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Polish Conference on Biocybernetics and Biomedical Engineering

PCBEE 2019: Current Trends in Biomedical Engineering and Bioimages Analysis pp 47–61Cite as

Role of Let-7 Family miRNAs in Migration of Colorectal Cancer HCT 116 and Caco-2 Cells After Stimulation by the Adipokine Vaspin. Time-Lapse Live-Cell Microscopic Observations

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Part of the book series: Advances in Intelligent Systems and Computing ((AISC,volume 1033))

Abstract

Changes of transcript levels are among the first observable cellular responses to internal or external stimuli. Those changes may be caused by the well known phenomena of RNA interference, especially by directly acting, transcript-specific micro RNAs (miRNAs), capable of influencing gene expression by activation or silencing at the level of DNA or mRNA. It may therefore be crucial to estimate the expression levels of both a mRNA and of the miRNAs influencing it. Micro RNA level changes may also be relevant in changes of the influence of signalling pathways on specific genes. One of the greatest threats to developed societies nowadays is obesity, which is connected to various health problems including colon or breast cancer [9, 34]. In many studies a connection between adipose tissue-derived hormonal activation and obesity can be observed. Adipokines are various regulators (growth factors, cytokines) of different signalling pathways, and could play a significant role in stimulation cell proliferation, cell cycle, angiogenesis, and also tumour growth and metastasis [21]. The let-7 miRNA family seems to be a good candidate to study adipose tissue-derived hormonal activation especially cancer migration and metastasis in cancer cells due to its involvement in conservative pathways with various nuclear factors like factor-B, Lin28/Let7 and TNF-alpha [15]. In this paper we have studied the regulatory role of miRNA let-7 in migration of HCT 116 colorectal cancer cells, and additionally in Caco-2 cells after hormonal stimulation with the adipokine vaspin. We found stimulatory effects of vaspin on Caco-2 cells at a dose of 3 ng/ml after 72 h of observations by time lapse live-cell microscopy in Wound Healing assays.

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Acknowledgements

This work was supported by grant No. 02/010/BK_18/0102 (Malgorzata Adamiec) from Silesian University of Technology in Gliwice, Poland; and grant No. UMO-2015/19/B/ST7/02984 (Dorota Hudy and Patryk Bil) founded by the Polish National Science Center. Magdalena Skonieczna received financial support (grant No. 02/010/RGH17/0092) funded by the Rector of the Silesian University of Technology in Gliwice, Poland. Dr. Eng. Sebastian Student and MSc. Eng. Tomasz Hejmo are acknowledged for help at microscopic Wound Healing assays.

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Authors and Affiliations

  1. System Engineering Group, Silesia University of Technology, Institute of Automatic Control, Akademicka 16, 44-100, Gliwice, Poland

    Magdalena Skonieczna, Dorota Hudy, Patryk Bil & Malgorzata Adamiec

  2. Biotechnology Center, Silesian University of Technology, Krzywoustego 8, 44-100, Gliwice, Poland

    Magdalena Skonieczna, Dorota Hudy, Patryk Bil & Malgorzata Adamiec

  3. District Hospital in Myszkow, Department of Internal Diseases, Aleja Wolności 29, 42-300, Myszków, Poland

    Marta Stachowska

  4. Department of Medical and Molecular Biology, Medical University of Silesia, School of Medicine with the Division of Dentistry, Jordana 19, 41-808, Zabrze, Poland

    Krzysztof Biernacki

Authors
  1. Magdalena Skonieczna
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  2. Dorota Hudy
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Correspondence to Magdalena Skonieczna .

Editor information

Editors and Affiliations

  1. Institute of Control and Computation Engineering, University of Zielona Gora, Zielona Góra, Poland

    Józef Korbicz

  2. Committee of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw, Poland

    Roman Maniewski

  3. Institute of Control and Computation Engineering, University of Zielona Góra, Zielona Góra, Poland

    Krzysztof Patan

  4. Institute of Control and Computation Engineering, University of Zielona Góra, Zielona Góra, Poland

    Marek Kowal

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Declaration of Conflicting Interests

The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Author Contributions

Magdalena Skonieczna conceived, designed and carried out the experiments and wrote the paper. Dorota Hudy analyzed the data; Patryk Bil analyzed time-lapse images; Malgorzata Adamiec prepared Figures and Wound Healing assays; Krzysztof Biernacki help at experiments for gene reporter assays. Marta Stachowska was involved in revising the paper’s important content. All authors read and approved the final manuscript.

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Skonieczna, M., Hudy, D., Bil, P., Adamiec, M., Stachowska, M., Biernacki, K. (2020). Role of Let-7 Family miRNAs in Migration of Colorectal Cancer HCT 116 and Caco-2 Cells After Stimulation by the Adipokine Vaspin. Time-Lapse Live-Cell Microscopic Observations. In: Korbicz, J., Maniewski, R., Patan, K., Kowal, M. (eds) Current Trends in Biomedical Engineering and Bioimages Analysis. PCBEE 2019. Advances in Intelligent Systems and Computing, vol 1033. Springer, Cham. https://doi.org/10.1007/978-3-030-29885-2_5

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  • DOI: https://doi.org/10.1007/978-3-030-29885-2_5

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