Abstract
The regenerative medicine field has promising solutions to overcome existing clinical challenges in the repair or regrowth of injured tissues. To date, an enormous progress has been made in developing numerous strategies for enhanced regeneration. The nanofibers fabricated by electrospinning offer excellent characteristics mimicking the extracellular matrix that support cell adhesion, migration, and differentiation, which are responsible for the regeneration of tissues. Furthermore, due to their ease of production, cost-effectiveness, and ability to have various compositions and different morphologies, the electrospun nanofibers have been extensively explored for their possibilities in the regeneration of various tissues. In the present chapter, we summarize the examples of electrospun nanofibers fabricated for the regeneration of dermal, neural, and orthopedic tissues.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Aravindan, V., Sundaramurthy, J., Suresh Kumar, P., Lee, Y. S., Ramakrishna, S., & Madhavi, S. (2015). Electrospun nanofibers: A prospective electro-active material for constructing high performance Li-ion batteries. Chemical Communications, 51, 2225–2234. https://doi.org/10.1039/c4cc07824a
Arora, P., Sindhu, A., Dilbaghi, N., Chaudhury, A., Rajakumar, G., & Rahuman, A. A. (2012). Nano-regenerative medicine towards clinical outcome of stem cell and tissue engineering in humans. Journal of Cellular and Molecular Medicine, 16(9), 1991–2000. https://doi.org/10.1111/j.1582-4934.2012.01534.x
Asencio, I. O., Mittar, S., Sherborne, C., Raza, A., Claeyssens, F., & MacNeil, S. (2018). A methodology for the production of microfabricated electrospun membranes for the creation of new skin regeneration models. Journal of Tissue Engineering, 9, 1–8. https://doi.org/10.1177/2041731418799851
Atala, A. (2012). Regenerative medicine strategies. Journal of Pediatric Surgery, 47, 17–28. https://doi.org/10.1016/j.jpedsurg.2011.10.013
Babitha, S., Rachita, L., Karthikeyan, K., Shoba, E., Janani, I., Poornima, B., & Purna Sai, K. (2017). Electrospun protein nanofibers in healthcare: A review. International Journal of Pharmaceutics, 523, 52–90. https://doi.org/10.1016/j.ijpharm.2017.03.013
Baker, B. M., Handorf, A. M., Ionescu, L. C., Li, W. J., & Mauck, R. L. (2009). New directions in nanofibrous scaffolds for soft tissue engineering and regeneration. Expert Review of Medical Devices, 6, 515–532. https://doi.org/10.1586/erd.09.39
Biazar, E., & Keshel, S. H. (2014). Unrestricted somatic stem cells loaded in nanofibrous scaffolds as potential candidate for skin regeneration. International Journal of Polymeric Materials and Polymeric Biomaterials, 63(14), 741–752. https://doi.org/10.1080/00914037.2013.879447
Boda, S. K., Almoshari, Y., Wang, H., Wang, X., Reinhardt, R. A., Duan, B., … Xie, J. (2019). Mineralized nanofiber segments coupled with calcium-binding BMP-2 peptides for alveolar bone regeneration. Acta Biomaterialia, 85, 282–293. https://doi.org/10.1016/j.actbio.2018.12.051
Colombo, F., Sampogna, G., Cocozza, G., Guraya, S., & Forgione, A. (2016). Regenerative medicine: Clinical applications and future perspectives. Journal of Microscopy and Ultrastructure, 5(1), 1. https://doi.org/10.1016/j.jmau.2016.05.002
Corsi, K. A., Schwarz, E. M., Mooney, D. J., & Huard, J. (2007). Regenerative medicine in orthopaedic surgery. Journal of Orthopaedic Research, 25, 1261–1268. https://doi.org/10.1002/jor.20432
Cui, H., Nowicki, M., Fisher, J. P., & Zhang, L. G. (2017). 3D bioprinting for organ regeneration. Advanced Healthcare Materials, 6, 1601118. https://doi.org/10.1002/adhm.201601118
Deepthi, S., Nivedhitha Sundaram, M., Deepti Kadavan, J., & Jayakumar, R. (2016). Layered chitosan-collagen hydrogel/aligned PLLA nanofiber construct for flexor tendon regeneration. Carbohydrate Polymers, 153, 492–500. https://doi.org/10.1016/j.carbpol.2016.07.124
Dias, J. R., Baptista-Silva, S., de Oliveira, C. M. T., Sousa, A., Oliveira, A. L., Bártolo, P. J., & Granja, P. L. (2017). In situ crosslinked electrospun gelatin nanofibers for skin regeneration. European Polymer Journal, 95, 161–173. https://doi.org/10.1016/j.eurpolymj.2017.08.015
Ding, B., Wang, X., & Yu, J. (2019). Electrospinning: Nanofabrication and applications. https://doi.org/10.1016/c2016-0-01374-8
Ding, Y., Hou, H., Zhao, Y., Zhu, Z., & Fong, H. (2016). Electrospun polyimide nanofibers and their applications. Progress in Polymer Science, 61, 67–103. https://doi.org/10.1016/j.progpolymsci.2016.06.006
Dzobo, K., Thomford, N. E., Senthebane, D. A., Shipanga, H., Rowe, A., Dandara, C., … Motaung, K. S. C. M. (2018). Advances in regenerative medicine and tissue engineering: Innovation and transformation of medicine. Stem Cells International, 2018, 2495848. https://doi.org/10.1155/2018/2495848
Fee, T., Surianarayanan, S., Downs, C., Zhou, Y., & Berry, J. (2016). Nanofiber alignment regulates NIH3T3 cell orientation and cytoskeletal gene expression on electrospun PCL+gelatin nanofibers. PLoS One, 11(5), e0154806. https://doi.org/10.1371/journal.pone.0154806
Fernandes, H., Moroni, L., Van Blitterswijk, C., & De Boer, J. (2009). Extracellular matrix and tissue engineering applications. Journal of Materials Chemistry, 19(31), 5474–5484. https://doi.org/10.1039/b822177d
Gandhimathi, C., Venugopal, J. R., Bhaarathy, V., & Ramakrishna, Kumar. (2014). Biocomposite nanofibrous strategies for the controlled release of biomolecules for skin tissue regeneration. International Journal of Nanomedicine, 9, 4709. https://doi.org/10.2147/ijn.s65335
Grafahrend, D., Heffels, K. H., Möller, M., Klee, D., & Groll, J. (2010). Electrospun, biofunctionalized fibers as tailored in vitro substrates for keratinocyte cell culture. Macromolecular Bioscience, 10(9), 1022–1027. https://doi.org/10.1002/mabi.201000068
Gu, W., Wu, C., Chen, J., & Xiao, Y. (2013). Nanotechnology in the targeted drug delivery for bone diseases and bone regeneration. International Journal of Nanomedicine, 8, 2305–2317. https://doi.org/10.2147/IJN.S44393
Gui, X., Hu, J., & Han, Y. (2019). Random and aligned electrospun gelatin nanofiber mats for human mesenchymal stem cells. Materials Research Innovations, 23(4), 208–215. https://doi.org/10.1080/14328917.2018.1428073
Hakimi, O., Mouthuy, P. A., Zargar, N., Lostis, E., Morrey, M., & Carr, A. (2015). A layered electrospun and woven surgical scaffold to enhance endogenous tendon repair. Acta Biomaterialia, 26, 124–135. https://doi.org/10.1016/j.actbio.2015.08.007
Huang, C. Y., Hu, K. H., & Wei, Z. H. (2016). Comparison of cell behavior on PVA/PVA-gelatin electrospun nanofibers with random and aligned configuration. Scientific Reports, 6, 37960. https://doi.org/10.1038/srep37960
Hynes, R. O. (2009). The extracellular matrix: Not just pretty fibrils. Science, 326, 1216–1219. https://doi.org/10.1126/science.1176009
Jahani, H., Kaviani, S., Hassanpour-Ezatti, M., Soleimani, M., Kaviani, Z., & Zonoubi, Z. (2012). The effect of aligned and random electrospun fibrous scaffolds on rat mesenchymal stem cell proliferation. Cell Journal, 14(1), 31–38.
Jeong, L., Yeo, I. S., Kim, H. N., Yoon, Y. I., Jang, D. H., Jung, S. Y., … Park, W. H. (2009). Plasma-treated silk fibroin nanofibers for skin regeneration. International Journal of Biological Macromolecules, 44(3), 222–228. https://doi.org/10.1016/j.ijbiomac.2008.12.008
Jin, L., Xu, Q., Li, C., Huang, J., Zhang, Y., Wu, D., & Wang, Z. (2017). Engineering 3D aligned nanofibers for regulation of cell growth behavior. Macromolecular Materials and Engineering, 302(4), 1600448. https://doi.org/10.1002/mame.201600448
Kim, E. S., Ahn, E. H., Dvir, T., & Kim, D. H. (2014). Emerging nanotechnology approaches in tissue engineering and regenerative medicine. International Journal of Nanomedicine, 9, 1–5. https://doi.org/10.2147/IJN.S61212
Kim, J. I., Hwang, T. I., Aguilar, L. E., Park, C. H., & Kim, C. S. (2016). A controlled design of aligned and random nanofibers for 3D bi-functionalized nerve conduits fabricated via a novel electrospinning set-up. Scientific Reports, 6, 23761. https://doi.org/10.1038/srep23761
Kim, Y., Ko, H., Kwon, I. K., & Shin, K. (2016). Extracellular matrix revisited: Roles in tissue engineering. International Neurourology Journal, 20, S23–S29. https://doi.org/10.5213/inj.1632600.318
Kny, E., Ghosal, K., & Thomas, S. (2018). Electrospinning: From basic research to commercialization (Soft matter series) (Vol. 7). London: The Royal Society of Chemistry. https://doi.org/10.1039/9781788010580-FP007
Kular, J. K., Basu, S., & Sharma, R. I. (2014). The extracellular matrix: Structure, composition, age-related differences, tools for analysis and applications for tissue engineering. Journal of Tissue Engineering, 5, 2041731414557112. https://doi.org/10.1177/2041731414557112
Kumar, R., Griffin, M., & Butler, P. E. (2017). A review of current regenerative medicine strategies that utilize nanotechnology to treat cartilage damage. The Open Orthopaedics Journal, 10(1), 862–876. https://doi.org/10.2174/1874325001610010862
Kusindarta, D. L., & Wihadmadyatami, H. (2018). The role of extracellular matrix in tissue regeneration. In H. H. E.-S. Kaoud (Ed.), Tissue regeneration. Croatia: Intech. https://doi.org/10.5772/intechopen.75728
Lee, P., Tran, K., Chang, W., Shelke, N. B., Kumbar, S. G., & Yu, X. (2014). Influence of chondroitin sulfate and hyaluronic acid presence in nanofibers and its alignment on the bone marrow stromal cells: Cartilage regeneration. Journal of Biomedical Nanotechnology, 10(8), 1469–1479.
Li, Z., & Wang, C. (2013). One dimensional nanostructures: Electrospinning technique and unique nanofibers. Berlin: Springer. https://doi.org/10.1007/978-3-642-36427-3
Lin, T., & Fang, J. (2017). Fundamentals of electrospinning & electrospun nanofibers. Pennsylvania, PA: DEStech Publications Inc.
Lipner, J., Shen, H., Cavinatto, L., Liu, W., Havlioglu, N., Xia, Y., … Thomopoulos, S. (2015). In vivo evaluation of adipose-derived stromal cells delivered with a nanofiber scaffold for tendon-to-bone repair. Tissue Engineering Part A, 21(21–22), 2766–2774. https://doi.org/10.1089/ten.tea.2015.0101
Liu, W., Thomopoulos, S., & Xia, Y. (2012). Electrospun nanofibers for regenerative medicine. Advanced Healthcare Materials, 1(1), 10–25. https://doi.org/10.1002/adhm.201100021
López-Cebral, R., Silva-Correia, J., Reis, R. L., Silva, T. H., & Oliveira, J. M. (2017). Peripheral nerve injury: Current challenges, conventional treatment approaches, and new trends in biomaterials-based regenerative strategies. ACS Biomaterials Science and Engineering, 3, 3098–3122. https://doi.org/10.1021/acsbiomaterials.7b00655
Ma, B., Xie, J., Jiang, J., Shuler, F. D., & Bartlett, D. E. (2013). Rational design of nanofiber scaffolds for orthopedic tissue repair and regeneration. Nanomedicine, 8, 1459–1481. https://doi.org/10.2217/nnm.13.132
Ma, G., Yang, D., Wang, K., Han, J., Ding, S., Song, G., & Nie, J. (2010). Organic-soluble chitosan/polyhydroxybutyrate ultrafine fibers as skin regeneration prepared by electrospinning. Journal of Applied Polymer Science, 118(6), 3619–3624. https://doi.org/10.1002/app.32671
Mackiewicz, Z., Konttinen, Y., Kaivosoja, E., Stegajev, V., Wagner, H., Levón, J., & Tiainen, V. (2016). Extracellular matrix and tissue regeneration. In G. Steinhoff (Ed.), Regenerative medicine—From protocol to patient. Cham: Springer.
MacLean, S., Khan, W. S., Malik, A. A., Snow, M., & Anand, S. (2012). Tendon regeneration and repair with stem cells. Stem Cells International, 2012, 316281. https://doi.org/10.1155/2012/316281
Manning, C. N., Schwartz, A. G., Liu, W., Xie, J., Havlioglu, N., Sakiyama-Elbert, S. E., … Thomopoulos, S. (2013). Controlled delivery of mesenchymal stem cells and growth factors using a nanofiber scaffold for tendon repair. Acta Biomaterialia, 9(6), 6905–6914. https://doi.org/10.1016/j.actbio.2013.02.008
McLane, J. S., Schaub, N. J., Gilbert, R. J., & Ligon, L. A. (2013). Electrospun nanofiber scaffolds for investigating cell–matrix adhesion. In A. Coutts (Ed.), Adhesion protein protocols. Methods in molecular biology (Methods and protocols). Totowa, NJ: Humana Press.
Miao, S., Castro, N., Nowicki, M., Xia, L., Cui, H., Zhou, X., … Zhang, L. G. (2017). 4D printing of polymeric materials for tissue and organ regeneration. Materials Today, 20, 577–591. https://doi.org/10.1016/j.mattod.2017.06.005
Mitchell, G. (2014). Electrospinning: Principles, practice and possibilities. The Royal Society of Chemistry: Cambridge, UK.
Mohabatpour, F., Karkhaneh, A., & Sharifi, A. M. (2016). A hydrogel/fiber composite scaffold for chondrocyte encapsulation in cartilage tissue regeneration. RSC Advances, 6(86), 83135–83145. https://doi.org/10.1039/c6ra15592h
Nam, Y., Rim, Y. A., Lee, J., & Ju, J. H. (2018). Current therapeutic strategies for stem cell-based cartilage regeneration. Stem Cells International, 2018, 8490489. https://doi.org/10.1155/2018/8490489
Narayanan, N., Jiang, C., Uzunalli, G., Thankappan, S. K., Laurencin, C. T., & Deng, M. (2016). Polymeric electrospinning for musculoskeletal regenerative engineering. Regenerative Engineering and Translational Medicine, 2, 69–84. https://doi.org/10.1007/s40883-016-0013-8
Nedjari, S., Awaja, F., & Altankov, G. (2017). Three dimensional honeycomb patterned fibrinogen based nanofibers induce substantial osteogenic response of mesenchymal stem cells. Scientific Reports, 7(1), 15947. https://doi.org/10.1038/s41598-017-15956-8
Noh, H. K., Lee, S. W., Kim, J. M., Oh, J. E., Kim, K. H., Chung, C. P., … Min, B. M. (2006). Electrospinning of chitin nanofibers: Degradation behavior and cellular response to normal human keratinocytes and fibroblasts. Biomaterials, 27(21), 3934–3944. https://doi.org/10.1016/j.biomaterials.2006.03.016
Norouzi, M., Boroujeni, S. M., Omidvarkordshouli, N., & Soleimani, M. (2015). Advances in skin regeneration: Application of electrospun scaffolds. Advanced Healthcare Materials, 4, 1114–1133. https://doi.org/10.1002/adhm.201500001
Pak, J., Lee, J. H., Pak, N., Pak, Y., Park, K. S., Jeon, J. H., … Lee, S. H. (2018). Cartilage regeneration in humans with adipose tissue-derived stem cells and adipose stromal vascular fraction cells: Updated status. International Journal of Molecular Sciences, 19, E2146. https://doi.org/10.3390/ijms19072146
Pal, P., Srivas, P. K., Dadhich, P., Das, B., Maulik, D., & Dhara, S. (2017). Nano-/microfibrous cotton-wool-like 3D scaffold with core-shell architecture by emulsion electrospinning for skin tissue regeneration. ACS Biomaterials Science and Engineering, 3(12), 3563–3575. https://doi.org/10.1021/acsbiomaterials.7b00681
Panahi-Joo, Y., Karkhaneh, A., Nourinia, A., Abd-Emami, B., Negahdari, B., Renaud, P., & Bonakdar, S. (2016). Design and fabrication of a nanofibrous polycaprolactone tubular nerve guide for peripheral nerve tissue engineering using a two-pole electrospinning system. Biomedical Materials (Bristol), 11(2), 025017. https://doi.org/10.1088/1748-6041/11/2/025017
Paredes, J. J., & Andarawis-Puri, N. (2016). Therapeutics for tendon regeneration: A multidisciplinary review of tendon research for improved healing. Annals of the New York Academy of Sciences, 1383(1), 125–138. https://doi.org/10.1111/nyas.13228
Park, K. E., Kim, B. S., Kim, M. H., You, H. K., Lee, J., & Park, W. H. (2015). Basic fibroblast growth factor-encapsulated PCL nano/microfibrous composite scaffolds for bone regeneration. Polymer, 76, 8–16. https://doi.org/10.1016/j.polymer.2015.08.024
Piai, J. F., da Silva, M. A., Martins, A., Torres, A. B., Faria, S., Reis, R. L., … Neves, N. M. (2017). Chondroitin sulfate immobilization at the surface of electrospun nanofiber meshes for cartilage tissue regeneration approaches. Applied Surface Science, 403, 112–125. https://doi.org/10.1016/j.apsusc.2016.12.135
Prabhakaran, M. P., Venugopal, J., & Ramakrishna, S. (2009). Electrospun nanostructured scaffolds for bone tissue engineering. Acta Biomaterialia, 5(8), 2884–2893. https://doi.org/10.1016/j.actbio.2009.05.007
Reinke, J. M., & Sorg, H. (2012). Wound repair and regeneration. European Surgical Research, 49(1), 35–43. https://doi.org/10.1159/000339613
Román-Doval, R., Tellez-Cruz, M. M., Rojas-Chávez, H., Cruz-Martínez, H., Carrasco-Torres, G., & Vásquez-Garzón, V. R. (2019). Enhancing electrospun scaffolds of PVP with polypyrrole/iodine for tissue engineering of skin regeneration by coating via a plasma process. Journal of Materials Science, 54(4), 3342–3353. https://doi.org/10.1007/s10853-018-3024-7
Romanovsky, A. A. (2014). Skin temperature: Its role in thermoregulation. Acta Physiologica, 210, 498–507. https://doi.org/10.1111/apha.12231
Sell, S., Barnes, C., Smith, M., McClure, M., Madurantakam, P., Grant, J., … Bowlin, G. (2007). Extracellular matrix regenerated: Tissue engineering via electrospun biomimetic nanofibers. Polymer International, 56, 1349–1360. https://doi.org/10.1002/pi.2344
Senthamizhan, A., Balusamy, B., & Uyar, T. (2016). Glucose sensors based on electrospun nanofibers: A review fiber-based platforms for bioanalytics. Analytical and Bioanalytical Chemistry, 408(5), 1285–1306. https://doi.org/10.1007/s00216-015-9152-x
Shafiee, A., Soleimani, M., Chamheidari, G. A., Seyedjafari, E., Dodel, M., Atashi, A., & Gheisari, Y. (2011). Electrospun nanofiber-based regeneration of cartilage enhanced by mesenchymal stem cells. Journal of Biomedical Materials Research—Part A, 99A(3), 467–478. https://doi.org/10.1002/jbm.a.33206
Shahriar, S., Mondal, J., Hasan, M., Revuri, V., Lee, D., & Lee, Y.-K. (2019). Electrospinning nanofibers for therapeutics delivery. Nanomaterials, 9(4), 532. https://doi.org/10.3390/nano9040532
Shan, X., Liu, C., Li, F., Ouyang, C., Gao, Q., & Zheng, K. (2015). Nanoparticles vs. nanofibers: A comparison of two drug delivery systems on assessing drug release performance in vitro. Designed Monomers and Polymers, 18(7), 678–689. https://doi.org/10.1080/15685551.2015.1070500
Sheikh, F. A., Ju, H. W., Lee, J. M., Moon, B. M., Park, H. J., Lee, O. J., … Park, C. H. (2015). 3D electrospun silk fibroin nanofibers for fabrication of artificial skin. Nanomedicine: Nanotechnology, Biology, and Medicine, 11(3), 681–691. https://doi.org/10.1016/j.nano.2014.11.007
So, K. F., & Xu, X. M. (2015). Neural regeneration. Cambridge, MA: Academic Press. https://doi.org/10.1016/C2014-0-00519-9
Sridhar, R., Lakshminarayanan, R., Madhaiyan, K., Barathi, V. A., Limh, K. H. C., & Ramakrishna, S. (2015). Electrosprayed nanoparticles and electrospun nanofibers based on natural materials: Applications in tissue regeneration, drug delivery and pharmaceuticals. Chemical Society Reviews, 44, 790–814. https://doi.org/10.1039/c4cs00226a
Stoll, E. A. (2014). Advances toward regenerative medicine in the central nervous system: Challenges in making stem cell therapy a viable clinical strategy. Molecular and Cellular Therapies, 2(1), 12. https://doi.org/10.1186/2052-8426-2-12
Tchemtchoua, V. T., Atanasova, G., Aqil, A., Filée, P., Garbacki, N., Vanhooteghem, O., … Colige, A. (2011). Development of a chitosan nanofibrillar scaffold for skin repair and regeneration. Biomacromolecules, 12(9), 3194–3204. https://doi.org/10.1021/bm200680q
Thorvaldsson, A., Stenhamre, H., Gatenholm, P., & Walkenström, P. (2008). Electrospinning of highly porous scaffolds for cartilage regeneration. Biomacromolecules, 9(3), 1044–1049. https://doi.org/10.1021/bm701225a
Tiku, M. L., & Sabaawy, H. E. (2015). Cartilage regeneration for treatment of osteoarthritis: A paradigm for nonsurgical intervention. Therapeutic Advances in Musculoskeletal Disease, 7, 76–87. https://doi.org/10.1177/1759720X15576866
Uyar, T., & Kny, E. (2017). Electrospun materials for tissue engineering and biomedical applications (1st ed.). https://doi.org/10.1016/c2015-0-05802-6
Vacanti, J. (2010). Tissue engineering and regenerative medicine: From first principles to state of the art. Journal of Pediatric Surgery, 45, 291–294. https://doi.org/10.1016/j.jpedsurg.2009.10.063
Vashisth, P., Nikhil, K., Roy, P., Pruthi, P. A., Singh, R. P., & Pruthi, V. (2016). A novel gellan-PVA nanofibrous scaffold for skin tissue regeneration: Fabrication and characterization. Carbohydrate Polymers, 136, 851–859. https://doi.org/10.1016/j.carbpol.2015.09.113
Venugopal, J., & Ramakrishna, S. (2005). Biocompatible nanofiber matrices for the engineering of a dermal substitute for skin regeneration. Tissue Engineering, 11(5–6), 847–854. https://doi.org/10.1089/ten.2005.11.847
Verma, S., Domb, A. J., & Kumar, N. (2011). Nanomaterials for regenerative medicine. Nanomedicine, 6, 157–181. https://doi.org/10.2217/nnm.10.146
Vig, K., Chaudhari, A., Tripathi, S., Dixit, S., Sahu, R., Pillai, S., … Singh, S. R. (2017). Advances in skin regeneration using tissue engineering. International Journal of Molecular Sciences, 18, 789. https://doi.org/10.3390/ijms18040789
Vijayavenkataraman, S., Yan, W. C., Lu, W. F., Wang, C. H., & Fuh, J. Y. H. (2018). 3D bioprinting of tissues and organs for regenerative medicine. Advanced Drug Delivery Reviews, 132, 296–332. https://doi.org/10.1016/j.addr.2018.07.004
Vimal, S. K., Ahamad, N., & Katti, D. S. (2016). A simple method for fabrication of electrospun fibers with controlled degree of alignment having potential for nerve regeneration applications. Materials Science and Engineering C, 63, 616–627. https://doi.org/10.1016/j.msec.2016.03.008
Wang, C., Wang, J., Zeng, L., Qiao, Z., Liu, X., Liu, H., … Ding, J. (2019). Fabrication of electrospun polymer nanofibers with diverse morphologies. Molecules, 24(5), 834. https://doi.org/10.3390/molecules24050834
Wang, K., Liu, L., Xie, J., Shen, L., Tao, J., & Zhu, J. (2018). Facile strategy to generate aligned polymer nanofibers: Effects on cell adhesion. ACS Applied Materials and Interfaces, 10(2), 1566–1574. https://doi.org/10.1021/acsami.7b16057
Wang, Y., Cui, W., Zhao, X., Wen, S., Sun, Y., Han, J., & Zhang, H. (2019). Bone remodeling-inspired dual delivery electrospun nanofibers for promoting bone regeneration. Nanoscale, 11(1), 60–71. https://doi.org/10.1039/c8nr07329e
Wang, Y., Jiang, Y., Zhang, Y., Wen, S., Wang, Y., & Zhang, H. (2019). Dual functional electrospun core-shell nanofibers for anti-infective guided bone regeneration membranes. Materials Science and Engineering C, 98, 134–139. https://doi.org/10.1016/j.msec.2018.12.115
Wendorff, J., Agarwal, S., & Greiner, A. (2012). Electrospinning: Materials, processing, and applications. Weinheim, Germany: Wiley-VCH Verlag GmbH & Co. KGaA.
Weng, L., Boda, S. K., Wang, H., Teusink, M. J., Shuler, F. D., & Xie, J. (2018). Novel 3D hybrid nanofiber aerogels coupled with BMP-2 peptides for cranial bone regeneration. Advanced Healthcare Materials, 7(10), e1701415. https://doi.org/10.1002/adhm.201701415
Wu, J., Wang, N., Zhao, Y., & Jiang, L. (2013). Electrospinning of multilevel structured functional micro-/nanofibers and their applications. Journal of Materials Chemistry A, 1(25), 7290–7305. https://doi.org/10.1039/c3ta10451f
Wu, J., & Hong, Y. (2016). Enhancing cell infiltration of electrospun fibrous scaffolds in tissue regeneration. Bioactive Materials, 1, 56–64. https://doi.org/10.1016/j.bioactmat.2016.07.001
Xie, J., Liu, W., Macewan, M. R., Bridgman, P. C., & Xia, Y. (2014). Neurite outgrowth on electrospun nanofibers with uniaxial alignment: The effects of fiber density, surface coating, and supporting substrate. ACS Nano, 8(2), 1878–1885. https://doi.org/10.1021/nn406363j
Xie, J., MacEwan, M. R., Li, X., Sakiyama-Elbert, S. E., & Xia, Y. (2009). Neurite outgrowth on nanofiber scaffolds with different orders, structures, and surface properties. ACS Nano, 3(5), 1151–1159. https://doi.org/10.1021/nn900070z
Xie, J., MacEwan, M. R., Schwartz, A. G., & Xia, Y. (2010). Electrospun nanofibers for neural tissue engineering. Nanoscale, 2(1), 35–44. https://doi.org/10.1039/b9nr00243j
Xu, S. C., Qin, C. C., Yu, M., Dong, R. H., Yan, X., Zhao, H., … Long, Y. Z. (2015). A battery-operated portable handheld electrospinning apparatus. Nanoscale, 7(29), 12351–12355. https://doi.org/10.1039/c5nr02922h
Xue, J., Wu, T., Dai, Y., & Xia, Y. (2019). Electrospinning and electrospun nanofibers: Methods, materials, and applications. Chemical Reviews, 119, 5298–5415. https://doi.org/10.1021/acs.chemrev.8b00593
Xue, J., Yang, J., O’Connor, D. M., Zhu, C., Huo, D., Boulis, N. M., & Xia, Y. (2017). Differentiation of bone marrow stem cells into Schwann cells for the promotion of neurite outgrowth on electrospun fibers. ACS Applied Materials and Interfaces, 9(14), 12299–12310. https://doi.org/10.1021/acsami.7b00882
Yamaguchi, K., Prabakaran, M., Ke, M., Gang, X., Chung, I. M., Um, I. C., … Kim, I. S. (2016). Highly dispersed nanoscale hydroxyapatite on cellulose nanofibers for bone regeneration. Materials Letters, 168, 56–61. https://doi.org/10.1016/j.matlet.2016.01.010
Yang, F., Murugan, R., Wang, S., & Ramakrishna, S. (2005). Electrospinning of nano/micro scale poly(l-lactic acid) aligned fibers and their potential in neural tissue engineering. Biomaterials, 26(15), 2603–2610. https://doi.org/10.1016/j.biomaterials.2004.06.051
Yang, G., Rothrauff, B. B., & Tuan, R. S. (2013). Tendon and ligament regeneration and repair: Clinical relevance and developmental paradigm. Birth Defects Research Part C—Embryo Today: Reviews, 99, 203–222. https://doi.org/10.1002/bdrc.21041
Yang, Y., Xia, T., Zhi, W., Wei, L., Weng, J., Zhang, C., & Li, X. (2011). Promotion of skin regeneration in diabetic rats by electrospun core-sheath fibers loaded with basic fibroblast growth factor. Biomaterials, 32(18), 4243–4254. https://doi.org/10.1016/j.biomaterials.2011.02.042
Yannas, I. V., Tzeranis, D. S., & So, P. T. C. (2017a). Regeneration mechanism for skin and peripheral nerves clarified at the organ and molecular scales. Current Opinion in Biomedical Engineering, 6, 1–7. https://doi.org/10.1016/j.cobme.2017.12.002
Yannas, I. V., Tzeranis, D. S., & So, P. T. C. (2017b). Regeneration of injured skin and peripheral nerves requires control of wound contraction, not scar formation. Wound Repair and Regeneration, 25(2), 177–191. https://doi.org/10.1111/wrr.12516
Zamborsky, R., Kilian, M., Csobonyeiova, M., & Danisovic, L. (2018). Regenerative medicine in orthopaedics and trauma: Challenges, regulation and ethical issues. Ortopedia Traumatologia Rehabilitacja, 20(3), 173–180. https://doi.org/10.5604/01.3001.0012.0764
Zhang, L., Chen, S., Liang, R., Chen, Y., Li, S., Li, S., … Yang, Y. (2018). Fabrication of alignment polycaprolactone scaffolds by combining use of electrospinning and micromolding for regulating Schwann cells behavior. Journal of Biomedical Materials Research—Part A, 106(12), 3123–3134. https://doi.org/10.1002/jbm.a.36507
Zhou, Y., Yang, D., Chen, X., Xu, Q., Lu, F., & Nie, J. (2008). Electrospun water-soluble carboxyethyl chitosan/poly(vinyl alcohol) nanofibrous membrane as potential wound dressing for skin regeneration. Biomacromolecules, 9(1), 349–354. https://doi.org/10.1021/bm7009015
Zhu, W., Masood, F., O’Brien, J., & Zhang, L. G. (2015). Highly aligned nanocomposite scaffolds by electrospinning and electrospraying for neural tissue regeneration. Nanomedicine: Nanotechnology, Biology, and Medicine, 11(3), 693–704. https://doi.org/10.1016/j.nano.2014.12.001
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Nature Switzerland AG
About this chapter
Cite this chapter
Balusamy, B., Senthamizhan, A., Uyar, T. (2019). Design and Development of Electrospun Nanofibers in Regenerative Medicine. In: Tekinay, A. (eds) Nanomaterials for Regenerative Medicine. Stem Cell Biology and Regenerative Medicine. Humana, Cham. https://doi.org/10.1007/978-3-030-31202-2_2
Download citation
DOI: https://doi.org/10.1007/978-3-030-31202-2_2
Published:
Publisher Name: Humana, Cham
Print ISBN: 978-3-030-31201-5
Online ISBN: 978-3-030-31202-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)