Abstract
Standard chemicals used for buffer preparation and crystallization were purchased from the following vendors, unless stated otherwise: Anatrace (USA), AppliChem (Germany), Avanti Polar Lipids (USA), Fluka (Germany), Glycon (Germany), Merck (Germany), Sigma-Aldrich (Germany), Serva (Germany), Roth (Germany), VWR (Germany).
Parts of this chapter have been reproduced from Bastian Bräuning, Eva Bertosin, Florian Praetorius, Christian Ihling, Alexandra Schatt, Agnes Adler, Klaus Richter, Andrea Sinz, Hendrik Dietz, Michael Groll: Structure & mechanism of the two-component α-helical pore-forming toxin YaxAB, Nature Communications 9 (1), 1806 (2018); https://doi.org/10.1038/s41467-018-04139-2.
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Bräuning, B. (2019). Materials and Methods. In: Structural and Biochemical Characterization of the YaxAB Pore-forming Toxin from Yersinia Enterocolitica. Springer Theses. Springer, Cham. https://doi.org/10.1007/978-3-030-29439-7_3
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