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Metabolic Profiling of the Mesenchymal Stem Cells’ Secretome

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Genomics, Proteomics, and Metabolomics

Part of the book series: Stem Cell Biology and Regenerative Medicine ((STEMCELL))

Abstract

Human ‘multipotent mesenchymal stromal cells’ (hMSCs) are popular cells in human regenerative medicine due to their ability to renew themselves and differentiate into various specialized cell types under specific physiological or experimental conditions. HMSCs secrete a broad spectrum of components including proteins and metabolites that represent significant effects on the cells in their neighborhood. Furthermore, it assists to characterize them. The therapeutic effects of hMSCs were thought to be due to their multipotent characterization and their ability to engraft and differentiate at the site of injury. However, recent studies have revealed the fact that a secretome plays important role in the therapeutic potential of hMSCs. Here, we outline the decoding of the metabolome for hMSCs secretome through metabolic profiling by application of MALDI-TOF-MS which could contribute to a better understanding of the therapeutic effects associated with hMSCs and their characterization.

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Author information

Authors and Affiliations

  1. Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium

    Kambiz Gilany

  2. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

    Kambiz Gilany

  3. Chemometrics Laboratory, Department of Chemistry, Tarbiat Modares University, Tehran, Iran

    Mohammad Javad Masroor & Ahmad Mani-Varnosfaderani

  4. Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

    Arash Minai-Tehrani

  5. Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Babak Arjmand

  6. Metabolomics and Genomics Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Babak Arjmand

Authors
  1. Kambiz Gilany
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  2. Mohammad Javad Masroor
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  3. Arash Minai-Tehrani
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  4. Ahmad Mani-Varnosfaderani
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  5. Babak Arjmand
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Corresponding author

Correspondence to Babak Arjmand .

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Editors and Affiliations

  1. Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism, Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Metabolomics and Genomics Research Center, Endocrinology and Metabolism, Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Babak Arjmand

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Gilany, K., Masroor, M.J., Minai-Tehrani, A., Mani-Varnosfaderani, A., Arjmand, B. (2019). Metabolic Profiling of the Mesenchymal Stem Cells’ Secretome. In: Arjmand, B. (eds) Genomics, Proteomics, and Metabolomics. Stem Cell Biology and Regenerative Medicine. Humana, Cham. https://doi.org/10.1007/978-3-030-27727-7_3

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