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Genetic Transformation of Salvia miltiorrhiza

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Book cover The Salvia miltiorrhiza Genome

Part of the book series: Compendium of Plant Genomes ((CPG))

Abstract

Salvia miltiorrhiza is a valuable traditional Chinese medicinal herb. Due to the great importance of tanshinones and phenolic acids in S. miltiorrhiza, it is necessary to increase their yields to satisfy the rapidly growing demand. Many efforts have been made to elucidate the biosynthetic pathways of tanshinones and phenolic acids. This chapter describes two Agrobacterium-mediated S. miltiorrhiza transformation systems, named Agrobacterium rhizogenes-mediated hairy root transformation, and Agrobacterium tumefaciens-mediated ‘composite’ plant transformation, respectively. These systems provide a solid foundation for genetic engineering of S. miltiorrhiza to enhance these bioactive compounds. The achievements of S. miltiorrhiza metabolic engineering obtained through transformation are summarized. In addition, recent advances on the application of activation tagging mutagenesis (ATM) and CRISPR/Cas9 for mutation of genes in S. miltiorrhiza are discussed.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (81872964, 81773836) and the CAMS Innovation Fund for Medical Sciences (CIFMS) (2016-I2M-3-016).

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  1. Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College, No. 151 Malianwa North Road, Haidian District, Beijing, 100193, China

    Meizhen Wang & Shanfa Lu

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  1. Meizhen Wang
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  2. Shanfa Lu
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Correspondence to Shanfa Lu .

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  1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China

    Shanfa Lu

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Wang, M., Lu, S. (2019). Genetic Transformation of Salvia miltiorrhiza. In: Lu, S. (eds) The Salvia miltiorrhiza Genome. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-030-24716-4_13

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