Abstract
To overcome the limitations of the traditional 2D cell culture platform, significant advances have been achieved for the emergence and rapid growth of 3D in vitro culture systems that can model the fundamental biology and pharmaceutics of tissues and organs. This chapter focuses on the most prevalent technologies that have been widely used for establishing the in vitro tissue/organ models, including transwell systems, cell spheroids/sheets, organoids, and microfluidic tissue/organ-on-a-chip. For these techniques, the working principle are individually introduced, followed by the discussion of their advantages and limitations. In addition, application cases of modeling the pathophysiology of tissues/organs are described for understanding of their characteristics and potentials.
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Shi Y, Fan X, Meng W, Deng H, Zhang N, An Z. Engagement of immune effector cells by trastuzumab induces HER2/ERBB2 downregulation in cancer cells through STAT1 activation. Breast Cancer Res. 2014;16(2):R33.
Wang Y, Wang N, Cai B, Wang G-y, Li J, Piao X-x. In vitro model of the blood-brain barrier established by co-culture of primary cerebral microvascular endothelial and astrocyte cells. Neural Regen Res. 2015;10(12):2011.
Lee Y, Dizzell S, Leung V, Nazli A, Zahoor M, Fichorova R, Kaushic C. Effects of female sex hormones on susceptibility to HSV-2 in vaginal cells grown in air-liquid interface. Viruses. 2016;8(9):241.
Lin H, Li H, Cho HJ, Bian S, Roh HJ, Lee MK, Kim JS, Chung SJ, Shim CK, Kim DD. Air‐liquid interface (ALI) culture of human bronchial epithelial cell monolayers as an in vitro model for airway drug transport studies. J Pharm Sci. 2007;96(2):341–50.
Li L, Fukunaga-Kalabis M, Herlyn M. The three-dimensional human skin reconstruct model: a tool to study normal skin and melanoma progression. J Vis Exp. 2011;(54):e2937.
Hoarau-Véchot J, Rafii A, Touboul C, Pasquier J. Halfway between 2D and animal models: are 3D cultures the ideal tool to study cancer-microenvironment interactions? Int J Mol Sci. 2018;19(1):181.
Kelm JM, Sanchez-Bustamante CD, Ehler E, Hoerstrup SP, Djonov V, Ittner L, Fussenegger M. VEGF profiling and angiogenesis in human microtissues. J Biotechnol. 2005;118(2):213–29.
Li M, Ma J, Gao Y, Yang L. Cell sheet technology: a promising strategy in regenerative medicine. Cytotherapy. 2019;21:3.
Matsuda N, Shimizu T, Yamato M, Okano T. Tissue engineering based on cell sheet technology. Adv Mater. 2007;19(20):3089–99.
Sasagawa T, Shimizu T, Sekiya S, Haraguchi Y, Yamato M, Sawa Y, Okano T. Design of prevascularized three-dimensional cell-dense tissues using a cell sheet stacking manipulation technology. Biomaterials. 2010;31(7):1646–54.
Haraguchi Y, Shimizu T, Yamato M, Okano T. Scaffold-free tissue engineering using cell sheet technology. RSC Adv. 2012;2(6):2184–90.
Fatehullah A, Tan SH, Barker N. Organoids as an in vitro model of human development and disease. Nat Cell Biol. 2016;18(3):246.
Clevers H. Modeling development and disease with organoids. Cell. 2016;165(7):1586–97.
Yin X, Mead BE, Safaee H, Langer R, Karp JM, Levy O. Engineering stem cell organoids. Cell Stem Cell. 2016;18(1):25–38.
Lancaster MA, Knoblich JA. Generation of cerebral organoids from human pluripotent stem cells. Nat Protoc. 2014;9(10):2329.
Drost J, Clevers H. Organoids in cancer research. Nat Rev Cancer. 2018;18(7):407.
Ciancanelli MJ, Huang SX, Luthra P, Garner H, Itan Y, Volpi S, Lafaille FG, Trouillet C, Schmolke M, Albrecht RA. Life-threatening influenza and impaired interferon amplification in human IRF7 deficiency. Science. 2015;348(6233):448–53.
Takasato M, Pei XE, Chiu HS, Maier B, Baillie GJ, Ferguson C, Parton RG, Wolvetang EJ, Roost MS, de Sousa Lopes SMC. Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. Nature. 2015;526(7574):564.
Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–8.
Li X, Tian T. Recent advances in an organ-on-a-chip: biomarker analysis and applications. Anal Methods. 2018;10(26):3122–30.
Konar D, Devarasetty M, Yildiz DV, Atala A, Murphy SV. Lung-on-a-chip technologies for disease modeling and drug development: supplementary issue: image and video acquisition and processing for clinical applications. Biomed Eng Comput Biol. 2016;7:BECB.S34252.
Ma X, Liu J, Zhu W, Tang M, Lawrence N, Yu C, Gou M, Chen S. 3D bioprinting of functional tissue models for personalized drug screening and in vitro disease modeling. Adv Drug Deliv Rev. 2018;132:235–51.
Murphy SV, Atala A. 3D bioprinting of tissues and organs. Nat Biotechnol. 2014;32(8):773.
Mandrycky C, Wang Z, Kim K, Kim D-H. 3D bioprinting for engineering complex tissues. Biotechnol Adv. 2016;34(4):422–34.
Homan KA, Kolesky DB, Skylar-Scott MA, Herrmann J, Obuobi H, Moisan A, Lewis JA. Bioprinting of 3D convoluted renal proximal tubules on perfusable chips. Sci Rep. 2016;6:34845.
Gao G, Soo Kim B, Jang J, Cho DW. Recent strategies in extrusion-based three-dimensional cell printing toward organ biofabrication. ACS Biomater Sci Eng. 2019;5:1150.
Datta P, Ayan B, Ozbolat IT. Bioprinting for vascular and vascularized tissue biofabrication. Acta Biomater. 2017;51:1–20.
Marcos R, Monteiro RA, Rocha E. Design‐based stereological estimation of hepatocyte number, by combining the smooth optical fractionator and immunocytochemistry with anti‐carcinoembryonic antigen polyclonal antibodies. Liver Int. 2006;26(1):116–24.
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Cho, DW., Kim, B.S., Jang, J., Gao, G., Han, W., Singh, N.K. (2019). Prevalent Technologies for In Vitro Tissue/Organ Modeling. In: 3D Bioprinting. Springer, Cham. https://doi.org/10.1007/978-3-030-32222-9_3
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DOI: https://doi.org/10.1007/978-3-030-32222-9_3
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