Anaerobic Digestion of Rice Straw for Biogas Production
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Anaerobic digestion (AD) is a process of degradation of organic matter by microorganisms in an oxygen-free environment, which produces biogas, a vital renewable energy source. Using solely an organic source, such as monosubstrates, it is difficult to optimize the AD process due to nutrient imbalance, lack of appropriate microbial communities, and the effect of operational parameters. This chapter reviews the current studies on biogas production from the anaerobic codigestion process of mixing agricultural byproducts, focusing on rice straw and livestock manure as substrates. Because rice straw is high in cellulose, it needs to be pretreated before feeding into the anaerobic digester. Different rice straw pretreatments are summarized including physical, chemical, and biological methods. Current biogas systems are discussed. The utilization of bioslurry from the anaerobic fermentation process to agricultural cultivation and aquaculture activities is also discussed.
KeywordsAnaerobic digestion Biogas Co-digestion Pre-treatment Rice straw
5.1 Introduction of Anaerobic Digestion Technology
5.1.1 Products of Anaerobic Digestion
The composition of biogas obtained from organic substances
The composition of biogas (%)
Rasi et al. (2007)
Allen et al. (1997)
Jönsson et al. (2003)
Rasi et al. (2007)
Rasi et al. (2007)
Tran et al. (2014)
Jönsson et al. (2003)
Spiegel and Preston (2003)
Polprasert and Koottatep (2007)
Tran et al. (2014)
Biogas technology is applicable to small-scale and large-scale uses including electricity generation.
Biogas can also be upgraded into biomethane or renewable natural gas (RNG), which is like natural gas that comes from fossil fuels. Its methane content is 90% or greater. RNG can be substituted for natural gas and can be used as fuel for vehicles that run on natural gas and to supply gas to natural gas grid.
Aside from biogas, the other AD byproduct is digestate. It can be solid or liquid and contains considerable amounts of nitrogen (in ammonium form), macronutrients, and micronutrients that can supplement plant growth (Makádi et al. 2012). Depending on the feedstock of AD, digestate can be utilized as an organic fertilizer or as a compost ingredient. Digestate that uses plant and animal-based feedstocks can be used directly as a fertilizer. One example is the VACB model (Vườn/Garden-Ao/Pond-Chuồng/Pigsty-Biogas) of Vietnam (Thanh 2010). Digestate from the AD process that uses an organic fraction of municipal waste as feedstock will need further processing or will need to be disposed of in a landfill because of possible high levels of heavy metals, pathogens, and other toxic substances.
5.1.2 Anaerobic Digestion Process
220.127.116.11 Stage 1: Hydrolysis
Insoluble organic compounds, such as cellulose, protein, fat, and some insoluble forms of organic compounds, are decomposed by enzymes (produced by bacteria) and anaerobic bacteria. Small soluble organic molecules, which are produced in this stage, are the raw material for the bacteria in the next stage. Hydrolysis of carbohydrates can occur within a few hours, while protein and fat hydrolysis may take several days. However, lignocellulose and lignin substances decompose slowly and incompletely (Deublein and Steinhauser 2011). Facultative anaerobes consume dissolved oxygen in water, which results in reduction of redox potential that is favorable to the AD process. In this stage, carbohydrates are broken down into simple sugars; fats degrade into fatty acids; and proteins degrade into amino acids (Gerardi 2003; Eastman and Ferguson 1981).
18.104.22.168 Stage 2: Acid-Producing (Acidogenesis)
Simple organic compounds which were created during hydrolysis stage will be transformed into volatile fatty acids (VFAs), long chain fatty acids, propionate, and butyrate by anaerobes (Jördening and Winter 2006). The concentration of H+ formed in this stage may affect the products of fermentation. High concentration of H+ reduces the production of acetate. In general, during this stage, simple sugars, fatty acids, and amino acids are fermented to form organic acids and alcohol (Gerardi 2003).
22.214.171.124 Stage 3: Acetic Acid-Producing (Acetogenesis)
The products from the previous stage are the substrate for bacteria in the acetic acid-producing stage. The products from these intermediate substrates are H2, CO2, and acetate. Acetogenic bacteria grow together with methanogen bacteria in this stage.
126.96.36.199 Stage 4: Methane-Producing (Methanogenesis)
Some methanogenesis reactions
4HCO2−+H+ + H2O
Many hydrogenotrophic methanogens
2CH3COO−+H+ + CH4+H2O
Methanosarcina and Methanothrix
Methanosarcina and other methylotrophic methanogens
5.1.3 Factors Affecting the Anaerobic Digestion Process
Factors that affect the AD process
Optimum value for methane production
1–4 kg VS m3 day−1
1–4 kg VS m3 day−1
Psychrophilic anaerobic digestion (<20 °C)
Mesophilic anaerobic digestion (20–45 °C)
Thermophilic anaerobic digestion (46–60 °C)
The activity of methane-forming bacteria is strongly influenced by temperature. In general, when the temperature increases, biogas production increases. For that reason, digesters are heated in colder regions, such as Europe. But at a temperature range of 40–50 °C, biogas production will decrease because this temperature range is not suitable for both mesophilic and thermophilic bacteria. If the temperature is above 60 °C, biogas production decreases and it stops completely at 65 °C or higher. Biogas production is at its maximum rate when the temperature is kept at 35 °C (Chandra et al. 2012), while optimum temperatures for methane production range from 35 to 40 °C (Lianhua et al. 2010).
Temperature is a very important variable for efficient anaerobic digestion of rice straw. Maximized methane production and economic input depend on it.
188.8.131.52 pH and Alkalinity
McCarty (1964) determined that the biological process of AD works optimally in a pH environment of 6.6–7.6. However, Yadvika et al. (2004) and Gerardi (2003) reported that the optimum pH range for AD is at 6.8–7.2. At a pH lower than 5.5, acidogenic bacteria are still active but methanogenic bacteria are inhibited. Inhibition of methanogenic bacteria lowers the methane content of the biogas; therefore, low pH conditions are avoided. During the AD process, pH will usually drop lower than 6.6 if there is excessive accumulation of fatty acids in the acidogenesis stage. Decrease in pH is caused by either overloading of substrates or because the toxins in the feedstock inhibit the activity of methanogens. In this case, substrate feed must be stopped so that acid production will stop or decrease and acetogens and methanogens will be able to degrade excess acid that was produced. Another solution is to use lime for neutralizing the acid and increase the pH to an optimum range. An increase in pH (greater than 8.0) also has an inhibitory effect on AD. At pH 9.0, the methanogenesis process completely stops (Clark and Speece 1971).
Alkalinity is a measure of the capacity of a solution to neutralize acid. Bicarbonate (HCO3−), carbonate (CO32−), and hydroxide (OH−) are the ions that are used to increase alkaline conditions in the digester. Alkalinity is also considered as the buffering capacity of a solution and is essential for controlling and maintaining a stable pH for the anaerobic digestion process. High alkalinity conditions (1500–3000 mg CaCO3 L−1) enhance the pH stability of the anaerobic digestion process (Gerardi 2003).
184.108.40.206 Redox Potential
The redox potential is a measure of oxidation capacity or reducing capacity. Biogas is produced effectively in an anaerobic environment where the redox potential must be less than −150 mV. Redox always reaches a negative value (less than −100 mV) under anaerobic conditions (Wiese and König 2009).
In general, the use of substrates including oxygen, nitrate and sulfate promotes oxidation that can significantly change the redox potential and cause changes in pH. Redox potential can be used to predict impending changes in pH of the digester (Wiese and König 2009).
Methane begins to form and CO2 and H2 are converted into CH4 when the redox value is less than −250 mV; H2O and H2S are produced when the redox value is less than −150 mV (Laanbroek 1990).
High salinity and ammonium concentrations have detrimental effects on biological processes such as anaerobic digestion (Fang et al. 2011; Chen et al. 2008; Reinhart and Townsend 1998; Kargi and Dincer 1996). High salt concentrations dehydrate bacterial cells due to osmotic pressure (Alhraishawi and Alani 2018). Salt toxicity is determined mostly by the type of cation the salt has.
Feedstock inflow to anaerobic digesters usually contains light metal ions, namely, sodium, potassium, calcium, and magnesium. These cations may also be liberated during the AD process (Chen et al. 2007). A study by Albraishawi and Alani (2018) on codigestion of food waste demonstrated that increasing salt concentrations (0, 16, 30, and 60 g NaCl L−1) has a negative effect on the volume of biogas produced (45, 21, 5, and 2 ml d−1). In terms of methane yield, a study by Lee et al. (2009) on anaerobic digestion of leachate from a food waste recycling facility showed that low-salt concentrations (0.5 and 2 g NaCl L−1) increase methane yield, but higher salt concentrations (5 and 10 g NaCl L−1) resulted in a decrease in methane yield (36 and 41% reduction).
Anwar et al. (2016) showed that methane yield inhibition in anaerobic digestion of food waste is negligible at salt concentrations of 8 g NaCl L−1, but salt concentrations greater than 8 g Nacl L−1 resulted in a sharp decline in methane yield. The cubic regression model y = 0.508 + 2.401x – 0.369x2 + 0.033x3 was derived from this experiment to describe sodium salt inhibition, where y is the methane yield and x is the sodium salt concentration. This model predicted experimental results with a small discrepancy of 10%. A study by Ogata et al. (2016) on the effect of salt on biogas production of leachate in a waste landfill showed that methane production decreased while carbon dioxide production was unchanged at a salt concentration of 35 ms cm−1 (approximately 19 mg L−1). A salt content of 80 ms cm−1 (approximately 44 mg L−1) decreased production of both methane and carbon dioxide. Based on these studies on salt inhibition of the anaerobic digestion process, it can be inferred that low salt concentrations in the AD reactant mixture (up to 2 g L−1 NaCl) increase methane yield, but higher salt concentrations (greater than 5 g L−1 NaCl) decrease methane yield.
220.127.116.11 Carbon to Nitrogen (C/N) Ratio
C/N ratio of some AD substrates
Ngan et al. (2011)
The difference in C/N ratios show that plant materials have a high C/N ratio and animal manures have a low one. To achieve the optimum C/N ratio of 20/1–30/1, plant material and animal manures are codigested.
18.104.22.168 Loading Rate and Hydraulic Retention Time (HRT)
The AD process is affected by the loading rate, represented by chemical oxygen demand per cubic meter per day (COD m−3 day−1) or volatile solids per cubic meter per day (VS m−3 day−1), and hydraulic retention time (HRT) of the fermentation mixture. A high organic loading rate will cause accumulation of fatty acids at stage 3, which negatively affects the production of methane. A low organic loading rate on the other hand will produce low volumes of biogas that will make AD operation uneconomical. The recommended organic loading rate for an anaerobic tank without medium is 1–4 kg V m−3 day−1 (Eder et al. 2006). HRT is the time that the substrates stay inside the anaerobic digester reactor. HRT strongly depends on the substrates and it usually varies from 30 to 60 days. In general, 30 days are typical HRT for nonstirring digesters, the digesters with high decomposition rates can be reduced to an HRT of 10–20 days.
Toxic dose of substances on AD
Toxic dose for AD bacteria
Volatile fatty acids
>10,000 mg L−1
Wang et al. (2009)
>1700 mg L−1
Angelidaki and Ahring (1992)
>1000 mg L−1
Angelidaki and Ahring (1992)
>16,000 mg L−1
Koster and Lettinga (1988)
>145 mg L−1
Parkin et al. (1990)
>7000 mg L−1
Ahn et al. (2006)
>1000 mg L−1
>8000 mg L−1
Kugelman and McCarty (1965)
>3500 mg L−1
>5 mg L−1
22.214.171.124 Dry Matter and Water Content
The percentage of dry matter content in a digester suitable for biogas generation and solids reduction is about 9–10%. The concentration of dried matter up to 20% helps to save 50% of the volume of digesters but may lead to souring (reduction of pH) and consequently, reduction of biogas output. Monnet (2003) recommended that the organic dry matter content of batch digester should be adjusted to a range of from 5% to 10%.
Stirring maximizes contact of bacteria with organic waste to accelerate the digestion process. It also minimizes the solids deposition on the bottom of the digesters and avoids foaming and scum on the fermentation solution surface.
During the digestion process, it is necessary to mix the substrates in the digester, especially for the substrate from plant biomass, to avoid scum formation. Mixing increases the contact between bacteria and substrate and improves decomposition. Mixing can also prevent foaming and make the temperature uniform inside the digester. However, a fast mixing speed will break down the microbial population, thus it is best to stir at lower mixing speeds (Monnet 2003).
126.96.36.199 Feedstock Pretreatment
Fibrous substrates—especially straw, grass, weeds, and stalks—are difficult to decompose and must be treated before digestion. Pretreatment aims to reduce the crystallization of cellulose, increase the surface area of the substrate, and make cellulose more accessible to enzymes that convert carbohydrates into fermentable sugars. Pretreatment includes physical, chemical, biological, and their combinations (Alvira et al. 2010).
The anaerobic fermentation process can be enhanced if the digestate is recycled and mixed with substrate feed. The solids content of feeding materials should be adjusted to 5–10%.
188.8.131.52 Feedstock Size
Feeding material size is one of the factors affecting biogas production. Materials should not be too large because it will lead to digester blockage and will also make it difficult for bacteria to decompose. Small-sized materials will have larger surface areas and increased microbial activity, resulting in faster decomposition. According to Sharma et al. (1988),when substrates having five different particle sizes (0.088, 0.4, 1.0, 6.0, and 30.0 mm) were digested and their biogas output were compared, it was shown that the optimal size for producing biogas was 0.088 and 0.4 mm. Some other studies also suggested that a physical pretreatment method, such as grinding and chopping, can significantly reduce digester size design as compared with digesters using untreated substrates,without reducing the biogas production (Moorhead and Nordstedt 1993; Gollakota and Meher 1988).
5.2 AD Systems
5.2.1 Small-Scale Biogas Digesters
Small-scale biogas plants have mostly been popularized in developing countries. In many of those countries, massive government-led (China, India) or government-NGO biogas programs were initiated with the aim of popularizing this technology.
Constructed on-site plants: these plants are often made of brick, mortar and concrete.
Prefabricated plants (PBD): these plants are produced off-site and installed at the farms. They are made of fiber-reinforced plastic (FRP), tubular (known as bag plants or soft plastic plants), and hard plastics, such as hard polyvinyl chloride (PVC), acrylonitrile butadiene styrene (ABS), high-density polyethylene (HDPE), linear low-density polyethylene (LLDPE), and modified plastics.
184.108.40.206 Biogas Plants Constructed On-Site
Comparison of digester plants constructed on-site
Top manhole (Chinese plant types)
Easy to enter the digester from the top
Top manhole is situated on top of gasholder and is prone to leakages
Can withstand high pressures, therefore, can store more gas
Gas proofing of the dome requires special paints and skilled masons
Manhole at the outlet (Deenbandhu, Janata)
Has the highest load bearing capacity as the dome is a closed solid structure
Gas proofing of the dome requires special paints and skilled masons
Cheaper than Chinese digester models due to optimized shape
Drum is gastight
Higher investment and additional transport cost of drum
Lower masonry skill requirement
Distance between inlet and outlet is relatively short which can result in preferential flow of manure shortening the HRT
High depth to width ratio which makes construction difficult in certain soils
220.127.116.11.1 Fixed-Dome Digesters
The archetype of all the on-site constructed hydraulic biodigesters is the water pressure digester of Luo Guorui developed in the 1920s in Taiwan (Nianguo 1984). This design eventually led to the Chinese fixed-dome digester. The gas pressure varies depending on the amount of slurry accumulated in the displacement tank (or compensation / hydraulic chamber). The pressure is at its highest when the gasholder is full of gas when an equivalent volume of slurry is push upward into the displacement tank; and the pressure is 0 when all gas is used. Gas pressure typically varies between 2 and 15 kPa (Gunnerson et al. 1986).
18.104.22.168.2 Indian Digesters
The Deenbandhu digester is the archetype of all fixed-dome digesters with a manhole at the outlet tank. The digester can resist higher structural forces compared to the Chinese digester types as the dome is a closed structure. Another advantage is that the gasholder is closed that reduces the risk of leaks compared to a Chinese digester, which has a manhole at the top of the gasholder (Balasubramaniyam et al. 2008).
22.214.171.124.3 Floating Drum Digesters
The floating drum digester was introduced by the Khadi and Village Industry Commission and was later branded as the KVIC digester (Singh and Sooch 2004). The floating drum digester is fed semicontinuously and has a relatively high depth-to-width ratio. A barrier is placed in the middle of the digester to promote mixing and prevent short-circuiting (direct substrate flow from the entrance to the exit) (Gunnerson et al. 1986). Biogas accumulates at the headspace of a movable inverted steel drum that serves as the upper part of the digester.
126.96.36.199 Prefabricated On-Site Biogas Plants
188.8.131.52.1 Fiber-Reinforced Plastic (FRP) Digesters
The technical designs of fixed-dome FRP biodigesters can be classified in different ways according to the major features of the digester, such as the full FRP digester and the partial FRP digester (usually upper-dome part only) based on the integrity of FRP structural materials; top-mounted hydraulic chamber and lateral-placed hydraulic chamber based on the placement of hydraulic (displacement) chamber; spherical, ellipsoidal, and cylindrical based on the shape of digester body; modular type and integral type based on assembly work; and differences in digester volume. Sizes of these plants are typically in the range of 4–9 m3.
184.108.40.206.2 Hard-Plastic Digester Types
The most common processes used to produce hard-plastic biodigesters are rotation and injection molding. Injection molding machines are more expensive, but the process allows for more intricate shapes, can utilize more types of materials, and is much faster, resulting in lower production costs. Materials used include hard PVC, ABS, HDPE, LLDPE, and modified plastics.
220.127.116.11.3 Soft-Plastic Digester Types
The archetype of soft-plastic digester types is the Taiwanese bag digester, which was developed in the 1960s. It is a popular model especially in Central and South America (Gunnerson et al. 1986). The digester is made of a cylindrical plastic tank (tube) nested horizontally on a hardened layer of masonry, concrete, sand, or mud (Gunnerson et al. 1986). A great advantage of the bag digester is the simple design and low material costs. However, these low-cost materials often break down easily and often render the effective lifespan of the digester to less than 2 years. In the last decade, SP digester designs emerged that use higher-quality materials, such as geo-membranes and HDPE instead of polyethylene. Such digesters are durable and should have a lifespan of from 6 to 10 years. Lower-quality soft-plastic digesters made from simple PE sheets are also available. These digesters are cheap, but are easily damaged and require frequent repairing and, as such, are not actively promoted.
5.2.2 Medium- and Large-Scale Plants
Two-stage AD is widely used for medium- and large-scale biogas plants with the two digester tanks in series. The advantage of having two stages for AD is the separation of the methanogenesis stage from the acidogenesis stage because acids produced in the acidogenesis stage can inhibit the methanogens. The first tank is optimized for hydrolysis and acidogenesis and the second tank is optimized for acetogenesis and methanogenesis. An example of the two-stage AD power plant is presented in Sect. 18.104.22.168.
Medium- and large-scale biogas plants are used on commercial farms and often produce more biogas than the farming households can use. Consequently, biogas is turned into electricity or supplied to gas grids or fuel stations after being upgraded to a desired purity. Most of the large-scale biogas plants are constructed in Germany, USA, UK, and China. Many of the these plants are generating biogas from landfill, wastewater, fuel crops, and to a lesser extent from livestock manure.
5.3 Current Technology Developments and Practices for Rice Straw AD
5.3.1 Rice Straw Pretreatment for AD
Pretreatment methods to improve the anaerobic digestion process were presented in many studies, such as Ngan (2012), Pilli et al. (2011), Hendriks and Zeeman (2009), Neyens et al. (2003), Weemaes and Verstraete (1998), Stuckey and McCarty (1984), and Haug et al. (1978). Pretreatment has been reported as an important step in the methane production process (Alvira et al. 2010; Carvalheiro et al. 2008; Taherzadeh and Karimi 2008). Pretreatment will change the structure of cellulose so that the enzymes can easily convert high molecular weight molecules, such as carbohydrates into simple sugars (Mosier et al. 2005). Especially in the case of rice straw, a biomass with a high-lignin pretreatment step is necessary to amplify the degradability of rice straw and speed up the anaerobic digestion process.
Rice straw pretreatment methods are classified as physical (particle size reduction), chemical (acid and alkali additions), and biological (fungi).
22.214.171.124 Physical Pretreatment: Effect of Particle Size of Rice Straw
Pretreatment of the feedstock can increase its solubility, consequently increasing biogas production and enhancing reduction of volatiles and solids content. Pretreatment is especially helpful in the digestion of biomass substrates as these substances tend to have high cellulose or lignin content. Additives can increase the production rate of the reactor or increase the startup speed, but their additional cost must always be balanced against improvements in efficiency (Ward et al. 2008; Ngan 2012).
Rice straw particle size reduction breaks the cell walls and makes the organic substrate more readily available for microbes to decompose (Zhang and Zhang 1999). Size reduction of rice straw increases surface area and breaks down its polymer structure, thereby increasing hydrolysis yield and hydrolysis rate during digestion (Hendriks and Zeeman 2009). Gharpuray et al. (1983) verified that pretreatment of wheat straw by ball-milling was found to be effective in increasing specific surface area (2.3 m2 g−1 for pretreated substrate compared to 0.64 m2 g−1 for raw straw). Fiber degradation and methane yield are enhanced when particle size is reduced from 100 mm to 2 (Mshandete et al. 2006). Consequently, methane yield increased (5–25%) and digestion time was reduced (23–59%) (Hendriks and Zeeman 2009). A study by Zhang and Zhang (1999) revealed that rice straw cut in 25-mm lengths has higher methane (198 L kg−1 VS) versus uncut rice straw. However, addition of a milling step in the AD process is expensive due to its high energy requirements (Hendriks and Zeeman 2009).
Møller et al. (2004) observed, for rice straw AD, the increase in methane yield from 30-mm lengths (145 L kg−1 VS added) compared to 1-mm lengths (161 L kg−1 VS added) was significant after 60 days of digestion. Increased bio-degradability of rice straw has been demonstrated when methane production yield was found 7.9 and 13% higher, respectively, for sizes of 0.30 and 0.75 mm compared to 1.5 mm (Chandra et al. 2015). Sharma et al. (1988) reported that methane production tends to increase by from 43.5 to 52.0% when the particle size was gradually reduced from 30 mm to 0.088 mm. However, rice straw sizes under 0.5 mm could be detrimental to the methane production process due to excessive accumulation of volatile fatty acids (VFAs) that will reduce pH in biogas reactor, causing inhibition of anaerobic organisms.
Chopping and grinding rice straw into small particles less than 1 mm in size and mixing with kitchen waste and pig manure could produce methane from 205 to 384 L kg−1 VS added (Ye et al. 2013) whereas 2–3 cm of chopped rice straw with added micronutrients (nickel and cobalt at 10 and 15 mg kg−1, respectively) increased biogas production by 37 and 46% in mesophilic and thermophilic digesters, respectively.
126.96.36.199 Chemical Pretreatment
Chemical pretreatment methods mainly include acid and/or alkaline pretreatments.
188.8.131.52.1 Acid Pretreatment
Hydrolysis by weak acid: acid pretreatment at low concentration is one of the most effective methods for treating lignocellulosic biomass. This method includes two types of hydrolysis: (1) continuous hydrolysis at high temperature (above 160 °C) and a low loading rate (about 5–10% TS), and (2) batch hydrolysis at low temperatures (below 160 °C) with a high loading rate (about 10–40% TS). Acid sulfuric—sprayed into the lignocellulose, mixed, and then kept at from 160 to 200 °C for a few minutes—will remove hemicellulose. As a result, the efficiency of the hydrolysis process will be improved. Acid pretreatment showed the significant improvement of enzyme activity and removed hemicellulose effectively (Chen et al. 2007).
Strong acid pretreatment: High concentrations of H2SO4 and HCl are widely used for pretreatment of lignocellulose because these acids are powerful and they help hydrolyze cellulose (Sun and Cheng 2002) without the involvement of enzymes in the hydrolysis process. The disadvantage of this method is the corrosive properties of these acids and they should be recycled to reduce pretreatment costs.
184.108.40.206.2 Alkaline Pretreatment
Pretreatment with ammonia solution: This is an effective treatment for lignocellulose. An ammonia solution is highly selective in reactions with lignin in comparison to carbohydrates. One of the reactions in an ammonia solution with lignin is the breakdown of the C-O-lignin as well as the ether and ester bonds in the lignin complex (Binod et al. 2010). However, this solution is an environmentally-polluting compound and a corrosive chemical.
Pretreatment with calcium and sodium hydroxide: Lime (Ca(OH)2) and sodium hydroxide (NaOH) are commonly used for the pretreatment process. During the process. Salts can be formed and incorporated into materials (González et al. 1986). The condition of this process is quite simple but the reaction time is relatively long. This pretreatment results in high solubility of lignin, especially for materials with low lignin content such as softwoods and weeds. The addition of air or oxygen during the pretreatment process can improve the efficiency of the lignin decomposition (Chang and Holtzapple 2000).
220.127.116.11 Biological Pretreatment
Biological pretreatment of feedstock for AD has attracted interest because it requires less energy input as compared with physical pretreatment and is less costly than chemical pretreatment, which costs more because of the expensive chemicals required. Hence, the biological route seems to be the most promising because it is an eco-friendly process and there is no inhibition during the process (Liu et al. 2014).
Rice straw is one of the most important biomass energy sources, largely because of its abundance. According to the Vietnam Statistical Yearbook (2018), around 23.63 million tons of rice straw are produced annually in the Mekong Delta, but more than 80% of it is burned on-site (Nguyen and Tran 2015). Typically, rice straw has a complex polymer crystal structure that is formed by the physical and chemical bonds among the cellulose, hemicellulose, and lignin components, which renders it difficult for anaerobic bacteria to utilize these components for biogas production (Sun et al. 2015). This becomes a major limitation to rice straw’s efficient utilization.
Generally, methane yields from agricultural biomass are lower compared to conventional substrates, but agricultural biomass is an inexpensive option. Biological pretreatment methods can reduce anaerobic digestion duration, enhance feedstock digestibility, and increase gas production rate. This is because the lignocellulosic components of the straw are degraded into simple substances and made easy to digest in AD, especially when using microorganisms with strong lignocellulose degradation ability. The key to the success of biological pretreatment is to find microorganisms that have exceptional lignin degradation ability and to determine the optimum digestion conditions for these microorganisms. Examples of biological pretreatment methods are: microaerobic treatments, ensiling or composting, separation of digestion stages, and fungi pretreatments.
Biological pretreatment of rice straw using fungi is a comparatively eco-friendly approach of enhancing degradability when compared with chemical pretreatment, which requires expensive chemicals, high energy inputs, and toxic substance removal, (Carrere et al. 2016). Several fungi species are used for pretreatment of lignocellulosic biomass for anaerobic digestion and most of them are the white-rot fungus (Ceriporiopsis subvermispora). A study by Zhao et al. (2014a, b) using white-rot fungus as the pretreatment agent increased methane yield by 5–15% as compared with untreated biomass.
Biological pretreatment is normally done by soaking the straw in a natural microbial solution obtained from the effluent of an anaerobic digester, anoxic sediment from ponds or lakes, and wastewater. One study found that rice straw pretreated by soaking in anoxic sediment and digester effluent for 5 days produced 79–85% more biogas volume than straw soaked in tap water (Tran et al. 2017). Compared with untreated substrates, pretreatment using microbiological action increases the degradability of substrates. Yadav et al. (2019) verified that optimal conditions for the biological treatment of lignocellulose biomass of wheat straw by Chaetomium globosporum was found to be 36 °C, 31 days, and 81% moisture, resulting in a 2.9-fold increase in reducing sugar, 48% removal of lignin, and 31% increase in biogas yield.
Similarly, a study by Shen et al. (2018) on the effect of organic loading rate on anaerobic codigestion of rice straw and pig manure showed that after biological pretreatment, the substrate was optimally fermented at an organic loading rate of 2.5 kg COD m−3 day−1. This pretreatment achieved the optimum volumetric methane production rate of 640 L CH4 m−3 day−1, and a methane yield of 456 L CH4 kg−1 COD removed, which were 62.4 and 37.8% higher than those of the control under the same organic loading rate.
Using aerobic and anaerobic fungi as pretreatment agents resulted in increased biodegradability of rice straw (Ghosh and Bhattacharyya 1999; Cann et al. 1994). Methane production from rice straw AD was increased 31–46% when pretreating it with white-rot fungus and brown-rot fungus (Polyporus ostreiformis using a straw-to-fungi ratio of 14:1 and then digested in batch reactors at 30 °C after a 3-week incubation period. Cann et al. (1994) reported that the anaerobic fungi from the rumen consistently increased the digestibility of rice straw when compared with fermenters where the fungi were inhibited. Haruta et al. (2002) also presented the enhancements by a microbial community formed by mixing rice straw, chicken feces, pig feces, cattle feces, and sugarcane dregs. This degraded 60% of the rice straw within 4 days.
Momayez et al. (2018) presented an investigation using effluent of biogas digestate to pretreat rice straw. The straw was pretreated at different temperatures (130, 60, and 190 °C) at different pretreatment times (30 and 60 min). The pretreated straw was subjected to different processes, including liquid anaerobic digestion (L-AD) and dry anaerobic digestion (D-AD). The highest methane yields were obtained through L-AD and D-AD of straw pretreated at 190 °C and 30 min, resulting in 24 and 26% increases in methane produced as compared with L-AD and D-AD using untreated straw. Mustafa et al. (2016) showed that rice straw pretreated with fungi (Pleurotus ostreatus and Trichoderma reesei) and used as feedstock for AD improved the methane yield by 120 and 78.3%, respectively, as compared with untreated straw. These points of view show that biological pretreatment studies have a huge potential, considering that there were only a few fungi species that were studied. There are still millions of fungi species that are yet to be studied.
5.3.2 Current Practices of Rice Straw AD
In current models, the rice straw floats on the surface of the substrate and gets stuck. Scientists at Can Tho University are testing a new HDPE model, which could prevent the floating straw by adjusting the inlet and outlet balance level. This upgrade could also improve the short retention time of the straw as well.
18.104.22.168 Rice Straw Batch AD
Biogas yield was in the range of 211–779 L kg−1 ODM. This experiment illustrated the following advantages of the IRRI super-bag AD: (1) the capital requirement is low with bags costing US$ 3 each, (2) floating rice straw is avoided; (3) it is portable; and (4) the digester contents are easy to unload after digestion is finished.
22.214.171.124 Two-Stage AD
Digestion temperature is maintained at from 35 to 55 °C;
Feedstock: chopped or sheared rice straw mixed with animal manure based on the ratio of 75 and 25% of organic dry matter, respectively;
Outputs: biogas for generating heat or power and digestate to produce solid and liquid fertilizer.
5.4 Utilization of Bioslurry
Applications of bioslurry on crops have been investigated since the 1940s. Gurung (1997) showed that bioslurry had a better effect on crops compared to farmyard manure. This study also revealed that AD converts 25–30% of the organic part of fecal matter into biogas, while 70–75% goes to the effluent or bioslurry.
Nutrients (N-P-K) and micronutrients (zinc, iron, manganese, and copper) in bioslurry are needed for plant growth (Tripathi 1993 as cited by Gurung 1997). Bunyeth and Preston (2004) and Sophea and Preston (2001) reported that water spinach yield responded linearly to increasing levels of nitrogen in bioslurry with pig manure. The yield of leaf mustard fertilized by the codigester effluent was 2.2 times higher compared to the inorganic fertilizer treatment. In addition to increasing the yield, the effluent can help retain more nutrients in the soil layer, accelerate flower formation, and shorten cultivation time (Nguyen et al. 2015).
Using bioslurry for fisheries was also reported in several studies. Kaur et al. (1987) presented that the growth rate of carp was 3.5 times higher in the bioslurry tank than in the control tank with raw cow dung. Increases in fish growth rate using biogas effluent were also revealed by Balasubramanian and Bai (1994) and Sophin and Preston (2001).
5.5 Conclusions and Recommendations
This review of the AD process, including AD systems from different countries, provides an overview of the current practices and trends. It should be noted that there is no such thing as a best digester design as the technology is often highly localized. An AD technology may work well in one country but not in another because of different conditions.
With regards to the use of rice straw for AD, studies have shown that it is a feasible and sustainable technology, especially when rice straw is codigested with other biological wastes such as animal manure. Using the optimum pretreatment, conditions, and operational parameters and a mixture proportion with other substrates, methane production from AD of rice straw can be maximized. In addition, the digestate byproduct can be processed to produce biofertilizer.
To increase the adoption of AD technologies, awareness of the technology must be increased and the rice straw and biogas value chain must be upgraded. AD technologies that are easy to adopt, such as the use of hermetic bags for AD, can be useful to farmers. Subsequent increase in demand for biogas and biofertilizers can be supported by larger scale technologies such as the two-stage AD.
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