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Influence of Mutual Rotation of Polarizing Filters on Light Intensity Measured with Collagen Fibres

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VipIMAGE 2019 (VipIMAGE 2019)

Part of the book series: Lecture Notes in Computational Vision and Biomechanics ((LNCVB,volume 34))

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Abstract

The most common technique for observation of collagen fibres and evaluation of their directionality is polarized light microscopy. In the microscope, there are two polarizing filters, polarizer and analyzer, that can rotate individually. The default position of the polarizer is perpendicular to the analyzer. When the light passes through a birefringent specimen, the plane of the polarized light is rotated. This leads to a \(90^{\circ }\) periodic change in light intensity passing through the sample and both filters. If the orientation of the polarizer differs from the perpendicular direction to the analyzer, the period of light intensity changes to \(180^{\circ }\); one of the original maxima increases and the other decreases. This change is very useful for the determination of directions of collagen fibres by an automatic algorithm.

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Acknowledgements

This work was supported by Czech Science Foundation project No. 18-13663S.

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Authors and Affiliations

  1. Institute of Solid Mechanics, Mechatronics and Biomechanics, Brno University of Technology, Brno, Czech Republic

    Michaela Turčanová & Jiří Burša

  2. Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic

    Martin Hrtoň

  3. Institute of Physical Engineering, Brno University of Technology, Brno, Czech Republic

    Petr Dvořák

Authors
  1. Michaela Turčanová
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  2. Martin Hrtoň
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  3. Petr Dvořák
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  4. Jiří Burša
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Corresponding author

Correspondence to Michaela Turčanová .

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Editors and Affiliations

  1. Faculdade de Engenharia, Universidade do Porto, Porto, Portugal

    João Manuel R. S. Tavares

  2. Faculdade de Engenharia, Universidade do Porto, Porto, Portugal

    Renato Manuel Natal Jorge

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Turčanová, M., Hrtoň, M., Dvořák, P., Burša, J. (2019). Influence of Mutual Rotation of Polarizing Filters on Light Intensity Measured with Collagen Fibres. In: Tavares, J., Natal Jorge, R. (eds) VipIMAGE 2019. VipIMAGE 2019. Lecture Notes in Computational Vision and Biomechanics, vol 34. Springer, Cham. https://doi.org/10.1007/978-3-030-32040-9_29

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