Abstract
The leading role in Bacillus thuringiensis B.t. research in Egypt has been taken by the National Research Centre (NRC). The present book chapter is an account of studies that have been carried out on this pathogen in Egypt and its possible role as a biological control agent. These studies included bioassay of various cultures of B.t. to detect the potent strains versus different insect species. The introduction of agro-industrial byproducts in the fermentation media have been explored for economic commercial production of the pathogen. Attempts were made to develop feeding stimulants and bait formulations aiming to overcome short environmental persistence. Novel approaches including the use of chemical additives with B.t. formulations were adopted to enhance potency against insects through biochemical reactions that occurred in the insect midgut. Reference was given to the mode of action of B.t. and its distribution. The joint action of B.t. varieties and its integration with other microbial and chemical control agents was highlighted. Investigations on the pathogen effect on various development stages of Lepidoptera are given. Pilot scale production of B.t. has been described. Studies dealing with the large scale field application of B.t. are given. Reference has been given to the current status of genetically modified technology (GM) in Egypt.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Frankenhuyzen KV (1993) The challenge of Bacillus thuringiensis. In: Entwistle P, Cory J, Bailey M, Higgs S (eds) Bacillus thuringiensis, an environmental biopesticide: theory and practice, pp 1–35
Berliner E (1911) Uber die Schlaffsucht der Mehlmottenraupe. Z Gesamte Getreidewesen Berlin 3:63–70
Berliner E (1915) Uber die Schlaffsucht der Mehlmottenraupe. Z ang Ent 2:29–56
Weiser J (1986) Impact of Bacillus thuringiensis on applied entomology in Eastern Europe and in the Soviet Union. In: Krieg A, Huger AM (eds) Mitteilungen aus der Biologischen Bundesanstalt für Land – und Forstwirtschaft Berlin-Dahlem, vol 233. Paul Parey, Berlin, pp 37–50
Steinhaus EA (1951) Possible use of Bacillus thuringiensis as an aid in the biological control of the alfalfa caterpillar. Hilgardia 20:359–381
Dulmage HT (1970) Production of the spore-δ-endotoxin complex by variants of Bacillus thuringiensis in two fermentation media. J Invertebr Pathol 16:385–389
Menn JJ (1960) Bioassay of a microbial insecticide containing spores of Bacillus thuringiensis. J Insect Pathol 2:134–138
Krieg A (1965) Über die vivo-titration von insektenpathogenen, speziell von Bacillus thuringiensis. Entomophaga 10:3–20
Burges HD (1967) The standardization of products based on Bacillus thuringiensis. In: van der Laan (ed) Insect pathology and microbial control. North Holland Publication Co, Amsterdam, pp 306–314
Dulmage HT, Rhodes RA (1971) Production of pathogens in artificial media. In: Burges HD, Hussey NW (eds) Microbial control of insects and mites. Acad Press, London, pp 507–540
Becker N, Margalit J (1993) Use of Bacillus thuringiensis israelensis against mosquitoes and blackflies. In: Entwistle P, Cory J, Bailey M, Higgs S (eds) Bacillus thuringiensis. An environmental biopesticide: theory and practice. Wiley, pp 147–170
Goldberg LH, Margalit J (1977) A bacterial spore demonstrating rapid larvicidal activity against Anopheles sergenti, Uranotaenia unguiculata, Culex univattatus, Aedes aegyptü and Culex pipiens. Mosq News 37:355–358
Margalit J, Dean D (1985) The story of Bacillus thuringiensis israelensis. J Am Mosq Control Assoc 1:1–7
Padua LE, Ohba M, Aizawa K (1984) Isolation of Bacillus thuringiensis strain (serotype 8a: 8b) highly and selectively toxic against mosquito larvae. J Invertebr Pathol 44:12–17
Keller B, Langenbruch G (1993) Control of coleopteran pests by Bacillus thuringiensis. In: Entwhistle P, Cory J, Bailey M, Higgs S (eds) Bacillus thuringiensis. An environmental biopesticide theory and practice. Wiley, pp 171–191
Huger AM, Krieg A (1989) Über zwei typen parasporaler kristalle beim käferwirksamen stamm BI. 256-82 von Bacillus thuringiensis subsp. tenebrionis. J Appl Entomol 108:490–497
Krieg A, Huger AM, Langenbruch GA, Schnetter W (1983) Bacillus thuringiensis var. tenebrionis: ein neuer gegenüber larven von Coleopteran wirksamer Pathotyp. Z ang Ent 96:500–508
Höfte H, Whitely H (1989) Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol Rev 53:242–255
Agaisse H, Lereclus D (1995) How does Bacillus thuringiensis produce so much insecticidal crystal protein? J Bacteriol 177:6027–6032
Lereclus D, Agaisse H, Gominet M, Chaufaux J (1995) Overproduction of encapsulated insecticidal crystal proteins in a Bacillus thuringiensis spo0A mutant. Biotechnology (N Y) 13:67–71
Lecadet M, De Barjac H (1981) Bacillus thuringiensis beta-exotoxin. In: Davidson E (ed) Pathogenesis of invertebrate microbial diseases. Allanheld Osmun and Co., New Jersey, pp 293–316
Salama HS, Sharaby A, Ragaei M (1983) Chemical changes in the haemolymph of Spodoptera littoralis as affected by Bacillus thuringiensis. Entomophaga 28:331–337
Salama HS (1984) Bacillus thuringiensis Berliner and its role as a biological control agent in Egypt. Z ang Ent 98:206–220
Boctor I, Salama H (1983) Effect of Bacillus thuringiensis on the lipid content and composition of Spodoptera littoralis larvae. J Invertebr Pathol 41:381–384
Salama HS, Sharaby A (1985) Histopathological changes in Heliothis armigera infected with Bacillus thuringiensis as detected by electron microscopy. Insect Sci Appl 4:503–511
Sutter GR, Raun ES (1967) Histopathology of European corn borer larvae, treated with Bacillus thuringiensis. J Invertebr Pathol 9:90–103
Reese J, Yonke T, Fairchild M (1972) Fine structure of the midgut epithelium in larvae of Agrotis ypsilon. J Kansas Entomol Soc 45:242–251
Cheung P, Grula EA, Burton RL (1978) Haemolymph responses in Heliothis zea to inoculation with Bacillus thuringiensis or Micrococcus lysodeikticus. J Invertebr Pathol 3:148–156
Salama HS, Foda MS, Dulmage HT, El-Sharaby A (1983) Novel fermentation media for production of δ-endotoxins from Bacillus thuringiensis. J Invertebr Pathol 41:8–19
Salama HS, Foda MS, El-Sharaby A, Selim M (1983) A novel approach for whey recycling in production of bacterial insecticides. Entomophaga 28:151–160
Salama HS, Foda MS, Selim MH, El-Sharaby A (1983) Utilization of fodder yeast and agro-industrial byproducts in production of spores and biologically active endotoxins from Bacillus thuringiensis. Zentralblatt Mikrobiol 138:553–563
Salama HS, Morris O (1993) The use of Bacillus thuringiensis in developing countries. In: Entwistle P, Cory J, Bailey M, Higgs S (eds) Bacillus thuringiensis. An environmental biopesticide: theory and practice. Wiley, pp 237–253
Dulmage HT (1971) Production of δ-endotoxin by eighteen isolates of Bacillus thuringiensis serotype 3, in 3 fermentation media. J Invertebr Pathol 18:353–358
Nagamma MV, Ragnathan AN, Majumder SK (1972) A new medium for Bacillus thuringiensis Berliner. J Appl Bact 35:367–370
Fernandez WL, Ocampo TA, Perez DC (1974) Coconut water in three media reduces cell yield of Bacillus thuringiensis var. thuringiensis. Philipp Agric 28:273–279
Foda MS, Salama HS, Fadel M (1993) Local production of Bacillus thuringiensis in Egypt. Advantages and constraints. In: Salama H, Morris O, Rached E (eds) The biopesticide Bacillus thuringiensis and its application in developing countries, pp 149–165
Madkour MA (2000) Egypt: biotechnology from laboratory to the marketplace: challenges and opportunities. In: Persley HJ, Lantin MM (eds) Agriculture biotechnology and the poor: proceeding of an international conference, Washington DC, 21–29 Oct
Salama HS, Foda S, Zaki F, Khalafallah A (1983) Persistance of Bacillus thuringiensis Berliner spores in cotton cultivations. Z ang Ent 95:321–326
Salama HS, Zaki F (1985) Application of Bacillus thuringiensis Berliner and its potency for control of Spodoptera littoralis (Boisd.). Z ang Ent 99:425–431
Ragaei M (1985) Studies on the effect of some environmental and chemical factors on the potency of Bacillus thuringiensis against some cotton pests. M.Sc. thesis, Cairo University, Egypt
Morris O, Moore A (1975) Studies on the protection of insect pathogens from sunlight inactivation II. Preliminary field trials. Report Cc-x-113, Chemical Control Research Institute, 34 pp (8)
Hamed A, Hassanein F (1985) Persistance and virulence of Bacillus thuringiensis under sunny and shady conditions. Bull Entomol Soc Egypt Econ Ser 14:73–77
Morris O (1983) Protection of Bacillus thuringiensis from inactivation by sunlight. Can Entomol 115:1215–1227
Salama HS, Foda S, Selim M (1984) Isolation of Bacillus thuringiensis mutants resistant to physical and chemical factors. Z ang Ent 97:139–145
Salama HS, Foda S, Selim M (1984) Mutation in relation to sporulation and potency of Bacillus thuringiensis vs. cotton pests. Z ang Ent 97:29–36
Matter M (1993) Bacillus thuringiensis and environmental safety. In: Salama HS, Morris O, Rached E (eds) The biopesticide Bacillus thuringiensis and its applications in developing countries, pp 257–265
Salama HS, Zaki FN, Sharaby AF (1982) Effect of Bacillus thuringiensis Berl. on parasites and predators of the cotton leafworm Spodoptera littoralis (Boisd.). Z ang Ent 94:498–504
Salama HS, Zaki FN (1983) Interaction between Bacillus thuringiensis Berliner and the parasites and predators of Spodoptera littoralis in Egypt. Z ang Ent 95:425–429
Salama HS, Zaki FN (1984) Impact of Bacillus thuringiensis Berl. on the predator complex of Spodoptera littoralis (Boisd.) in cotton fields. Z ang Ent 97:485–490
Salama HS, Zaki FN (1985) Biological effects of Bacillus thuringiensis on the egg parasitoid Trichogramma evanescens. Insect Sci Appl 6:145–148
Afify AM (1964) Bioassay of three bacterial insecticides on the base of Bacillus thuringiensis Berliner, using its original host Anagasta kuehniella as a test insect. Bull Soc Entomol Egypt 48:103–109
Afify AM (1965) Studies on the susceptibility of certain stored products insects to bacterial insecticides: 1-tests with “Bakthane L-69”. Bull Soc Entomol Egypt 49:59–64
Afify AM (1968) Bioassay of “Biospore 2902” using two species of lepidopterous larvae of different susceptibility levels. J Invertebr Pathol 10:283–286
Afify AM, Merdan AI (1969) On tracing the response of some Egyptian cotton worms in different larval ages to Bacillus thuringiensis Berliner. Z ang Ent 63:263–267
Afify A, Hafez M, Merdan A (1969) Preliminary investigations on the virulence of 13 Bacillus preparations against 3 Egyptian noctuids. Anz Schadlingsk Pflanz Unwelt 42:54–57
Afify AM, El-Sawaf S, Habib E, Hammad SM (1970) Pathogenicity tests of Biotrol BTB process 183, on Anagasta kuehniella Zeller. Z ang Ent 65:29–37
Soliman A, Afify A, Abdel-Rahman H, Atwa W (1970) Effectiveness of different components of Bacillus thuringiensis against three larval stages of Pieris rapae. Anz Schadlingsk Pflanz Umwelt 43:161–165
Salama HS, Foda MS, El-Sharaby A (1981) Potency of spore endotoxin complexes of Bacillus thuringiensis against some cotton pests. Z ang Ent 91:388–398
Salama HS, Foda MS (1982) A strain of Bacillus thuringiensis var. entomocidus with high potential activity on Spodoptera littoralis. J Invertebr Pathol 39:110–111
Salama HS, Foda MS (1984) Studies on the susceptibility of some cotton pests to various strains of Bacillus thuringiensis. J Plant Dis Prot 91:65–70
Abul-Nasr S, Abdallah MD (1969) Lethal and sublethal action of Bacillus thuringiensis Berliner on the cotton leafworm Spodoptera littoralis (Biosd.). Bull Entomol Soc Egypt Econ Ser 4:151–160
Abdallah MD, Abul-Nasr S (1970) Feeding behavior of the cotton leafworm Spodoptera littoralis (Boisd.) sublethally infected with Bacillus thuringiensis Berliner. Bull Entomol Soc Egypt Econ Ser 4:161–170
Abdallah MD, Abul-Nasr S (1970) Effect of Bacillus thuringiensis Berliner on reproductive potential of the cotton leafworm (Lep., Noctuidae). Bull Entomol Soc Egypt Econ Ser 4:171–176
Afify AM, Matter MM (1969) Retarded effect of Bacillus thuringiensis Zell. Entomophaga 14:447–456
Afify A, Hafez M, Matter M (1971) The retarding effect of Bacillus thuringiensis on larval development of flour moth Anagasta kuehniella with a new method of determining the duration of instars. Acta Entomol Bohemoslov 68:6–14
Matter M, Zohdy N (1981) Biotic efficiency of Bacillus thuringiensis and a nuclear polyhedrosis virus on larvae of the American bollworm Heliothis armigera. Z ang Ent 92:336–343
Salama HS, Sharaby A (1988) Effects of exposure to sublethal levels of Bacillus thuringiensis on the development of the greasy cutworm Agrotis ypsilon (Hbn). Z ang Ent 106:396–401
Salama HS, Foda S, Zaki F, Ragaei M (1986) On the distribution of Bacillus thuringiensis and closely related Bacillus cereus in Egyptian soils and their activity against Lepidopterous cotton pests. Z ang Zool 73:257–265
Abdel-Rahman H (1966) Study of the pathogenicity of crystalline inclusion of Bacillus thuringiensis. Ain Shams Sci Bull 10:89–95
Abul-Nasr S, Ammar E, Merdan A, Farrag S (1979) Infectivity tests on Bacillus thuringiensis and B. cereus isolated from resting larvae of Pectinophora gossypiella. Z ang Ent 88:60–69
Abdel Ghany N (2006) Studies on the potential activity and molecular characterization of Bacillus thuringiensis isolated from Lepidopterous cotton insects and soil in Egypt. M.Sc. thesis, Ain Shams University, Egypt
Delucca A, Simonsen JG, Larson A (1981) Bacillus thuringiensis distribution in soils of the United States. Can J Microbiol 27:865–870
Anwar M, Sohel A, Sirajul H (1997) Abundance and distribution of Bacillus thuringiensis in the agricultural soil of Bangladesh. J Invertebr Pathol 70:221–225
Morris O, Converse V, Kanagaratnam P, Cote J (1998) Isolation, characterization and culture of Bacillus thuringiensis from soil and dust from grain storage bins and their toxicity for Mamestra configurata. Can Entomol 130:515–537
Ali A, Watson T (1982) Effect of Bacillus thuringiensis var. kurstaki on tobacco budworm adult and egg stages. J Econ Ent 75:596–598
Potter M, Jensen M, Watson T (1983) Influence of sweet bait Bacillus thuringiensis var. kurstaki combinations on adult tobacco budworm. J Econ Entomol 75:1157–1160
Salama HS (1985) Control of Spodoptera littoralis through moth and eggs treatment with Bacillus thuringiensis. Insect Sci Appl 6:49–53
Salama HS, Zaki F (1986) Effects of Bacillus thuringiensis Berliner on prepupal and pupal stages of Spodoptera littoralis. Insect Sci Appl 7:747–749
Abdallah M, Zaazou H, El-Tantawi M (1974) Wirkung eines Bacillus thuringiensis preparats und eines Juvenile hormone – Analogous über dem erdboden auf Spodoptera littoralis. Anz Schadlingsk Pflanz Umwelt 47:170–172
Salama HS, Foda MS, Zaki FN, Moawad S (1984) Potency of combinations of Bacillus thuringiensis and chemical insecticides on Spodoptera littoralis (Lepidoptera: Noctuidae). J Econ Entomol 77:885–890
Abdallah M (1969) The joint action of microbial and chemical insecticides on the cotton leafworm Spodoptera littoralis. Bull Entomol Soc Egypt Econ Ser 3:201–217
Altahtawy M, Abaless I (1972) Thuricide 90 Ts flowable, a recent approach to the biological control of Spodoptera littoralis. Z ang Ent 72:299–308
Altahtawy M, Abaless I (1973) An integrated control trial of Spodoptera littoralis (Boisd.) using Bacillus thuringiensis associated with insecticides. Z ang Ent 74:255–263
Salama HS, Moawed S, Zaki F (1987) Effects of nuclear polyhedrosis virus—Bacillus thuringiensis combinations on Spodoptera littoralis (Roisd.). Z ang Ent 104:22–27
Salama HS, Foda S, El-Sharaby A (1983) Biological activity of mixtures of Bacillus thuringiensis against some cotton pests. Z ang Ent 95:69–74
Salama HS, Foda S, Sharaby A (1985) Role of feeding stimulants in increasing the potency of Bacillus thuringiensis vs. Spodoptera littoralis. Entomol Gener 10:111–119
El-Nockrashy S, Salama HS, Taha F (1984) Developemt of bait formulations for control of Spodoptera littoralis. Z ang Ent 103:313–319
Charles C, Wallis R (1964) Enhancement of the action of Bacillus thuringiensis var. thuringiensis on Porthetria dispar (Linn.) in laboratory tests. J Insect Pathol 6:423–429
Smirnoff WA (1974) The symptoms of infection by Bacillus thuringiensis and chitinase formulation in larvae of Choristoneura fumiferana. J Invertebr Pathol 23:397–399
Burges HD (1977) Control of the waxmoth Galleria mellonella on beecomb by H-serotype v Bacillus thuringiensis and the effect of chemical additives. Apidologie 8:155–168
Couch TL, Ross D (1980) Production and utilization of Bacillus thuringiensis. Biotechnol Bioeng 22:1297–1304
Narayanan K, Govindarajan R, Jayaraj S (1976) Role of alkali components and gut microflorae of Papilio demoleus L. and Spodoptera litura F. in the mode of action of Bacillus thuringiensis Berliner. Madras Agric J 64:344–346
Nickerson KW (1980) Structure and function of Bacillus thuringiensis protein crystal. Biotechnol Bioeng 22:1305–1333
Dixon M, Webb EC (1964) Enzymes. Academic Press Inc., New York, pp 67–70
Endo Y, Nishitsuji-Uwo (1980) Mode of action of Bacillus thuringiensis δ-endotoxins: histopathological changes in the silkworm midgut. J Invertebr Pathol 36:90–103
Wigglesworth VB (1972) The principles of insect physiology. English Language Book Society, Chapman Hall, England
Patti H, Carver GR (1974) Bacillus thuringiensis investigation for control of Heliothus spp. on cotton. J Econ Entomol 67:415–418
Salama HS, Foda S, El-Sharaby A, Matter M, Khalafallah M (1981) Development of some lepidopterous cotton pests as affected by exposure to sublethal levels of endotoxins of Bacillus thuringiensis for different periods. J Invertebr Pathol 38:220–229
Salama HS, Salem S, Zaki F, Matter M (1990) Control of Agrotis ypsilon on some vegetable crops in Egypt using the microbial agent Bacillus thuringiensis. Anz Schadlingsk Pflanz Umwelt 63:147–151
Salama HS, Salem S, Matter M (1991) Field evaluation of the potency of Bacillus thuringiensis on lepidopterous insects infesting some field crops in Egypt. Anz Schadlingskd Pflanz Umwelt 64:150–154
Pilcher CD, Rice M, Obrycki J, Lewis L (1997) Field and laboratory evaluation of transgenic Bacillus thuringiensis corn on secondary Lepidopteran pests (Lepidoptera: Noctuidae). J Econ Entomol 90:669–678
Assem SK (2014) Opportunities and challenges of commercializing biotech products in Egypt: Bt. maize, a case study. In: Wambugu F, Kammanga D (eds) Biotechnology in Africa: emergence, initiatives and future, pp 37–47
El-Banna H (2011) Terza Giornata Mondiale del Mais, Bioenergy, Italy. Maize production in Egypt. Al-Ahram Agriculture, Cremona, 18–20 Mar 2011
Saker M, Salama HS, Salama M, El-Banna A, Abdel-Ghany N (2011) Production of transgenic tomato plants expressing Cry 2 Ab gene for the control of some lepidopterous insects endemic in Egypt. J Genetic Eng Biotechnol 9:149–155
Salama HS, Foda S, Sharaby A (1984) Novel biochemical avenues for enhancing Bacillus thuringiensis endotoxin potency against Spodoptera litoralis. Entomophaga 29:171–178
Salama HS, Foda S, Sharaby A (1986) Possible extension of the activity spectrum of Bacillus thuringiensis strains through chemical additives. J Appl Ent 101:304–313
Salama HS (1993) Enhancement of Bacillus thuringiensis for field application. In: Salama HS, Morris O, Rached E (eds) The biopesticide Bacillus thuringiensis and its application in developing countries. Al-Ahram Press, Cairo, pp 105–116
Osman GEH, Already R, Assaeedi ASA, Organji SR El-Ghareeb D, Abulreesh HH, Althubiani AS (2015) Bioinsecticide Bacillus thuringiensis a comprehensive review. Egypt J Biol Pest Control 25:271–288
Acknowledgements
The author is greatly indebted to Dr. I. Shehata and Miss Dalal Ali, in NRC for their sincere efforts and help during the preparation of this book chapter.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Nature Switzerland AG
About this chapter
Cite this chapter
Salama, H.S. (2020). Production and Application of Bacillus thuringiensis for Pest Control in Egypt. In: El-Wakeil, N., Saleh, M., Abu-hashim, M. (eds) Cottage Industry of Biocontrol Agents and Their Applications. Springer, Cham. https://doi.org/10.1007/978-3-030-33161-0_6
Download citation
DOI: https://doi.org/10.1007/978-3-030-33161-0_6
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-33160-3
Online ISBN: 978-3-030-33161-0
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)