular components. This is followed by a discussion as to why, depending on their primary function, mature cells differ so much in the shapes and intracellular content. We next focus on the methods to isolate and sort different cell types. Main types of centrifugation and differences between them are then introduced, followed by an overview of how cells can be counted and evaluated for their viability after isolation.

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References and Further Reading

  1. R. Bailey, Different cell types in the body. [Online]. Available: https://www.thoughtco.com/types-of-cells-in-the-body-373388. Accessed 13 Jun 2018

  2. M.-L. Izamis et al., Simple machine perfusion significantly enhances hepatocyte yields of ischemic and fresh rat livers. Cell Med. 4(3), 109–123 (2013)

    Article  Google Scholar 

  3. M.N. Berry, D.S. Friend, High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. J. Cell Biol. 43(3), 506–520 (1969)

    Article  CAS  Google Scholar 

  4. T. Powell, V.W. Twist, A rapid technique for the isolation and purification of adult cardiac muscle cells having respiratory control and a tolerance to calcium. Biochem. Biophys. Res. Commun. 72(1), 327–333 (1976)

    Article  CAS  Google Scholar 

  5. D.G. Leuning, E. Lievers, M.E.J. Reinders, C. van Kooten, M.A. Engelse, T.J. Rabelink, A novel clinical grade isolation method for human kidney perivascular stromal cells. J. Vis. Exp. 126 (2017). https://doi.org/10.3791/55841

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Author information

Authors and Affiliations

  1. Yerevan State University, Yerevan, Armenia

    Anna Simonyan

  2. The George Washington University, Washington, DC, USA

    Narine Sarvazyan

Authors
  1. Anna Simonyan
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  2. Narine Sarvazyan
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Corresponding author

Correspondence to Narine Sarvazyan .

Editor information

Editors and Affiliations

  1. The George Washington University, Washington, DC, USA

    Narine Sarvazyan

Self-Check Questions

Self-Check Questions

  1. Q.4.1.

    It is critical to lower the concentration of ________ions when isolating cells from tissues.

    1. A.

      Sodium

    2. B.

      Calcium

    3. C.

      Potassium

    4. D.

      Magnesium

  1. Q.4.2.

    An enzyme that is NOT commonly used for isolation of cells from tissues is

    1. A.

      Collagenase

    2. B.

      Trypsin

    3. C.

      ATPase

    4. D.

      DNAase

  1. Q.4.3.

    The best way to separate cells based on their density is by using

    1. A.

      Flow cytometry

    2. B.

      Isopycnic centrifugation

    3. C.

      Trypan blue staining

    4. D.

      The Coulter counter

  1. Q.4.4.

    Any of the listed approaches can be used to estimate cell sizes, EXCEPT

    1. A.

      Trypan blue staining

    2. B.

      Flow cytometry

    3. C.

      The Coulter counter

    4. D.

      Differential centrifugation

  1. Q.4.5.

    Correct timing to collect sample is critical when performing ______________ centrifugation.

    1. A.

      Step-density gradient

    2. B.

      Isopycnic

    3. C.

      Differential

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Cite this chapter

Simonyan, A., Sarvazyan, N. (2020). Isolating Cells from Tissue. In: Sarvazyan, N. (eds) Tissue Engineering. Learning Materials in Biosciences. Springer, Cham. https://doi.org/10.1007/978-3-030-39698-5_4

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