Abstract
Genome minimization ultimately leads to the smallest genome sustaining life of a given cell; however, growth of this cell may be very slow and may require multiple supplements, e.g., to overcome amino acid auxotrophies. By contrast, genome reduction of industrially relevant bacteria such as Corynebacterium glutamicum does not aim at generating minimal cells. Rather chassis cells are developed that are as fit as the wild type with respect to a target function, for example, growth of C. glutamicum in glucose minimal medium. Thus, a balance between reducing the burden of expressed genes and maintaining fast growth with glucose without the requirement for supplements such as amino acids is required. Here, the application of this concept to C. glutamicum is discussed. Moreover, an outlook on how the advent of genome editing by CRISPR-Cas9 or CRISPR-Cas12a(Cpf1) impacts genome reduction and how highly parallel genome editing must be met by highly parallel strain characterization is presented. Finally, metabolic engineering approaches for the overproduction of amino acids, organic acids, terpenoids, and diamines making use of genome-reduced C. glutamicum strains are detailed.
Google Scholar Profile: XBj3q30AAAAJ; Researcher ID: M-5940-2016; Scopus Author ID: 6701420809
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Acknowledgment
VFW gratefully acknowledges support by ERACoBiotech grant INDIE (BMEL 22023517), by the Indo-German project BIOCON (BMBF 01DQ17009) and by funding from the state of North Rhine-Westphalia (NRW) and the “European Regional Development Fund (EFRE)”, Project “ClusterIndustrial Biotechnology (CLIB) Kompetenzzentrum Biotechnologie (CKB)” (34.EFRE-0300095/1703FI04).
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Wendisch, V.F. (2020). Genome-Reduced Corynebacterium glutamicum Fit for Biotechnological Applications. In: Lara, A., Gosset, G. (eds) Minimal Cells: Design, Construction, Biotechnological Applications. Springer, Cham. https://doi.org/10.1007/978-3-030-31897-0_4
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