Abstract
Microalgae metabolism is powered only by sustainable energy and carbon sources, representing a valuable alternative to develop clean industrial processes. Moreover, this group of unicellular photosynthetic microorganisms shows high versatility, including species from different ecological niches which evolved a variety of pathways to synthesize a wide spectrum of bioactive compounds. However, sophisticated industrial cultivation systems are needed to control the stability of the production process during intensive cultivation. This artificial environment is far different from the ecological niches that shaped these organisms, limiting photon-to-biomass conversion efficiency (PBCE) to values far below those achieved at the lab scale. Moreover, large-scale cultivation has high energetic and operational costs due to initial investment and maintenance, that current PBCE values cannot compensate for, preventing commercial feasibility. Tuning microalgae photosynthetic metabolism represents an unavoidable challenge to improve PBCE and meet the theoretical potential of these organisms.
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Acién Fernández FG, Alı́as CB, Garcı́a-Malea López MC, Fernández Sevilla JM, Ibáñez González MJ, Gómez RN, Molina Grima E (2003) Assessment of the production of 13C labeled compounds from phototrophic microalgae at laboratory scale. Biomol Eng 20:149–162
Alboresi A, Storti M, Morosinotto T (2018) Balancing protection and efficiency in the regulation of photosynthetic electron transport across plant evolution. New Phytol 1859:676–683
Badger MR, Bek EJ (2008) Multiple Rubisco forms in proteobacteria: their functional significance in relation to CO2 acquisition by the CBB cycle. J Exp Bot 59:1525–1541
Bamba BSB, Lozano P, Adjé F, Ouattara A, Vian MA, Tranchant C, Lozano Y (2015) Effects of temperature and other operational parameters on Chlorella vulgaris mass cultivation in a simple and low-cost column photobioreactor. Appl Biochem Biotechnol 177:389–406
Banerjee A, Banerjee C, Negi S, Chang J-S, Shukla P (2018) Improvements in algal lipid production: a systems biology and gene editing approach. Crit Rev Biotechnol 38:369–385
Barber J, Andersson B (1992) Too much of a good thing: light can be bad for photosynthesis. Trends Biochem Sci 17:61–66
Bar-Even A (2018) Daring metabolic designs for enhanced plant carbon fixation. Plant Sci 273:71–83
Bar-Even A, Noor E, Savir Y, Liebermeister W, Davidi D, Tawfik DS, Milo R (2011) The moderately efficient enzyme: evolutionary and physicochemical trends shaping enzyme parameters. Biochemistry 50:19
Béchet Q, Chambonnière P, Shilton A, Guizard G, Guieysse B (2015) Algal productivity modeling: a step toward accurate assessments of full-scale algal cultivation. Biotechnol Bioeng 112:987–996
Beckmann J, Lehr F, Finazzi G, Hankamer B, Posten C, Wobbe L, Kruse O (2009) Improvement of light to biomass conversion by de-regulation of light-harvesting protein translation in Chlamydomonas reinhardtii. J Biotechnol 142:70–77
Bernardi A, Perin G, Sforza E, Galvanin F, Morosinotto T, Bezzo F (2014) An identifiable state model to describe light intensity influence on microalgae growth. Ind Eng Chem Res 53:6738–6749
Bernardi A, Meneghesso A, Morosinotto T, Bezzo F (2016) A model-based investigation of genetically modified microalgae strains. In: Computer aided chemical engineering
Berteotti S, Ballottari M, Bassi R (2016) Increased biomass productivity in green algae by tuning non-photochemical quenching. Sci Rep 6:21339
Bilal M, Rasheed T, Ahmed I, Iqbal HMN, Sada EG (2017) High-value compounds from microalgae with industrial exploitability – a review. Front Biosci 9:319–342
Blankenship RE, Tiede DM, Barber J, Brudvig GW, Fleming G, Ghirardi M, Gunner MR, Junge W, Kramer DM, Melis A, Moore TA, Moser CC, Nocera DG, Nozik AJ, Ort DR, Parson WW, Prince RC, Sayre RT (2011) Comparing photosynthetic and photovoltaic efficiencies and recognizing the potential for improvement. Science 332:805–809
Bonente G, Formighieri C, Mantelli M, Catalanotti C, Giuliano G, Morosinotto T, Bassi R (2011) Mutagenesis and phenotypic selection as a strategy toward domestication of Chlamydomonas reinhardtii strains for improved performance in photobioreactors. Photosynth Res 108:107–120
Brennan L, Owende P (2010) Biofuels from microalgae—a review of technologies for production, processing, and extractions of biofuels and co-products. Renew Sust Energ Rev 14:557–577
Büchel C (2015) Evolution and function of light harvesting proteins. J Plant Physiol 172:62–75
Bule MH, Ahmed I, Maqbool F, Bilal M, Iqbal HMN (2018) Microalgae as a source of high-value bioactive compounds. Front Biosci (Schol Ed) 10:197–216
Cardol P, Forti G, Finazzi G (2011) Regulation of electron transport in microalgae. Biochim Biophys Acta 1807:912–918
Cardona T, Shao S, Nixon PJ (2018) Enhancing photosynthesis in plants: the light reactions. Essays Biochem 62:85–94
Carmo-Silva E, Scales JC, Madgwick PJ, Parry MAJ (2015) Optimizing Rubisco and its regulation for greater resource use efficiency. Plant Cell Environ 38:1817–1832
Carneiro MLNM, Pradelle F, Braga SL, Gomes MSP, Martins ARFA, Turkovics F, Pradelle RNC (2017) Potential of biofuels from algae: comparison with fossil fuels, ethanol and biodiesel in Europe and Brazil through life cycle assessment (LCA). Renew Sust Energ Rev 73:632–653
Carvalho AP, Silva SO, Baptista JM, Malcata FX (2011) Light requirements in microalgal photobioreactors: an overview of biophotonic aspects. Appl Microbiol Biotechnol 89:1275–1288
Cazzaniga S, Dall’Osto L, Szaub J, Scibilia L, Ballottari M, Purton S, Bassi R (2014) Domestication of the green alga Chlorella sorokiniana: reduction of antenna size improves light-use efficiency in a photobioreactor. Biotechnol Biofuels 7:157
Cheah WY, Show PL, Chang J-S, Ling TC, Juan JC (2015) Biosequestration of atmospheric CO2 and flue gas-containing CO2 by microalgae. Bioresour Technol 184:190–201
Chen M, Blankenship RE (2011) Expanding the solar spectrum used by photosynthesis. Trends Plant Sci 16:427–431
Cotton CAR, Douglass JS, De Causmaecker S, Brinkert K, Cardona T, Fantuzzi A, Rutherford AW, Murray JW (2015) Photosynthetic constraints on fuel from microbes. Front Bioeng Biotechnol 3:36
Cotton CA, Edlich-Muth C, Bar-Even A (2018) Reinforcing carbon fixation: CO2 reduction replacing and supporting carboxylation. Curr Opin Biotechnol 49:49–56
Dalal J, Lopez H, Vasani NB, Hu Z, Swift JE, Yalamanchili R, Dvora M, Lin X, Xie D, Qu R, Sederoff HW (2015) A photorespiratory bypass increases plant growth and seed yield in biofuel crop Camelina sativa. Biotechnol Biofuels 8:175
De Mooij T, Janssen M, Cerezo-Chinarro O, Mussgnug JH, Kruse O, Ballottari M, Bassi R, Bujaldon S, Wollman F-A, Wijffels RH (2014) Antenna size reduction as a strategy to increase biomass productivity: a great potential not yet realized. J Appl Phycol 27:1063–1077
De Porcellinis AJ, Nørgaard H, Brey LMF, Erstad SM, Jones PR, Heazlewood JL, Sakuragi Y (2018) Overexpression of bifunctional fructose-1,6-bisphosphatase/sedoheptulose-1,7-bisphosphatase leads to enhanced photosynthesis and global reprogramming of carbon metabolism in Synechococcus sp. PCC 7002. Metab Eng 47:170–183
De Vree JH, Bosma R, Janssen M, Barbosa MJ, Wijffels RH (2015) Comparison of four outdoor pilot-scale photobioreactors. Biotechnol Biofuels 8:1–12
Dejtisakdi W, Miller SM (2016) Overexpression of Calvin cycle enzyme fructose 1,6-bisphosphatase in Chlamydomonas reinhardtii has a detrimental effect on growth. Algal Res 14:116–126
Ding F, Wang M, Zhang S, Ai X (2016) Changes in SBPase activity influence photosynthetic capacity, growth, and tolerance to chilling stress in transgenic tomato plants. Sci Rep 6:32741
Durall C, Lindblad P (2015) Mechanisms of carbon fixation and engineering for increased carbon fixation in cyanobacteria. Algal Res 11:263–270
Eberhard S, Finazzi G, Wollman F-A (2008) The dynamics of photosynthesis. Annu Rev Genet 42:463–515
Eilers P, Peeters J (1988) A Model for the reletionship between light-intensity and the rate of photosynthesis in phytoplankton. Ecol Model 42:199–215
Erb TJ, Jones PR, Bar-Even A (2017) Synthetic metabolism: metabolic engineering meets enzyme design. Curr Opin Chem Biol 37:56–62
Fang L, Lin HX, Low CS, Wu MH, Chow Y, Lee YK (2012) Expression of the Chlamydomonas reinhardtii sedoheptulose-1,7-bisphosphatase in Dunaliella bardawil leads to enhanced photosynthesis and increased glycerol production. Plant Biotechnol J 10:1129–1135
Fargione J, Hill J, Tilman D, Polasky S, Hawthorne P (2008) Land clearing and the biofuel carbon debt. Science 319:1235–1238
Feng L, Li H, Jiao J, Li D, Zhou L, Wan J, Li Y (2009) Reduction in SBPase activity by antisense RNA in transgenic rice plants: effect on photosynthesis, growth, and biomass allocation at different nitrogen levels. J Plant Biol 52:382–394
Ferenczi A, Euan D, Xipnitou A, Molnar A (2017) Efficient targeted DNA editing and replacement in Chlamydomonas reinhardtii using Cpf1 ribonucleoproteins and single-stranded DNA. PNAS 114:13567–13572
Flügel F, Timm S, Arrivault S, Florian A, Stitt M, Fernie AR, Bauwe H (2017) The photorespiratory metabolite 2-phosphoglycolate regulates photosynthesis and starch accumulation in arabidopsis. Plant Cell 29:2537–2551
Formighieri C, Franck F, Bassi R (2012) Regulation of the pigment optical density of an algal cell: filling the gap between photosynthetic productivity in the laboratory and in mass culture. J Biotechnol 162:115–123
Gee CW, Niyogi KK (2017) The carbonic anhydrase CAH1 is an essential component of the carbon-concentrating mechanism in Nannochloropsis oceanica. Proc Natl Acad Sci 114:4537–4542
Gerotto C, Alboresi A, Meneghesso A, Jokel M, Suorsa M, Aro E-MEM, Morosinotto T (2016) Flavodiiron proteins act as safety valve for electrons in Physcomitrella patens. Proc Natl Acad Sci USA 113:12322–12327
Gies E (2017) The real cost of energy. Nature 551:S145–S147
Giordano M, Beardall J, Raven JA (2005) CO2 concentrating mechanisms in algae: mechanisms, environmental modulation, and evolution. Annu Rev Plant Biol 56:99–131
Gómez R, Carrillo N, Morelli MP, Tula S (2018) Faster photosynthetic induction in tobacco by expressing cyanobacterial flavodiiron proteins in chloroplasts. Photosynth Res 136:129–138
Goss R, Jakob T (2010) Regulation and function of xanthophyll cycle-dependent photoprotection in algae. Photosynth Res 106:103–122
Goss R, Lepetit B (2015) Biodiversity of NPQ. J Plant Physiol 172C:13–32
Hagemann M, Bauwe H (2016) Photorespiration and the potential to improve photosynthesis. Curr Opin Chem Biol 35:109–116
Hauser T, Popilka L, Hartl FU, Hayer-Hartl M (2015) Role of auxiliary proteins in Rubisco biogenesis and function. Nat Plants 1:15065
Hayer-Hartl M (2017) From chaperonins to Rubisco assembly and metabolic repair. Protein Sci 26:2324–2333
Ho M-Y, Shen G, Canniffe DP, Zhao C, Bryant DA (2016) Light-dependent chlorophyll f synthase is a highly divergent paralog of PsbA of photosystem II. Science 353:aaf9178–aaf9178
Hudson GS, Evans JR, von Caemmerer S, Arvidsson YB, Andrews TJ (1992) Reduction of ribulose-1,5-bisphosphate carboxylase/oxygenase content by antisense RNA reduces photosynthesis in transgenic tobacco plants. Plant Physiol 98:294–302
Hwangbo K, Lim J-M, Jeong S-W, Vikramathithan J, Park Y-I, Jeong W-J (2018) Elevated inorganic carbon concentrating mechanism confers tolerance to high light in an arctic Chlorella sp. ArM0029B. Front Plant Sci 9:590
Ichikawa Y, Tamoi M, Sakuyama H, Maruta T, Ashida H, Yokota A, Shigeoka S (2010) Generation of transplastomic lettuce with enhanced growth and high yield. GM Crops 1:322–326
Iwaki T, Haranoh K, Inoue N, Kojima K, Satoh R, Nishino T, Wada S, Ihara H, Tsuyama S, Kobayashi H, Wadano A (2006) Expression of foreign type I ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) stimulates photosynthesis in cyanobacterium Synechococcus PCC7942 cells. Photosynth Res 88:287–297
Jeong J, Baek K, Yu J, Kirst H, Betterle N, Shin W (2018) Deletion of the chloroplast LTD protein impedes LHCI import and PSI – LHCI assembly in Chlamydomonas reinhardtii. J Exp Bot 69:1147–1158
Kato Y, Sun X, Zhang L, Sakamoto W (2012) Cooperative D1 degradation in the photosystem II repair mediated by chloroplastic proteases in Arabidopsis. Plant Physiol 159:1428–1439
Kebeish R, Niessen M, Thiruveedhi K, Bari R, Hirsch H-J, Rosenkranz R, Stäbler N, Schönfeld B, Kreuzaler F, Peterhänsel C (2007) Chloroplastic photorespiratory bypass increases photosynthesis and biomass production in Arabidopsis thaliana. Nat Biotechnol 25:593–599
Kim HS, Weiss TL, Thapa HR, Devarenne TP, Han A (2014) A microfluidic photobioreactor array demonstrating high-throughput screening for microalgal oil production. Lab Chip 14:1415–1425
Kirk J (1994) Light and photosynthesis in aquatic ecosystems, 2nd edn. Cambridge University Press, Cambridge
Kirst H, Melis A (2014) The chloroplast signal recognition particle (CpSRP) pathway as a tool to minimize chlorophyll antenna size and maximize photosynthetic productivity. Biotechnol Adv 32:66–72
Kirst H, García-Cerdán JG, Zurbriggen A, Melis A (2012a) Assembly of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii requires expression of the TLA2-CpFTSY gene. Plant Physiol 158:930–945
Kirst H, Garcia-Cerdan JG, Zurbriggen A, Ruehle T, Melis A (2012b) Truncated photosystem chlorophyll antenna size in the green microalga Chlamydomonas reinhardtii upon deletion of the TLA3-CpSRP43 gene. Plant Physiol 160:2251–2260
Kirst H, Formighieri C, Melis A (2014) Maximizing photosynthetic efficiency and culture productivity in cyanobacteria upon minimizing the phycobilisome light-harvesting antenna size. Biochim Biophys Acta 1837:1653–1664
Koutra E, Economou CN, Tsafrakidou P, Kornaros M (2018) Bio-based products from microalgae cultivated in digestates. Trends Biotechnol 36:819–833
Kromdijk J, Głowacka K, Leonelli L, Gabilly ST, Iwai M, Niyogi KK, Long SP (2016) Improving photosynthesis and crop productivity by accelerating recovery from photoprotection. Science 354:857–861
Kuczynska P, Jemiola-Rzeminska M, Strzalka K (2015) Photosynthetic pigments in diatoms. Mar Drugs 13:5847–5881
Lam GP, Vermuë MH, Eppink MHM, Wijffels RH, Van Den Berg C (2018) Multi-product microalgae biorefineries: from concept towards reality. Trends Biotechnol 36:216–227
Larosa V, Meneghesso A, La Rocca N, Steinbeck J, Hippler M, Szabò I, Morosinotto T (2018) Mitochondria affect photosynthetic electron transport and photosensitivity in a green alga. Plant Physiol 176:2305–2314
Levey M, Timm S, Mettler-Altmann T, Luca Borghi G, Koczor M, Arrivault S, Pm Weber A, Bauwe H, Gowik U, Westhoff P (2018) Efficient 2-phosphoglycolate degradation is required to maintain carbon assimilation and allocation in the C4 plant Flaveria bidentis. J Exp Bot 70:575–587
Liang F, Lindblad P (2016) Effects of overexpressing photosynthetic carbon flux control enzymes in the cyanobacterium Synechocystis PCC 6803. Metab Eng 38:56–64
Liang F, Lindblad P (2017) Synechocystis PCC 6803 overexpressing RuBisCO grow faster with increased photosynthesis. Metab Eng Commun 4:29–36
Luke CS, Selimkhanov J, Baumgart L, Cohen SE, Golden SS, Cookson NA, Hasty J (2016) A microfluidic platform for long-term monitoring of algae in a dynamic environment. ACS Synth Biol 5:8–14
Maeda Y, Yoshino T, Matsunaga T, Matsumoto M, Tanaka T (2018) ScienceDirect Marine microalgae for production of biofuels and chemicals. Curr Opin Biotechnol 50:111–120
Melis A (1991) Dynamics of photosynthetic membrane composition and function. BBA-Bioenergetics 1058:87–106
Melis A (2009) Solar energy conversion efficiencies in photosynthesis: minimizing the chlorophyll antennae to maximize efficiency. Plant Sci 177:272–280
Melis A, Neidhardt J, Benemann JR (1998) Dunaliella salina (Chlorophyta) with small chlorophyll antenna sizes exhibit higher photosynthetic productivities and photon use efficiencies than normally pigmented cells. J Appl Phycol 10:515–525
Meneghesso A, Simionato D, Gerotto C, La Rocca N, Finazzi G, Morosinotto T (2016) Photoacclimation of photosynthesis in the Eustigmatophycean Nannochloropsis gaditana. Photosynth Res 129:291–305
Molina E, Ferna J, Acie FG, Chisti Y (2001) Tubular photobioreactor design for algal cultures. J Biotechnol 92:113–131
Murchie EH, Niyogi KK (2011) Manipulation of photoprotection to improve plant photosynthesis. Plant Physiol 155:86–92
Nakajima Y, Itayama T (2003) Analysis of photosynthetic productivity of microalgal mass cultures. J Appl Phycol 15:497–505
Nakajima Y, Ueda R (1997) Improvement of photosynthesis in dense microalgal suspension by reduction of light harvesting pigments. J Appl Phycol 9:503–510
Nakajima Y, Ueda R (2000) The effect of reducing light-harvesting pigment on marine microalgal productivity. J Appl Phycol 12:285–290
Nakajima Y, Tsuzuki M, Ueda R (2001) Improved productivity by reduction of the content of light-harvesting pigment in Chlamydomonas perigranulata. J Appl Phycol 13:95–101
Ng I, Tan S, Kao P, Chang Y, Chang J (2017) Recent developments on genetic engineering of microalgae for biofuels and bio-based chemicals. Biotechnol J 1600644:1–13
Nikolaou A, Bernardi A, Meneghesso A, Bezzo F, Morosinotto T, Chachuat B (2015) A model of chlorophyll fluorescence in microalgae integrating photoproduction, photoinhibition and photoregulation. J Biotechnol 194:91–99
Nilkens M, Kress E, Lambrev P, Miloslavina Y, Müller M, Holzwarth AR, Jahns P (2010) Identification of a slowly inducible zeaxanthin-dependent component of non-photochemical quenching of chlorophyll fluorescence generated under steady-state conditions in Arabidopsis. Biochim Biophys Acta 1797:466–475
Nölke G, Houdelet M, Kreuzaler F, Peterhänsel C, Schillberg S (2014) The expression of a recombinant glycolate dehydrogenase polyprotein in potato (Solanum tuberosum) plastids strongly enhances photosynthesis and tuber yield. Plant Biotechnol J 12:734–742
Nölke G, Barsoum M, Houdelet M, Arcalís E, Kreuzaler F, Fischer R, Schillberg S (2018) The integration of algal carbon concentration mechanism components into tobacco chloroplasts increases photosynthetic efficiency and biomass. Biotechnol J 14:e1800170
Nymark M, Sharma AK, Sparstad T, Bones AM, Winge P (2016) A CRISPR/Cas9 system adapted for gene editing in marine algae. Sci Rep 6:24951
Ogawa T, Tamoi M, Kimura A, Mine A, Sakuyama H, Yoshida E, Maruta T, Suzuki K, Ishikawa T, Shigeoka S (2015) Enhancement of photosynthetic capacity in Euglena gracilis by expression of cyanobacterial fructose-1,6−/sedoheptulose-1,7-bisphosphatase leads to increases in biomass and wax ester production. Biotechnol Biofuels 8:80
Ooms MD, Dinh CT, Sargent EH, Sinton D (2016) Photon management for augmented photosynthesis. Nat Commun 7:12699
Ort DR, Melis A (2011) Optimizing antenna size to maximize photosynthetic efficiency. Plant Physiol 155:79–85
Ort DR, Merchant SS, Alric J, Barkan A, Blankenship RE, Bock R, Croce R, Hanson MR, Hibberd JM, Long SP, Moore TA, Moroney J, Niyogi KK, Parry MAJ, Peralta-Yahya PP, Prince RC, Redding KE, Spalding MH, van Wijk KJ, Vermaas WFJ, von Caemmerer S, Weber APM, Yeates TO, Yuan JS, Zhu XG (2015) Redesigning photosynthesis to sustainably meet global food and bioenergy demand. Proc Natl Acad Sci USA 112:8529–8536
Page LE, Liberton M, Pakrasi HB (2012) Reduction of photoautotrophic productivity in the cyanobacterium Synechocystis sp. strain PCC 6803 by phycobilisome antenna truncation. Appl Environ Microbiol 78:6349–6351
Park H, Lee C (2016) Theoretical calculations on the feasibility of microalgal biofuels: utilization of marine resources could help realizing the potential of microalgae. Biotechnol J 11:1461–1470
Parry MAJ, Madgwick PJ, Carvalho JFC, Andralojc PJ (2007) Prospects for increasing photosynthesis by overcoming the limitations of Rubisco. J Agric Sci 145:31
Perin G, Bellan A, Segalla A, Meneghesso A, Alboresi A, Morosinotto T (2015) Generation of random mutants to improve light-use efficiency of Nannochloropsis gaditana cultures for biofuel production. Biotechnol Biofuels 8:161
Perin G, Cimetta E, Monetti F, Morosinotto T, Bezzo F (2016) Novel micro-photobioreactor design and monitoring method for assessing microalgae response to light intensity. Algal Res 19:69–76
Perin G, Bernardi A, Bellan A, Bezzo F, Morosinotto T (2017a) A mathematical model to guide genetic engineering of photosynthetic metabolism. Metab Eng 44:337–347
Perin G, Simionato D, Bellan A, Carone M, Occhipinti A, Maffei ME, Morosinotto T (2017b) Cultivation in industrially relevant conditions has a strong influence on biological properties and performances of Nannochloropsis gaditana genetically modified strains. Algal Res 28:88–99
Perin G, Bellan A, Bernardi A, Bezzo F, Morosinotto T (2018) The potential of quantitative models to improve microalgae photosynthetic efficiency. Physiol Plant 166:380–391
Poliner E, Takeuchi T, Du ZY, Benning C, Farré EM (2018) Nontransgenic marker-free gene disruption by an episomal CRISPR system in the oleaginous microalga, Nannochloropsis oceanica CCMP1779. ACS Synth Biol 7:962–968
Polle JEW, Benemann JR, Tanaka A, Melis A (2000) Photosynthetic apparatus organization and function in the wild type and a chlorophyll b-less mutant of Chlamydomonas reinhardtii. Dependence on carbon source. Planta 211:335–344
Polle JEW, Kanakagiri S-DD, Melis A (2003) tla1, a DNA insertional transformant of the green alga Chlamydomonas reinhardtii with a truncated light-harvesting chlorophyll antenna size. Planta 217:49–59
Ramos Tercero EA, Sforza E, Morandini M, Bertucco A (2014) Cultivation of Chlorella protothecoides with urban wastewater in continuous photobioreactor: biomass productivity and nutrient removal. Appl Biochem Biotechnol 172:1470–1485
Renuka N, Guldhe A, Prasanna R, Singh P, Bux F (2018) Microalgae as multi-functional options in modern agriculture: current trends, prospects and challenges. Biotechnol Adv 36:1255–1273
Riebesell U, Wolf-Gladrow DA, Smetacek V (1993) Carbon dioxide limitation of marine phytoplankton growth rates. Nature 361:249–251
Robertson GP, Hamilton SK, Barham BL, Dale BE, Izaurralde RC, Jackson RD, Landis DA, Swinton SM, Thelen KD, Tiedje JM (2017) Cellulosic biofuel contributions to a sustainable energy future: choices and outcomes. Science 356:eaal2324
Rochaix J-D (2014) Regulation and dynamics of the light-harvesting system. Annu Rev Plant Biol 65:287–309
Rockström J, Gaffney O, Rogelj J, Meinshausen M, Nakicenovic N, Schellnhuber HJ (2017) A roadmap for rapid decarbonization. Science 355:1269–1271
Rosgaard L, de Porcellinis AJ, Jacobsen JH, Frigaard N-U, Sakuragi Y (2012) Bioengineering of carbon fixation, biofuels, and biochemicals in cyanobacteria and plants. J Biotechnol 162:134–147
Rubio FC, Camacho FG, Sevilla JMF, Chisti Y, Grima EM (2003) A mechanistic model of photosynthesis in microalgae. Biotechnol Bioeng 81:459–473
Ruffing AM (2014) Improved free fatty acid production in cyanobacteria with Synechococcus sp. PCC 7002 as host. Front Bioeng Biotechnol 2:17
Ruiz J, Olivieri G, de Vree J, Bosma R, Willems P, Reith JH, Eppink MHM, Kleinegris DMM, Wijffels RH, Barbosa MJ (2016) Towards industrial products from microalgae. Energy Environ Sci 9:3036–3043
Ruiz-Sola MÁ, Petroutsos D (2018) A toolkit for the characterization of the photoprotective capacity of green algae. Methods Mol Biol (Clifton, NJ) 1829:315–323
Rulli MC, Bellomi D, Cazzoli A, De Carolis G, Odorico PD (2016) The water-land-food nexus of first-generation biofuels. Sci Rep 6:22521
Salih FM (2011) Microalgae tolerance to high concentrations of carbon dioxide: a review. J Environ Prot (Irvine, Calif) 02:648–654
Saltelli A, Ratto M, Andres T, Campolongo F, Cariboni J, Gatelli D, Saisana M, Tarantola S (2007) Global sensitivity analysis. The primer, global sensitivity analysis. The primer. Wiley, Chichester
Santos FM, Pires JCM (2018) Nutrient recovery from wastewaters by microalgae and its potential application as bio-char. Bioresour Technol 267:725–731
Savir Y, Noor E, Milo R, Tlusty T (2010) Cross-species analysis traces adaptation of Rubisco toward optimality in a low-dimensional landscape. Proc Natl Acad Sci 107:3475–3480
Sharon-Gojman R, Leu S, Zarka A (2017) Antenna size reduction and altered division cycles in self-cloned, marker-free genetically modified strains of Haematococcus pluvialis. Algal Res 28:172–183
Shin W-S, Lee B, Jeong B, Chang YK, Kwon J-H (2016) Truncated light-harvesting chlorophyll antenna size in Chlorella vulgaris improves biomass productivity. J Appl Phycol 28:3193–3202
Simionato D, Basso S, Giacometti GMGM, Morosinotto T (2013) Optimization of light use efficiency for biofuel production in algae. Biophys Chem 182:71–78
Somerville CR (2001) An early Arabidopsis demonstration. Resolving a few issues concerning photorespiration. Plant Physiol 125:20–24
Tiwari A, Mamedov F, Grieco M, Suorsa M, Jajoo A, Styring S, Tikkanen M, Aro E-M (2016) Photodamage of iron–sulphur clusters in photosystem I induces non-photochemical energy dissipation. Nat Plants 2:16035
Tomar V, Sidhu GK, Nogia P, Mehrotra R, Mehrotra S (2017) Regulatory components of carbon concentrating mechanisms in aquatic unicellular photosynthetic organisms. Plant Cell Rep 36:1671–1688
Trudeau DL, Edlich-Muth C, Zarzycki J, Scheffen M, Goldsmith M, Khersonsky O, Avizemer Z, Fleishman SJ, Cotton CAR, Erb TJ, Tawfik DS, Bar-Even A (2018) Design and in vitro realization of carbon-conserving photorespiration. Proc Natl Acad Sci 115:E11455–E11464
Verruto J, Francis K, Wang Y, Low MC, Greiner J, Tacke S, Kuzminov F, Lambert W, McCarren J, Ajjawi I, Bauman N, Kalb R, Hannum G, Moellering ER (2018) Unrestrained markerless trait stacking in Nannochloropsis gaditana through combined genome editing and marker recycling technologies. Proc Natl Acad Sci USA 115:E7015–E7022
Walsh BJ, Rydzak F, Palazzo A, Kraxner F, Herrero M, Schenk PM, Ciais P, Janssens IA, Peñuelas J, Niederl-Schmidinger A, Obersteiner M (2015) New feed sources key to ambitious climate targets. Carbon Balance Manag 10:26
Wang Y, Stessman DJ, Spalding MH (2015) The CO2 concentrating mechanism and photosynthetic carbon assimilation in limiting CO2: how Chlamydomonas works against the gradient. Plant J 82:429–448
Wingler A, Lea PJ, Quick WP, Leegood RC (2000) Photorespiration: metabolic pathways and their role in stress protection. Philos Trans R Soc B Biol Sci 355:1517–1529
Wobbe L, Remacle C (2014) Improving the sunlight-to-biomass conversion efficiency in microalgal biofactories. J Biotechnol 201:28–42
Wobbe L, Blifernez O, Schwarz C, Mussgnug JH, Nickelsen J, Kruse O (2009) Cysteine modification of a specific repressor protein controls the translational status of nucleus-encoded LHCII mRNAs in Chlamydomonas. Proc Natl Acad Sci USA 106:13290–13295
Yamamoto H, Takahashi S, Badger MR, Shikanai T (2016) Artificial remodelling of alternative electron flow by flavodiiron proteins in Arabidopsis. Nat Plants 2:16012
Yun YS, Park JM (2001) Attenuation of monochromatic and polychromatic lights in Chlorella vulgaris suspensions. Appl Microbiol Biotechnol 55:765–770
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Perin, G., Morosinotto, T. (2019). Optimization of Microalgae Photosynthetic Metabolism to Close the Gap with Potential Productivity. In: Hallmann, A., Rampelotto, P. (eds) Grand Challenges in Algae Biotechnology. Grand Challenges in Biology and Biotechnology. Springer, Cham. https://doi.org/10.1007/978-3-030-25233-5_6
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