Abstract
Deregulation of microRNA expression has been shown to play an important role in human malignancies. The identification of circulating-free miRNAs in biofluids a decade ago led to great enthusiasm and motivation to develop non-invasive tests based on the expression of these small non-coding RNAs. Herein, we review the progress within the field of research for identifying circulating miRNA cancer biomarkers and discuss the advantages and challenges associated with this. We also discuss the methodological and analytical variables, which may influence the final miRNA quantification and the importance of standardizing pre-analytical, analytical, and post-analytical processes in order to enable a successful translation of the results from basic research into the clinics.
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Abbreviations
- EDTA:
-
Ethylenediaminetetraacetic acid
- HDL:
-
High-density lipoproteins
- LDCT:
-
Low-dose computed tomography
- MGCT:
-
Malignant germ cell tumors
- miRNA:
-
MicroRNA
- MSC:
-
miRNA signature classifier
- Nt:
-
Nucleotides
- qPCR:
-
Quantitative polymerase chain reaction
- snoRNA:
-
Small nucleolar RNA
- UTR:
-
Untranslated region
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Andersen, G.B., Tost, J. (2020). Circulating miRNAs as Biomarker in Cancer. In: Schaffner, F., Merlin, JL., von Bubnoff, N. (eds) Tumor Liquid Biopsies. Recent Results in Cancer Research, vol 215. Springer, Cham. https://doi.org/10.1007/978-3-030-26439-0_15
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