Abstract
Genetic transformation methods are a prerequisite for the functional analysis and modification of genes. About 30 years ago, first reports on the genetic transformation of filamentous fungi have been published, a breakthrough technology not only for scientific purposes but also for fungal biotechnology. Key transformation tools and approaches now routinely established in many labs working with filamentous fungi include PEG-mediated transformation of protoplasts, electroporation, biolistic transformation and Agrobacterium-mediated DNA transfer. However, each fungal species may require optimisation of existing protocols. This article aims to give an overview of transformation methods existing for genome manipulation in selected filamentous fungal model species. Pros and cons of each method will be discussed, respective examples will be given, and new developments in genome editing will be highlighted.
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Acknowledgements
We would like to thank our former BSc/MSc students Markus Delitz and Linda Salzmann for providing valuable background data on electroporation for this manuscript. The Tyrolean Science Fund (Tiroler Wissenschaftförderung, TWF; grant TWF-256524 to AL) and the Austrian Science Fund (FWF; grant P28248-B22 to SZ) are acknowledged for funding.
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Lichius, A., Ruiz, D.M., Zeilinger, S. (2020). Genetic Transformation of Filamentous Fungi: Achievements and Challenges. In: Nevalainen, H. (eds) Grand Challenges in Fungal Biotechnology. Grand Challenges in Biology and Biotechnology. Springer, Cham. https://doi.org/10.1007/978-3-030-29541-7_5
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