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Effect of Light Stimulation on a Thermo-Cellulolytic Bacterial Consortium Used for the Degradation of Cellulose of Green Coconut Shells

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Emerging Research in Science and Engineering Based on Advanced Experimental and Computational Strategies

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Abstract

The green coconut business is responsible for the generation of large amounts of waste. Sustainable development requires the transformation of such waste into value-added biotechnological products. The aim of this study was to optimize, by photostimulation, the hydrolysis of the coconut biomass. The thermo-cellulolytic consortium was collected from a composting pile, subjected to nutritional stress and irradiated either by Laser (λ660 ηm) or LED (λ632 ± 2 ηm). Microbial quantification after irradiation showed a significant stimulatory biological response. Despite cultures irradiated by LED significantly differed from the Laser-irradiated ones (p < 0.0001) both were significantly different from the control (p < 0.0001). The microbial consortium irradiated either by Laser or LED light showed the increase of RNA production and consequently protein synthesis causing anticipation and increase of the RBBR catabolism. The generation of products by cellulose hydrolysis (TRS and glucose) was significantly higher in the photostimulated groups, being the most effective catabolism observed within the first 48-h in the LED group and, after 144-h, in the Laser group. Photostimulation, especially by LED, might be considered as a booster of the bioprocess at low cost.

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Authors and Affiliations

  1. Laboratory of Biotechnology and Ecology of Micro-organisms, Institute of Health Science, Federal University of Bahia, Reitor Miguel Calmon Ave, S/N, Salvador, BA, 40110-100, Brazil

    Pedro Jorge Louro Crugeira, F. A. Chinalia, J. B. T. L. Matos & P. F. Almeida

  2. Department of Saude, Feira de Santana State University, Feira de Santa, BA, 44036-900, Brazil

    H. N. Brandão

  3. Center of Biophotonics, Federal University of Bahia, 62, Araujo Pinho Ave, Canela, Salvador, BA, 40110-150, Brazil

    A. L. B. Pinheiro

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  1. Pedro Jorge Louro Crugeira
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  2. F. A. Chinalia
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  3. H. N. Brandão
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  4. J. B. T. L. Matos
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  5. A. L. B. Pinheiro
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Correspondence to Pedro Jorge Louro Crugeira .

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  1. Dept de Química, Univ Tecno Federal do Parana, Londrina, Brazil

    Felipe de Almeida La Porta

  2. Centro Brasileiro de Pesquisas Físicas, Rio de Janeiro, Brazil

    Carlton A. Taft

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Crugeira, P.J.L., Chinalia, F.A., Brandão, H.N., Matos, J.B.T.L., Pinheiro, A.L.B., Almeida, P.F. (2020). Effect of Light Stimulation on a Thermo-Cellulolytic Bacterial Consortium Used for the Degradation of Cellulose of Green Coconut Shells. In: La Porta, F., Taft, C. (eds) Emerging Research in Science and Engineering Based on Advanced Experimental and Computational Strategies. Engineering Materials. Springer, Cham. https://doi.org/10.1007/978-3-030-31403-3_5

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  • DOI: https://doi.org/10.1007/978-3-030-31403-3_5

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