Abstract
Polymer-based eye drops are the most drug delivery system used to treat the dry eye disease (DED). Therefore, the mucoadhesion between the polymer and the ocular mucin is crucial to ensure the efficacy of the treatment. In this context, the present study aimed to evaluate the potential use of in vitro methods to study the mucoadhesion of two Hyaluronic Acid (HA)-based eye drops solutions, HA 0.15% and 0.3% (w/v) to treat DED. Two rheological methods were performed to study the mucoadhesive properties of both formulations: tackiness testing and oscillation frequency sweep. In vitro tests on ARPE-19 cell line were performed using a 2D and a 3D dry eye model. The results showed HA 0.3% appeared to be tackier than HA 0.15% in both cases. In vitro tests on ARPE-19 have shown that pre-treated cells with HA showed a morphology more similar to the hydrated cells in both products, with a high survival rate. The in vitro techniques used in this study have shown to be suitable to evaluate and predict mucoadhesive properties and the efficacy of the eye drops on relief or treat the DED. The results obtained from these methods may help in inferring possible in vivo effects.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Cwiklik, L.: Tear film lipid layer: a molecular level view. BBA Biomembranes 1858(10), 2421–2430 (2016). https://doi.org/10.1016/j.bbamem.2016.02.020
Ludwig, A.: The use of mucoadhesive polymers in ocular drug delivery. Adv. Drug Deliv. Rev. 57(11), 1595–1639 (2005). https://doi.org/10.1016/j.addr.2005.07.005
Graça, A., Gonçalves, L., Raposo, S., Ribeiro, H.M., Marto, J.: Useful in vitro techniques to evaluate the mucoadhesive properties of hyaluronic acid-based ocular delivery systems. Pharmaceutics 10, 110 (2018). https://doi.org/10.3390/pharmaceutics10030110
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Nature Switzerland AG
About this paper
Cite this paper
Graça, A., Gonçalves, L.M., Raposo, S., Ribeiro, H.M., Marto, J. (2020). Ocular Lubricants Efficacy: Mucoadhesive Evaluation Using Rheological Methods. In: Galindo-Rosales, F., Campo-Deaño, L., Afonso, A., Alves, M., Pinho, F. (eds) Proceedings of the Iberian Meeting on Rheology (IBEREO 2019). IBEREO 2019. Springer Proceedings in Materials. Springer, Cham. https://doi.org/10.1007/978-3-030-27701-7_7
Download citation
DOI: https://doi.org/10.1007/978-3-030-27701-7_7
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-27700-0
Online ISBN: 978-3-030-27701-7
eBook Packages: EngineeringEngineering (R0)