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Microbial Identification and Extracellular Polymeric Substances Characterization of Aerobic Granules Developed in Treating Rubber Processing Wastewater

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Valorisation of Agro-industrial Residues – Volume I: Biological Approaches

Abstract

The goal of this study was to investigate the species of the abundance microbial in seed sludge and aerobic granular sludge. Experiments were carried out in a sequencing batch reactor with a working volume of 1.6 L. During the start-up period, the reactor was inoculated with 800 mL of sludge from a municipal sewage treatment plant plus 800 mL of rubber processing wastewater. Further investigation by Illumina high-throughput sequencing was performed to analyze the microbial diversity and phylogenetic structures during the granulation of seed sludge to aerobic granules. A diversity of microorganisms was identified from the seed sludge and aerobic granular sludge. Seed sludge consisted of 96.4% bacteria, 1.7% eukaryote, 1.2% archaea, and 0.7% viruses. Aerobic granular sludge consisted of 97.8% bacteria, 1.0% eukaryote, 0.8% archaea, and 0.4% viruses. As the granulation process succeeded in SBR, distinct differences of the microbial community in the seed sludge and aerobic granular sludge were observed, which suggested that there was high microbial selection pressure during granulation in the system. The most abundance species in seed sludge was Dechloromonas aromatica, while Pseudomonas fluorescens was the most abundance species in aerobic granular sludge.

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Authors and Affiliations

  1. Malaysia-Japan International Institute of Technology, Universiti Teknologi Malaysia, Kuala Lumpur, Malaysia

    Aznah Nor Anuar, Mohd Hakim Ab Halim, Hazlami Fikri Basri & Zaini Ujang

  2. Faculty of Civil Engineering, Department of Environmental Engineering, Universiti Teknologi Malaysia, Skudai, Johor, Malaysia

    Noor Hasyimah Rosman & Inawati Othman

  3. Faculty of Engineering Technology, Department of Civil Engineering Technology, Universiti Tun Hussein Onn, Parit Raja, Johor, Malaysia

    Hasnida Harun

  4. Faculty of Applied Sciences, Department of Biotechnology, Delft University of Technology, Delft, The Netherlands

    Mark van Loosdrecht

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  1. School of Chemical and Energy Eng, Faculty of Engineering, Universiti Teknologi Malaysia, Johor Bahru, Malaysia

    Zainul Akmar Zakaria

  2. Department of Biological Sciences, Nicholls State University, Thibodaux, LA, USA

    Ramaraj Boopathy

  3. PROIMI - CONICET, Tucuman, Argentina

    Julian Rafael Dib

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Anuar, A.N. et al. (2020). Microbial Identification and Extracellular Polymeric Substances Characterization of Aerobic Granules Developed in Treating Rubber Processing Wastewater. In: Zakaria, Z., Boopathy, R., Dib, J. (eds) Valorisation of Agro-industrial Residues – Volume I: Biological Approaches. Applied Environmental Science and Engineering for a Sustainable Future. Springer, Cham. https://doi.org/10.1007/978-3-030-39137-9_13

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