Abstract
Nanocarriers play an indispensible role in drug delivery mechanisms thereby increasing the efficiency and overall activity of the specified drug in the given dosage. Chitosan is a well known biocompatible molecule that is exploited for various in vivo applications. In this current study, a biocompatible nanocarrier is developed as a conjugate using chitosan nanoparticles for the drug Doxorubicin. A nanoparticle is synthesized using the green extract of a medicinal plant. Upon successful synthesis, a chitosan conjugate is prepared along with the drug and synthesized nanoparticles. The synthesis of gold nanomaterials using Catharanthus roseus (Nithyakalyani) is successfully carried out. The initial confirmation of the nanoconjugate is from UV visible spectrophotometric analysis, which showed the characteristic peak for nanoparticles. SEM & HRSEM analysis revealed the size and shape of the nanocojugate. The Zeta potential is measured to check the stability of the nanoconjugate. The in vitro cytotoxicity is carried out in VERO cell lines and anti cancer study in cervical cancer HeLa cell lines. Further, the gene expression of one of the apoptotic molecule, Caspase was screened. The nanocarrier showed anti cancer activity in treated cervical cancer HeLa cell lines and as well as commendable non toxicity in treated normal vero cell lines. On this positive note, this nanocarrier study could be further expanded.
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Acknowledgments
I would like to acknowledge Dr. Sathish Kota, Scientist, TRPVB, TANUVAS, Chennai and Dr. Harini Challa, Post Doctoral Fellow, TRPVB, TANUVAS, Chennai for their courteous assistance all through the in vitro studies.
Funding
Funding: This work was funded by the TNSCST (TamilNadu State Council for Science and Technology) through RFRS (Research Funding for Research Scholars) – (2016–2018)
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Kamala Priya, M.R., Iyer, P.R. (2019). Apoptotic Activity in Cervical Cancer HeLa Cell Lines Treated with Chitosan Nanoconjugated Drug Doxorubicin – as Nanocarrier for Drug Delivery. In: Rajan, M., Anand, K., Chuturgoon, A. (eds) Proceedings of the International Conference on Nanomedicine (ICON-2019). ICON 2019. Springer Proceedings in Materials. Springer, Cham. https://doi.org/10.1007/978-3-030-25135-2_5
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DOI: https://doi.org/10.1007/978-3-030-25135-2_5
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