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Tissue Optics

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The Optical Clearing Method

Part of the book series: SpringerBriefs in Physics ((SpringerBriefs in Physics))

Abstract

Light interaction with biological materials depends on the material’s optical properties. From those properties, the absorption and scattering coefficients are the most important, since they quantify how much of a light beam is attenuated when traveling inside a tissue. The scattering coefficient is known to be significantly higher than the absorption coefficient in biological materials, meaning that most of the light is scattered, turning optical methods in clinical practice limited. Such difference between the scattering and absorption coefficients is mainly due to a refractive index mismatch between tissue components and fluids. We explain this concept in the present chapter before introducing the technique that efficiently minimizes this effect in the following chapters.

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Authors and Affiliations

  1. Physics Department and Center for Innovation in Engineering and Industrial Technology, Polytechnic Institute of Porto – School of Engineering, Porto, Portugal

    Luís Manuel Couto Oliveira

  2. Department of Optics and Biophotonics, Saratov State University, Saratov, Russia

    Valery Victorovich Tuchin

  3. Institute of Precision Mechanics and Control of the RAS, Saratov, Russia

    Valery Victorovich Tuchin

  4. Bach Institute of Biochemistry, Research Center of Biotechnology of the RAS, Moscow, Russia

    Valery Victorovich Tuchin

  5. Tomsk State University, Tomsk & ITMO University, St. Petersburg, Russia

    Valery Victorovich Tuchin

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  1. Luís Manuel Couto Oliveira
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Oliveira, L.M.C., Tuchin, V.V. (2019). Tissue Optics. In: The Optical Clearing Method. SpringerBriefs in Physics. Springer, Cham. https://doi.org/10.1007/978-3-030-33055-2_1

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