Abstract
In the previous chapter, we introduced a semi-quantitative miniaturized “FusBLU” assay. We herein described a new strategy for non-invasive TMPRSS2-ERG detection in patient urinary samples by coupling the isothermal reverse transcription-recombinase polymerization amplification (RT-RPA) to amplify TMPRSS2-ERG transcripts and directly detect the amplicons using surface-enhanced Raman scattering (SERS).
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References
Prensner JR, Rubin MA, Wei JT, Chinnaiyan AM (2012) Beyond PSA: the next generation of prostate cancer biomarkers. Sci Transl Med 4:127rv3
Kumar-Sinha C, Tomlins SA, Chinnaiyan AM (2008) Recurrent gene fusions in prostate cancer. Nat Rev Cancer 8:497–511
Tomlins SA et al (2005) Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer. Science 310:644–648
Clark J et al (2007) Diversity of TMPRSS2-ERG fusion transcripts in the human prostate. Oncogene 26:2667–2673
Clark JP, Cooper CS (2009) ETS gene fusions in prostate cancer. Nat Rev Urol 6:429–439
Koo KM, Wee EJH, Trau M (2016) Colorimetric TMPRSS2-ERG gene fusion detection in prostate cancer urinary samples via recombinase polymerase amplification. Theranostics 6:1415–1424
Yoshimoto M et al (2008) Absence of TMPRSS2:ERG fusions and PTEN losses in prostate cancer is associated with a favorable outcome. Mod Pathol 21:1451–1460
Koo KM, Wee EJH, Wang Y, Trau M (2017) Enabling miniaturised personalised diagnostics: from lab-on-a-chip to lab-in-a-drop. Lab Chip 17:3200–3220
Wang J, Koo KM, Wee EJH, Wang YL, Trau M (2017) A nanoplasmonic label-free surface-enhanced Raman scattering strategy for non-invasive cancer genetic subtyping in patient samples. Nanoscale 9:3496–3503
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Koo, K.M. (2019). Label-Free Surface-Enhanced Raman Scattering Detection System for Clinical Biomarker Targets. In: Advancing Gene Fusion Detection Towards Personalized Cancer Nanodiagnostics . Springer Theses. Springer, Cham. https://doi.org/10.1007/978-3-030-31000-4_3
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DOI: https://doi.org/10.1007/978-3-030-31000-4_3
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