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Synthetic Seeds pp 113–154Cite as

Insight View of Topical Trends on Synthetic Seeds of Rare and Endangered Plant Species and Its Future Prospects

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Abstract

In the present era, global plant biodiversity is dilapidated annually at a pioneering rate. Preservation of plant genetic resources has been threatened by the safety with foremost problems by limiting its efficiency. Contemporary trends on synthetic seeds, i.e., artificially encapsulated somatic embryos, open up new avenue in agriculture. It is one of the most promising alternate tools for propagation of many rare and endangered plant species. Success of synthetic seeds endowed with its protective hydrogel coating by increasing the growth of micropropagule in the field conditions. Synthetic seeds are also helpful in stipulations of their role in preventing the spread of plant diseases, and it is a gifted path for scale-up of multi-clone production for commercial purpose. Synthetic seeds are more durable for handling, transportation, and storage. Presently, it is well documented that any kind of non-embryogenic explants from tissue culture can be used for synthetic seed production like shoot tips, axillary buds, nodal segments, protocorms, bulblets, section of callus, bipolar propagule, or unipolar propagule. Currently, significant focus has been given for synthetic seeds, as they are economical for conservation of germplasm, maintenance of the genetic uniformity of plants through clonal propagation, and direct deliverance to the field. Under this context, various effective protocols for preparing synthetic seeds and conservation of plants are developed. Accordingly, an appraisal on synthetic seed aspects of various endangered and rare plant species has been contemplated, and the same will be covered in this chapter.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

BAP:

6-Benzylaminopurine

Ca(NO3)2 :

Calcium nitrate

CaCl2 :

Calcium chloride

IAA:

Indole-3-acetic acid

MS:

Murashige and Skoog medium

NAA:

α-Naphthalene acetic acid

PLBs:

Protocorm-like bodies

TDZ:

Thidiazuron

TTC:

Triphenyltetrazolium chloride

References

  • Abraham V (1981) Introduction to orchids. Tropical Botanic Garden and Research Institute, Trivandrum, pp 370–371

    Google Scholar 

  • Aewsakul N, Maneesorn D, Serivichyaswat P, Taluengjit A, Nontachaiyapoom S (2013) Ex vitro symbiotic seed germination of Spathoglottis plicata Blume. on common orchid cultivation substrates. Sci Hortic 160:238–242

    Article  Google Scholar 

  • Alcaraz F, Garre M, MartõÂnez JM, Peinado M (1987) Centaurium rigualii Esteve (Gentianaceae). In: GoÂmez-Campo C (ed) Libro Rojo de las Especies Vegetales Amenazadas de EspanÄa Peninsular e Islas Baleares. ICONA, Madrid, p 188±9

    Google Scholar 

  • Algabri AA, Pandhure N (2017) In vitro multiplication studies in Spilanthes acmella L. (Murr.). Int J Sci Res 6:993–994

    Google Scholar 

  • Amiri ME (2005) In vitro techniques to study the shoot-tip grafting of Prunus avium L. (cherry) var. J Food Agric Environ 4:151–154

    Google Scholar 

  • Anonymous (1976) Wealth of India: a dictionary of Indian raw materials and industrial products, Publication and information directorate, vol X. CSIR, New Delhi, pp 44–47

    Google Scholar 

  • Anonymous (1985) The wealth of India – raw materials, vol IA. Council of Scientific and Industrial Research (CSIR), New Delhi

    Google Scholar 

  • Anonymous (2003) The wealth of India. A dictionary of Indian raw materials and industrial products. Council of Scientific and Industrial Research, New Delhi, pp 398–399

    Google Scholar 

  • Ara H, Jaiswal U, Jaiswal VS (2000) Synthetic seed: prospects and limitations. Curr Sci 78:1438–1444

    Google Scholar 

  • Arghavani P, Haghbeen K, Mousavi A (2015) Enhancement of shikalkin production in Arnebia euchroma callus by a fungal elicitor, Rhizoctonia solani. Iran J Biotechnol 13:10–16

    Article  PubMed  PubMed Central  Google Scholar 

  • Arya HC, Shekhawat NS (1986) Clonal multiplication of tree species in Thar desert through tissue culture. Forest Ecol Manage 16:201–208

    Article  CAS  Google Scholar 

  • Asghar S, Ahmad T, Hafiz IA, Yaseen M (2011) In vitro propagation of orchid (Dendrobium nobile) var. Emma white. Afr J Biotechnol 10:3097–3103

    Article  CAS  Google Scholar 

  • Aslam M, Singh R, Negi PS, Bhakuni DS, Das SC (2006) Enhanced in vitro regeneration from cotyledonary node explants of Tecomella undulata (Smith) Seem. Proc Nat Acad Sci India Sec B: Biol Sci 76:281–285

    CAS  Google Scholar 

  • Ata A, Iverson CD, Kalhari KS, Akhter S, Betteridge J, Meshkatalsadat MH, Orhan I, Sener B (2010) Triterpenoidal alkaloids from Buxus hyrcana and their enzyme inhibitory, anti-fungal and anti-leishmanial activities. Phytochemistry 71:1780–1786

    Article  CAS  PubMed  Google Scholar 

  • Babar ZU, Ata A, Meshkatalsadat MH (2006) New bioactive steroidal alkaloids from Buxus hyrcana. Steroids 71:1045–1051

    Article  CAS  PubMed  Google Scholar 

  • Badola HK, Pal M (2002) Endangered medicinal plant species in Himachal Pradesh. Curr Sci 83:797–798

    Google Scholar 

  • Bais HP, Green JB, Walkerc TS, Okemo PO, Vivanco JM (2002) In vitro propagation of Spilanthes mauritiana DC., an endangered medicinal herb, through axillary bud cultures. In vitro Cell Dev Biol Plant 38:598–601

    Article  CAS  Google Scholar 

  • Balaraju K, Agastian P, Ignacimuthu S (2009) Micropropagation of Swertia chirata Buch.-Hams. ex Wall.: a critically endangered medicinal herb. Acta Physiol Plant 31:487–494

    Article  Google Scholar 

  • Balaraju K, Saravanan S, Agastian P, Ignacimuthu S (2011) A rapid system for micropropagation of Swertia chirata Buch-Ham. ex Wall.: an endangered medicinal herb via direct somatic embryogenesis. Acta Physiol Plant 33(4):1123–1133

    Article  Google Scholar 

  • Baskaran P, Kumari A, Van Staden J (2018) In vitro propagation via organogenesis and synthetic seeds of Urginea altissima (L.f.) Baker: a threatened medicinal plant. 3 Biotech 8:18

    Article  PubMed  Google Scholar 

  • Bekheet SA (2006) A synthetic seed method through encapsulation of in vitro proliferated bulblets of garlic (Allium sativum L.). Arab J Biotechnol 9:415–426

    Google Scholar 

  • Bektas E, Sokmen A (2016) In vitro seed germination, plantlet growth, tuberization, and synthetic seed production of Serapias vomeracea (Burm.f.) Briq. Turk J Bot 40:584–594

    Article  CAS  Google Scholar 

  • Bentley R, Trimen H (1992) Medicinal plants. Allied Book Centre, Dehradun

    Google Scholar 

  • Bhansali R (1993) Bud culture for shoot multiplication and plantlet formation of Tecomella undulata (Rohida), a woody tree of the arid zone. Trop Sci 3:31–38

    Google Scholar 

  • Bhat AJ, Kumar M, Negi AK, Todaria NP (2013) Informants’ consensus on ethnomedicinal plants in Kedarnath Wild life Sanctuary of Indian Himalayas. J Med Plants Res 7:148–154

    Google Scholar 

  • Bhatt A, Rawal RS, Dhar U (2006) Ecological features of a critically rare medicinal plant, Swertia chirayita, in Himalaya. Plant Species Biol 21:49–52

    Article  Google Scholar 

  • Bhattacharyya P, Kumaria S (2015) Molecular characterization of Dendrobium nobile Lindl., an endangered medicinal orchid, based on randomly amplified polymorphic DNA. Plant Syst Evol 301:201–210

    Article  CAS  Google Scholar 

  • Bhattacharyya P, Van Staden J (2016) Ansellia africana (Leopard orchid): a medicinal orchid species with untapped reserves of important biomolecules: a mini review. S Afr J Bot 106:181–185

    Article  CAS  Google Scholar 

  • Bhattacharyya P, Kumaria S, Tandon P (2016) High frequency regeneration protocol for Dendrobium nobile: a model tissue culture approach for propagation of medicinally important orchid species. S Afr J Bot 104:232–243

    Article  CAS  Google Scholar 

  • Bhattacharyya P, Kumar V, Johannes VS (2017) Assessment of genetic stability amongst micropropagated Ansellia africana, a vulnerable medicinal orchid species of Africa using SCoT markers. S Afr J Bot 108:294–302

    Article  CAS  Google Scholar 

  • Bhattacharyya P, Kumar V, Van Staden J (2018) In vitro encapsulation based short term storage and assessment of genetic homogeneity in regenerated Ansellia africana (Leopard orchid) using gene targeted molecular markers. Plant Cell Tissue Organ Cult 133:299–310

    Article  CAS  Google Scholar 

  • Blumenthal M (1998) Plantain. In: Blumenthal M, Busse WR (eds) The complete german commission E monographs-therapeutic guide to herbal medicines. American Botanical Council in Cooperation with Integrative Medicine Communications, Boston, pp 107–123

    Google Scholar 

  • Borner A (2006) Preservation of plant genetic resources in the biotechnology era. Biotechnol J 1:1393–1404

    Article  PubMed  CAS  Google Scholar 

  • Britto SJ, Natarajan E, Arockiasamy DI (2003) In vitro flowering and shoot multiplication from nodal explants of Ceropegia bulbosa Roxb. var. bulbosa. Taiwania 48:106–111

    Google Scholar 

  • Budhiraja RD, Sudhir S, Garg KN (1983) Cardiovascular effects of a withanolide from Withania coagulans Dunal fruits. Indian J Physiol Pharmacol 27:129–134

    CAS  PubMed  Google Scholar 

  • Budhiraja RD, Bala S, Craeg FN, Arora B (1986) Protective effect of 3-beta-hydroxy-2-3 dihydro withanolide-F against CCl4 induced Hepatotoxicity. Planta Med 52:28–29

    Article  Google Scholar 

  • Capuano G, Piccioni E, Standardi A (1998) Effect of different treatments on the conversion of M. 26 apple rootstock synthetic seeds obtained from encapsulated apical and axillary micropropagated buds. J Hortic Sci Biotechnol 73:299–305

    Article  Google Scholar 

  • Chal J, Kumar V, Kaushik S (2011) A phytopharmacological overview on Tecomella undulata G. Don. J Appl Pharm Sci 1:11–12

    Google Scholar 

  • Chaudhuri KN, Ghosh B, Jha S (2004) The root: a potential new source of competent cells for high-frequency regeneration in Tylophora indica. Plant Cell Rep 10:731–740

    Google Scholar 

  • Chaudhuri KN, Ghosh B, Tepfer D, Jha S (2006) Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium rhizogenes. Plant Cell Rep 25:1059–1066

    Article  CAS  PubMed  Google Scholar 

  • Chaudhuri RK, Pal A, Jha TB (2007) Production of genetically uniform plants from nodal explants of Swertia chirata Buch.-Ham. ex Wall.—an endangered medicinal herb. In vitro Cell Dev Biol Plant 43:467–472

    Article  CAS  Google Scholar 

  • Chaudhuri RK, Pal A, Jha TB (2008) Conservation of Swertia chirata through direct shoot multiplication from leaf explants. Plant Biotechnol Rep 2:213–218

    Article  Google Scholar 

  • Chaudhuri RK, Pal A, Jha TB (2009) Regeneration and characterization of Swertia chirata Buch.-Ham. ex Wall. plants from immature seed cultures. Sci Hortic 120:107–114

    Article  CAS  Google Scholar 

  • Chavan JJ, Nimbalkar MS, Gaikwad NB, Dixit GB, Yadav SR (2011) In vitro propagation of Ceropegia spiralis Wight – an endemic and rare potential ornamental plant of peninsular India. Proc Nat Acad Sci India Sect B 81:1

    Google Scholar 

  • Chetia S, Deka PC, Devi J (1998) Germination of fresh and stored encapsulated protocorms of orchids. Indian J Exp Biol 136:108–111

    Google Scholar 

  • Chhajer S, Kalia RK (2017) Seasonal and micro-environmental factors controlling clonal propagation of mature trees of marwar teak (Tecomella undulata (Sm.) Seem). Acta Physiol Plant 39:60

    Article  CAS  Google Scholar 

  • Chiang LC, Lean TNG, Chiang W, Chang MY, Lin CC (2003) Immunomodulatory activities of flavonoids, monoterpenoids, triterpenoids, iridoid glycosides and phenolic compounds of Plantago species. Planta Med 69:600–604

    Article  CAS  PubMed  Google Scholar 

  • Chinsamy M, Finnie JF, Van Staden J (2011) The ethnobotany of South African medicinal orchids. S Afr J Bot 77:2–9

    Article  Google Scholar 

  • Chinsamy M, Finnie JF, Van Staden J (2014) Anti-inflammatory, antioxidant, anti-cholinesterase activity and mutagenicity of South African medicinal orchids. S Afr J Bot 91:88–98

    Article  Google Scholar 

  • Chithra M, Martin KP, Sunandakumari C, Madhusoodanan PV (2005) Somatic embryogenesis, encapsulation and plant regeneration of Rotula aquatica Lour. a rare rhoeophytic woody medicinal plant. In vitro Cell Dev Biol Plant 41:28–31

    Article  Google Scholar 

  • Choudhary MI, Shahnaz S, Parveen S, Khalid A, Mesaik MA, Ayatollahi SA, Atta-ur-Rahman (2006) New cholinesterase-inhibiting triterpenoid alkaloids from Buxus hyrcana. Chem Biodivers 3:1039–1052

    Article  CAS  PubMed  Google Scholar 

  • Chugh S, Guha S, Rao IU (2009) Micropropagation of Orchids: a review on the potential of different explants. Sci Hortic 122:507–520

    Article  CAS  Google Scholar 

  • Clarke CB (1885) Verbenaceae. In: Hooker JD (ed) The flora of British India, vol IV. L. Reeve, London, pp 560–604

    Google Scholar 

  • Coelho N, González-Benito ME, Romano A (2014a) Approaches for the cryopreservation of Plantago algarbiensis, a rare endemic species of the Algarve. Cryo Letters 35:521–529

    PubMed  Google Scholar 

  • Coelho N, Gonzalez-Benito ME, Romano A (2014b) Cryopreservation of shoot tips from the endangered endemic species Tuberaria major. Acta Physiol Plant 36:3333–3336

    Article  CAS  Google Scholar 

  • Cribb PJ, Tang CZ (1982) Spathoglottis (Orchidaceae) in Australia and the Pacific Islands. Kew Bull 36:721–729

    Article  Google Scholar 

  • Das PK, Sahoo S, Bal S (2008) Ethnobotanical studies on Orchids of Niyamgiri hill ranges, Orissa, India. Ethnobot Leaflets 12:70–78

    Google Scholar 

  • Datta KB, Kanjilal B, De Sarker D (1999) Artificial seed technology: development of a protocol in Geodorum densiflorum (Lam) Schltr. – an endangered orchid. Curr Sci 76:1142–1145

    Google Scholar 

  • Davydenkov VN, Patudin AV, Papov YG, Rabinovich SA, Miroshnikov AI (1991) Cell culture of Arnebia euchroma (Royle) Jonst. – novel source of shikonin production. Him Farm Zh 1:53–55

    Google Scholar 

  • Delforge P (1995) Orchids of Britain and Europe. Delachaux et Niestlé SA, Lausanne

    Google Scholar 

  • Devendra BN, Srinivas N, Naik GR (2011) Direct somatic embryogenesis and synthetic seed production from Tylophora indica (Burm.f.) Merrill an endangered, medicinally important plant. Int J Bot 7:216–222

    Article  CAS  Google Scholar 

  • Devi M, Sharma J, Sarma A (2000) In vitro culture of artificial seeds of Vanda coerulea – an endangered orchid. Res Crops 1:205–207

    Google Scholar 

  • Devi PS, Arundathi A, Raghava Rao T (2011) Multiple shoot induction and regeneration of whole plant from cotyledonary node and nodal explants of Sterculia urens. J Plant Biochem Biotechnol 20:161–165

    Article  Google Scholar 

  • Devi PS, Satyanarayana B, Arundathi A, Raghava Rao T (2012) Effect of storage temperature and dormancy-breaking treatments on seed germination, moisture content and seed vigor in gum karaya (Sterculia urens Roxb). Forest Sci Technol 8:1–5

    Article  Google Scholar 

  • Devi PS, Satyanarayana B, Arundhati A, Rao TR (2014) Plant regeneration from encapsulated nodal segments of Sterculia urens Roxb., an endangered gum-yielding tree. J Crop Sci Biotechnol 17:21–25

    Article  Google Scholar 

  • Dhir R, Shekhawat GS (2013) Production, storability and morphogenic response of alginate encapsulated axillary meristems and genetic fidelity evaluation of in vitro regenerated Ceropegia bulbosa: a pharmaceutically important threatened plant species. Ind Crop Prod 47:139–144

    Article  CAS  Google Scholar 

  • Divakaran M, Babu KN, Peter KV (2006) Conservation of Vanilla species. In vitro Sci Hortic 110(2):175–180

    Google Scholar 

  • Ebrahimzadeh MA, Nabavi SM, Nabavi SF, Bahramian F, Bekhradnia AR (2010) Antioxidant and free radical scavenging activity of H. officinalis L. var. angustifolius, V. odorata, B. hyrcana and C. speciosum. Pak J Pharm Sci 23:29–34

    CAS  PubMed  Google Scholar 

  • ECCB (1995) Red data book of European bryophytes. European Committee for Conservation of Bryophytes (ECCB), Trondheim

    Google Scholar 

  • Eeckhaut T, Janssens K, De Keyser E, Riek JD (2010) Micropropagation of Rhododendron. Methods Mol Biol (Clifton NJ) 589:141–152

    Article  CAS  Google Scholar 

  • El Grari R, Backhaus RA (1987) In vitro propagation of red squill, Urginea maritima Baker. Plant Cell Tissue Organ Cult 10:65–71

    Article  Google Scholar 

  • Esmaeili S, Naghibi F, Mosaddegh M, Sahranavard S, Ghafari S, Abdullah NR (2009) Screening of antiplasmodial properties among some traditionally used Iranian plants. J Ethnopharmacol 121:400–404

    Article  PubMed  Google Scholar 

  • Faisal M, Anis M (2003) Rapid mass propagation of Tylophora indica Merrill via leaf callus culture. Plant Cell Tissue Organ Cult 75:125–129

    Article  CAS  Google Scholar 

  • Faisal M, Anis M (2007) Regeneration of plants from alginate encapsulated shoots of Tylophora indica (Burm. f.) Merrill, an endangered medicinal plant. J Hortic Sci Biotechnol 82:351–354

    Article  CAS  Google Scholar 

  • Faisal M, Singh S, Anis M (2005) In vitro regeneration and plant establishment of Tylophora indica (Burm. f.) Merrill: petiole callus culture. In vitro Cell Dev Biol Plant 41:511–515

    Article  Google Scholar 

  • Faria RT, Illg RD (1995) Propagac¸ao clonal de hıbridos de Dendrobium nobile Lindl. Congr Bras Floric. Plantas Ornamentais, pp 40–41

    Google Scholar 

  • Faria RT, Rodrigues FN, Oliveira LVR, Muller C (2004) In vitro Dendrobium nobile plant growth and rooting in different sucrose concentrations. Hortic Bras, Brasília 22:780–783

    Article  Google Scholar 

  • Franco JA (1984) Nova Flora de Portugal (Continente e Ac¸ores), vol II. Sociedade Asto’ria, Lda, Lisboa

    Google Scholar 

  • Gangaprasad A, Decruse SW, Seeni S (1995) Species seedling and mericlone bottles for practical conservation and sustained economic utilization of the Western Ghats orchids. In: Proceedings of 7th Kerala Science Congress, Palakkad, Kerala, pp 27–30

    Google Scholar 

  • Gantait S, Sinniah UR (2013) Storability, post-storage conversion and genetic stability assessment of alginate-encapsulated shoot tips of monopodial orchid hybrid Aranda Wan Chark Kuan ‘Blue’ 9 Vanda coerulea Grifft. ex. Lindl. Plant Biotechnol Rep 7:257–266

    Article  Google Scholar 

  • Gantait S, Bustam S, Sinniah UR (2012) Alginate-encapsulation, short-term storage and plant regeneration from protocorm-like bodies of Aranda Wan Chark Kuan ‘Blue’ × Vanda coerulea Grifft. ex. Lindl. (Orchidaceae). Plant Growth Regul 68:303–311

    Article  CAS  Google Scholar 

  • Gantait S, Kundu S, Ali NM, Sahu NC (2015) Synthetic seed production of medicinal plants: a review on influence of explants, encapsulation agent and matrix. Acta Physiol Plant 7:98

    Article  CAS  Google Scholar 

  • Gantait S, Vijayan J, Majee A (2017) Artificial seed production of Tylophora indica for interim storing and swapping of germplasm. Hortic Plant J 3:41–46

    Article  Google Scholar 

  • Geetha S, Sudheer AS (2000) In vitro propagation of Vanilla planifolia, a tropical orchid. Curr Sci 79:886–889

    Google Scholar 

  • Geetha R, Gopal GV, Niranjan MH (2009) In vitro response of encapsulated shoot tips of Spilanthes acmella. J Basic Appl Biol 3:82–86

    Google Scholar 

  • Giridhar P, Ravishankar GA (2004) Efficient micropropagation of Vanilla planifolia Andr. under influence of thidiazuron, zeatin and coconut milk. Indian J Biotechnol 3:113–118

    CAS  Google Scholar 

  • Giridhar P, Reddy BO, Ravishankar GA (2001) Silver nitrate influences in vitro shoot multiplication and root formation in Vanilla planifolia Andr. Curr Sci 81:1166–1170

    CAS  Google Scholar 

  • GIROS (2009) Orchidee d’Italia. Castello srl, Milano

    Google Scholar 

  • Goncalves S, Martins N, Romano A (2009) Micropropagation and conservation of endangered species Plantago algarbiensis and P. almogravensis. Biol Plant 53:774–778

    Article  Google Scholar 

  • Goncalves S, Fernandes L, Perez-Garcıa F, Gonzalez-Benito ME, Romano A (2009a) Germination requirements and cryopreservation tolerance of seeds of the endangered species Tuberaria major. Seed Sci Technol 37:480–484

    Article  Google Scholar 

  • Goncalves S, Fernandes L, Romano A (2010) High-frequency in vitro propagation of the endangered species Tuberaria major. Plant Cell Tissue Organ Cult 101:359–363

    Article  Google Scholar 

  • Gonzalez ML, Mallon R, Reinoso J, Rodriguez-Oubina J (2006) In vitro micropropagation and long-term conservation of the endangered moss Splachnum ampullaceum Hedw. Biol Plant 50:339–345

    Article  CAS  Google Scholar 

  • Gonzalez-Arnao MT, Lazaro-Vallejo CE, Engelmann F, Gamez-Pastrana R, Martinez-Ocampo YM, Pastelin-Solano MC, Diaz-Ramos C (2009) Multiplication and cryopreservation of vanilla (Vanilla planifolia ‘Andrews’). In Vitro Cell Dev Biol-Plant 45(5):574–582

    Article  Google Scholar 

  • Gonzalez-Benito ME, Perez C, Viviani AB (1997) Cryopreservation of nodal explants of an endangered plant species (Centaurium rigualii Esteve) using the encapsulation-dehydration method. Biodivers Conserv 6:583–590

    Article  Google Scholar 

  • Gopalakrishnan C, Shankaranarayan D, Nazimudeen SK, Kameshwaran L (1980) Effect of tylophorine; a major alkaloid of Tylophora indica, on immunopathological and inflammatory reactions. Indian J Med Res 71:940–948

    CAS  PubMed  Google Scholar 

  • Goyal D, Bhadauria S (2006) In vitro propagation of Ceropegia bulbosa using nodal segments. Indian J Biotechnol 5:565–567

    CAS  Google Scholar 

  • Gupta RC, Ansari MS, Kapoor LD (1970) Pharmacognostical studies on Jivanti Part I-Desmotrichum fimbriatum Blume. Bull Bot Surv India 12:20–36

    Google Scholar 

  • Gupta K, Garg S, Singh J, Kumar M (2014) Enhanced production of napthoquinone metabolite (shikonin) from cell suspension culture of Arnebia sp. and its up-scaling through bioreactor. 3 Biotech 4(3):263–273

    Article  PubMed  Google Scholar 

  • Haque SM, Ghosh B (2017) Regeneration of cytologically stable plants through dedifferentiation, redifferentiation and artificial seeds in Spathoglottis plicata Blume. (Orchidaceae). Hortic Plant J 3(5):199–208

    Article  Google Scholar 

  • Haw AB, Keng CL (2003) Micropropagation of Spilanthes acmella L., a bio-insecticide plant, through proliferation of multiple shoots. J Appl Hortic 5(2):65–68

    Google Scholar 

  • Honek A, Martinkova Z, Saska P (2005) Post-dispersal predation of taraxacum officinale (dandelion) seed. J Ecol 93:345–352

    Article  Google Scholar 

  • Hossain MM, Dey R (2013) Multiple regeneration pathways in Spathoglottis plicata Blume—a study in vitro. S Afr J Bot 85:56–62

    Article  CAS  Google Scholar 

  • Hossain MM, Sharma M, Pathak P (2009) Cost effective protocol for in vitro mass propagation of Cymbidium aloifolium (L.) Sw.-a medicinally important orchid. Eng Life Sci 9(6):444–453

    Article  CAS  Google Scholar 

  • Hrahsel L, Thangjam R (2015) Asymbiotic in vitro seed germination and regeneration of Vanda coerulea Giff. Ex. Lindl., an endangered orchid from Northeast India. J Plant Sci Res 2:1–5

    Google Scholar 

  • Hussain TM, Chandrasekhar T, Gopal GR (2008) Micropropagation of Sterculia urens Roxb., an endangered tree species from intact seedlings. Afr J Biotechnol 7:95–101

    CAS  Google Scholar 

  • Hutchings A (1989) A survey and analysis of traditional medicinal plants as used by the Zulu, Xhosa and Sotho. Bothalia 19:111–123

    Google Scholar 

  • Hutchings A, Scott H, Lewis G, Cunningllam AB (1996) Zulu medicinal plants-an inventory. University of Natal Press, Pietermaritzburg

    Google Scholar 

  • ICN (Instituto da Conservação da Natureza) (2006) Plano Sectorial da Rede Natura. Flora: Tuberaria major (Willk.) P. Silva and Rozeira. http://www.icnf.pt/portal/naturaclas/rn2000/resource/rnplan- set/flora/tub-maj

  • ICN (Instituto de Conservação da Natureza) (2007) Plano nacional de conservação da flora em perigo (1a fase), Relato’rio final do Projecto Life—Natureza III P\8480, vol IV. Instituto da Conservação da Natureza, Lisboa

    Google Scholar 

  • Jain SK, Defillips R (1991) Asclepidaceae. In: Algonae MI (ed) Medicinal plants of India. Reference, Michigan, pp 144–152

    Google Scholar 

  • Jana S, Shekhawat GS (2011) Plant growth regulators, adenine sulfate and carbohydrates regulate organogenesis and in vitro flowering of Anethum graveolens. Acta Physiol Plant 33:305–311

    Article  CAS  Google Scholar 

  • Janarthanam B, Seshadri S (2008) Plantlet regeneration from leaf derived callus of Vanilla planifolia Andr. In vitro Cell Dev Biol Plant 44:84–89

    Article  CAS  Google Scholar 

  • Jayanthi M, Mandal PK (2001) Plant regeneration through somatic embryogenesis and RAPD analysis of regenerated plants in Tylophora indica (Burm. f. Merrill.). In vitro Cell Dev Biol Plant 37:576–580

    Article  CAS  Google Scholar 

  • Jha S, Sen S (1986) Mass propagation of Indian squill, Urginea indica Kunth in vitro. Acta Hortic 188:105–109

    Article  Google Scholar 

  • Jha S, Mitra GC, Sen S (1984) In vitro regeneration from bulb explants of Indian squill, Urginea indica Kunth. Plant Cell Tissue Organ Cult 3:91–100

    Article  Google Scholar 

  • Jha BT, Dafadar A, Chaudhari KR (2011) Somatic embryogenesis in Swertia chirata Buch.-Ham. ex Wall. – a multipotent medicinal herb. Asian J Biotechnol 3:186–193

    Article  CAS  Google Scholar 

  • Jiang B, Yang YG, Guo YM, Guo ZC, Chen YZ (2005) Thidiazuron-induced in vitro shoot organogenesis of the medicinal plant Arnebia euchroma (Royle) Johnst. In vitro Cell Dev Biol Plant 41:677–681

    Article  CAS  Google Scholar 

  • Jitsopakul N, Thammasiri K, Ishikawa K (2008) Cryopreservation of Vanda coerulea protocorms by encapsulation-dehydration. Cryo Letters 29:253–260

    PubMed  Google Scholar 

  • Joshi P, Dhawan V (2005) Swertia chirayita – an overview. Curr Sci 89:635–640

    CAS  Google Scholar 

  • Joshi P, Dhawan V (2007) Axillary multiplication of Swertia chirayita (Roxb. Ex Fleming) H. Karst., a critically endangered medicinal herb of temperate Himalayas. In vitro Cell Dev Biol Plant 43:631–638

    Article  CAS  Google Scholar 

  • Joshi V, Tiwari KL, Jadhav SK (2015) In vitro propagation of Spilanthes acmella (L.) Murray using semisolid and liquid medium. Indian J Biotechnol 14:112–116

    CAS  Google Scholar 

  • Joshi H, Nekkala S, Soner D, Kher MM, Nataraj M (2016) In vitro Shoot Multiplication of Withania coagulans (Stocks) Dunal. Plant Tissue Cult Biotechnol 26:187–195

    Article  Google Scholar 

  • Kaith BS, Kaith NS, Chauhan NS (1996) Antiinflammatory effect of Arnebia euchroma root extracts in rats. J Ethnopharmacol 55:77–80

    Article  CAS  PubMed  Google Scholar 

  • Kalimuthu K, Prabakaran R (2013) In vitro flowering from nodal explants of Ceropegia pusilla Wight and Arn. Int J Bot Res 3:35–42

    Google Scholar 

  • Kamińska M, Skrzypek E, Wilmowicz E, Tretyn A, Trejgell A (2016) Effect of light condition and ABA on cold storage and post-storage propagation of Taraxacum pieninicum. Plant Cell Tissue Organ Cult 127:25–34

    Article  CAS  Google Scholar 

  • Kamińska M, Gołębiewski M, Tretyn A, Trejgell A (2018a) Efficient long-term conservation of Taraxacum pieninicum synthetic seeds in slow growth conditions. Plant Cell Tissue Organ Cult 132:469–478

    Article  CAS  Google Scholar 

  • Kamińska M, Tretyn A, Trejgell A (2018b) Effect of jasmonic acid on cold-storage of Taraxacum pieninicum encapsulated shoot tips. Plant Cell Tissue Organ Cult 135:487–497

    Article  CAS  Google Scholar 

  • Karuppusamy S, Kiranmai C, Aruna V, Pullaiah T (2009) In vitro conservation of Ceropegia intermedia-an endemic plant of south India. Afr J Biotechnol 8:4052–4057

    CAS  Google Scholar 

  • Kauth PJ, Johnson TR, Stewart SL, Kane ME (2008) A classroom exercise in hand pollination and in vitro asymbiotic orchid seed germination. Plant Cell Tissue Organ Cult 93:223–230

    Article  Google Scholar 

  • Kaviani B, Negahdar N (2017) Propagation, micropropagation and cryopreservation of Buxus hyrcana Pojark., an endangered ornamental shrub. S Afr J Bot 111:326–335

    Article  CAS  Google Scholar 

  • Khor E, Ng WF, Loh CS (1998) Two-coat systems for encapsulation of Spathoglottis plicata (Orchidaceae) seeds and protocorms. Biotechnol Bioeng 59:635–639

    Article  CAS  PubMed  Google Scholar 

  • Kirtikar KR, Basu BD (1975) In: Singh B, Singh MP (eds) Indian medicinal plants, vol 3. International Book Distribution, Dehradun

    Google Scholar 

  • Kirtikar KR, Basu BD (1984) Indian medicinal plants, vol III. LM Basu, Allahabad

    Google Scholar 

  • Kokwaro JO (1976) Medicinal plants of East Africa. East African Literature Bureau, Kampala, p 384

    Google Scholar 

  • Kondamudi R, Murthy KSR (2011) Micropropagation and in vitro flowering of Ceropegia pusilla. J Trop Med Plant 12:41–47

    Google Scholar 

  • Kosger HH, Ozturk M, Sokmen A, Bulut ESA (2009) Wound healing effects of Arnebia densiflora root extracts on rat palatal mucosa. Eur J Dent 3:96–99

    Article  PubMed  PubMed Central  Google Scholar 

  • Krishnareddy PV, Karuppusamy S, Pullaiah T (2011) In vitro propagation of Ceropegia juncea Roxb. Afr J Plant Sci 3:345–357

    Google Scholar 

  • Kumar V (2016) Gum karaya (Sterculia urens Roxb.): a potential gum tree. Van Sangyan 3:34–39

    Google Scholar 

  • Kumar V, Chandra S (2014) High frequency somatic embryogenesis and synthetic seed production of the endangered species Swertia chirayita. Biol Sect Bot 69(2):186–192

    CAS  Google Scholar 

  • Kumar V, Desai B (2016) Biodiversity and phytosociological analysis of plants around the Chikhali Taluka, Navsari district, Gujarat, India, vol 10. Ecoscan, pp 1–18

    Google Scholar 

  • Kumar CS, Manilal KS (1987) A synopsis of Ipsea (Orchidaceae). Kew Bull 42:937–943

    Article  Google Scholar 

  • Kumar CS, Shetty BV, Bennet SSR, Rao TA, Molur S, Walker S (2001) Conservation assessment and management plan workshop report for endemic orchids of the Western Ghats 2001. Wildlife information liaison development society zoo outreach organization, Coimbatore

    Google Scholar 

  • Kumar S, Singh KK, Rai LK (2004) In vitro propagation of an endangered Sikkim Himalayan rhododendron (R. maddeni) from cotyledonary nodal segments. J Am Rhododendrons Soc 58:101–105

    Google Scholar 

  • Kumar V, Singh SK, Bandopadhyay R, Sharma MM, Chandra S (2014) In vitro organogenesis secondary metabolite production and heavy metal analysis in Swertia chirayita. Cent Eur J Biol 9:686–698

    CAS  Google Scholar 

  • Kumar RR, Purohit VK, Prasad P, Nautiyal AR (2018) Efficient in vitro propagation protocol of Swertia chirayita (Roxb. ex Fleming) Karsten: a critically endangered medicinal plant. Natl Acad Sci Lett 41:123–127

    Article  CAS  Google Scholar 

  • Kumari S, Singh N (2012) Multiplication of desert teak Tecomella undulata under in vitro conditions. J Trop Med Plant 13:137–143

    Google Scholar 

  • Kumari P, Kumar V, Chandra S (2014) Synthetic seeds: a boon for conservation and exchange of germplasm. BMR Biotechnol 1:1–11

    Google Scholar 

  • Knudson L (1950) Germination of seeds of vanilla. Am J Bot 37(3):241–247

    Article  Google Scholar 

  • Kurian E, Thomas TD (2015) Multiple shoot induction from nodal segments of Spilanthes acmella (L.) Murr., a herbal pesticidal plant. STARS: Int J (Sci) 5:1–9

    Google Scholar 

  • Lambardi M, Benelli C, Ozudogru EA, Ozden-Tokatli Y (2006) Synthetic seed technology in ornamental plants. Floricult Ornam Plant Biotechnol 2:347–354

    Google Scholar 

  • Langat LC, Westchnig W, Langat MK, Mulholland DA (2013) Novel bufadienolides from South African Urginavia altissima (Hyacinthaceae). In: Proceedings of ICNP, p 213

    Google Scholar 

  • Leach DG (1961) Rhododendrons of the world. Charles Scribners’s Sons, New York, p 544

    Google Scholar 

  • Lee-Espinosa HE, Murguía-González J, García-Rosas B, Córdova-Contreras AL, Laguna-Cerda A, Mijangos-Cortés JO, Barahona-Pérez LF, Iglesias-Andreu LG, Santana-Buzzy N (2008) In vitro clonal propagation of Vanilla (Vanilla planifolia ‘Andrews’). Hort Science 43:454–458

    Article  Google Scholar 

  • Leng TC, Ping NS, Lim BP, Keng CL (2011) Detection of bioactive compounds from Spilanthes acmella (L.) plants and its various in vitro culture products. J Med Plant Res 5:371–378

    CAS  Google Scholar 

  • Lubinsky P (2003) Conservation of wild vanilla. In: Abstract of the first international congress of the Vanilla business, Vanilla 2003. Jersey N, USA, 11–12 November

    Google Scholar 

  • Mabberley DJ (1987) The plant book. Cambridge University Press, Cambridge

    Google Scholar 

  • Malabadi RB, Mulgund GS, Kallappa N (2005) Micropropagation of Dendrobium nobile from shoot tip sections. J Plant Physiol 162:473–478

    Article  CAS  PubMed  Google Scholar 

  • Malik S, Sharma M, Ahuja PS (2016) An efficient and economic method for in vitro propagation of Arnebia euchroma using liquid culture system. Am J Biotechnol Med Res 1:19–25

    Article  Google Scholar 

  • Mallon R, Barros P, Luzardo A, Gonzalez M (2007) Encapsulation of moss buds: an efficient method for the in vitro conservation and regeneration of the endangered moss Splachnum ampullaceum. Plant Cell Tissue Organ Cult 88:41–49

    Article  Google Scholar 

  • Maneerattanarungroj P, Bunnag S, Monthatong M (2007) In vitro conservation of Cleisostoma areitinum (Rchb.f.) Garay, rare Thai orchid species by an encapsulation-dehydration method. Asian J Plant Sci 6:1235–1240

    Article  CAS  Google Scholar 

  • Manilal KS, Kumar CS (1983) Rediscovery of Ipsea malabarica Hook.f. – an endemic orchid species from Silent Valley, Kerala. Bull Pure Appl Sci Res 2C:38–41

    Google Scholar 

  • Manjkhola S, Dhar U, Joshi M (2005) Organogenesis, Embryogenesis and synthetic seed production in Arnebia euchroma – a critically endangered medicinal plant of the Himalaya. In vitro Cell Dev Biol Plant 41:244–248

    Article  Google Scholar 

  • Manners V, Kumaria S, Tandon P (2010) Micropropagation of Vanda coerulea Griff ex Lindl.: a study of regeneration competence of roots in vitro. In: International conference on environmental engineering and applications (ICEEA 2010), pp 100–102

    Google Scholar 

  • Martin KP (2003a) Clonal propagation, encapsulation and reintroduction of Ipsea malabarica (Reichb. f.) J. D. Hook., an endangered orchid. In vitro Cell Dev Biol Plant 39:322–326

    Article  CAS  Google Scholar 

  • Martin K (2003b) Rapid in vitro multiplication and ex vitro rooting of Rotula aquatica Lour., a rare rhoeophytic woody medicinal plant. Plant Cell Rep 21:415–420

    Article  CAS  PubMed  Google Scholar 

  • Martin KP, Madassery J (2005) Rapid in vitro propagation of the threatened endemic orchid, Ipsea malabarica (Reichb.f.) J D Hook through protocorm-like bodies. Ind J Exp Bioly 43:829–834

    Google Scholar 

  • Martin KP, Pradeep AK (2003) Simple strategy for the in vitro conservation of Ipsea malabarica an endemic and endangered orchid of the Western Ghats of Kerala. India Plant Cell Tissue Organ Cult 74:197–200

    Article  Google Scholar 

  • Mascarenhas AF, Muralidhan EM (1989) Tissue culture of forest tree in India. Curr Sci 58:606–612

    Google Scholar 

  • Mathur S, Shekhawat GS, Batra A (2002a) Micropropagation of Salvadora persica via cotyledonary nodes. J Biotechnol 1:197–200

    CAS  Google Scholar 

  • Mathur S, Shekhawat GS, Batra A (2002b) An efficient in vitro method for mass propagation of Salvadora persica via apical meristems. J Biochem Biotechnol 11:125–127

    CAS  Google Scholar 

  • Mathur S, Shekhawat GS, Batra A (2008) Somatic embryogenesis and plantlet regeneration from cotyledon explants of Salvadora persica L. Phytomorphology 58:57–63

    Google Scholar 

  • McCartan SA, Van Staden J (1999) Micropropagation of members of the Hyacinthaceae with medicinal and ornamental potential – a review. S Afr J Bot 65:361–369

    Article  Google Scholar 

  • Medhi RP, Chakrabarti S (2009) Traditional knowledge of NE people on conservation of wild orchids. Indian J Tradit Knowl 8:11–16

    Google Scholar 

  • Mercure EW, Jones CS, Brand MH, Auer CA (1998) Anatomy of shoots and tumors of in vitro habituated Rhododendron Montego (Ericaceae) culture with tissue proliferation. Am J Bot 85:616–628

    Article  CAS  PubMed  Google Scholar 

  • Mesaik MA, Halim SA, Ul-Haq Z, Choudhary MI, Shahnaz S, Ayatollahi SAM, Murad S, Ahmad A (2010) Immunosuppressive activity of buxidin and Ebuxenone from Buxus hyrcana. Chem Biol Drug Des 75:310–317

    Article  CAS  PubMed  Google Scholar 

  • Micheli M, Hafiz IA, Standardi A (2007) Encapsulation of in vitro derived explants of olive (Olea europaea L. cv. Moraiolo), II. Effects of storage on capsule and derived shoots performance. Sci Hortic 113:286–292

    Article  Google Scholar 

  • Miyazawa M, Shimamura H, Nakamura S, Kameoka H (1997) Antimutagenic activity of gigantol from Dendrobium nobile. J Agric Food Chem 45:2849–2853

    Article  CAS  Google Scholar 

  • Mohanty P, Das MC, Kumaria S, Tandon P (2012) High-efficiency cryopreservation of the medicinal orchid Dendrobium nobile Lindl. Plant Cell Tissue Organ Cult 109:297–305

    Article  CAS  Google Scholar 

  • Mohanty P, Nongkling P, Das MC, Kumaria S, Tandon P (2013) Short-term storage of alginate-encapsulated protocorm-like bodies of Dendrobium nobile Lindl.: an endangered medicinal orchid from North-east India. 3 Biotech 3:235–239

    Article  PubMed  Google Scholar 

  • Molur S, Walker S (1998) Report of the workshop ‘Conservation assessment and management plan for selected medicinal plants species of northern, northeastern and central India’ (BCPP Endangered Species Project). Coimbatore, India. Zoo Outreach Organization; Conservation Breeding Specialist Group

    Google Scholar 

  • Morwal G, Jadhav SJ, Shinde A, Mandge N, Mandge N (2015) Conservation of Vanilla Planifolia by In-vitro micropropagation method. In: International journal of research in advent technology. Special issue national conference “ACGT 2015”, pp 13–14

    Google Scholar 

  • Murthy SRK (2011) Micropropagation and in vitro flowering of Ceropegia pusilla Wt. & Arn. J Trop Med Plan 12:41–48

    Google Scholar 

  • Murthy KSR, Kondamudi R, Pullaiah T (2010) High frequency somatic embryogenesis in Ceropegia spiralis Wight. – an endemic and endangered medicinal plant. Indian J Biotechnol 9:414–418

    CAS  Google Scholar 

  • Murthy KSR, Kondamudi R, Reddy MC, Karuppusamy S, Pullaiah T (2012) Check-list and conservation strategies of the genus Ceropegia in India. Int J Biodivers Conserv 4:304–315

    Google Scholar 

  • Murthy KSR, Reddy MC, Kondamudi R (2013) Synthetic seeds – a novel approach for the conservation of endangered C. spiralis wt. and C. pusilla. Bangladesh J Sci Ind Res 48:39–42

    Article  CAS  Google Scholar 

  • Nadkarni AK (1954) Indian materia medica, vol 1, 3rd edn. Popular Book Depot, Bombay

    Google Scholar 

  • Nadkarni KM (1976) Indian materia medica. Popular Prakashan, Bombay, pp 303–304

    Google Scholar 

  • Nagananda GS, Satishchandra N (2013) Antimicrobial activity of cold and hot successive pseudobulb extracts of Flickingeria nodosa (Dalz.) Seidenf. Pak J Biol Sci 16:1189–1193

    Article  CAS  PubMed  Google Scholar 

  • Nagananda GS, Satishchandra N, Rajath S (2011) Regeneration of encapsulated protocorm like bodies of medicinally important vulnerable orchid Flickingeria nodosa (Dalz.) Seidenf. Int J Bot 7:310–313

    Article  CAS  Google Scholar 

  • Nandwani D, Mathur N, Ramawat KG (1995) In vitro shoot multiplication from cotyledonary node explants of Tecomella undulata. Gartenbauwissenschaft 60:65–68

    CAS  Google Scholar 

  • Nandwani D, Sharma R, Ramawat KG (1996) High frequency regeneration in callus cultures of a tree Tecomella undulata. Gartenbauwissenschaft 61:147–150

    CAS  Google Scholar 

  • Nayak NR, Rath SP, Patnaik S (1997) In vitro propagation of three epiphytic orchids, Cymbidium aloifolium (L.) Sw., Dendrobium aphyllum (Roxb.) Fisch. and Dendrobium moschatum (Buch-Ham) Sw. through thidiazuron-induced high frequency shoot proliferation. Sci Hortic 71:243–250

    Article  Google Scholar 

  • Ng TB, Liu J, Wong JH, Ye X, Wing Sze SC, Tong Y, Zhang KY (2012) Review of research on Dendrobium, a prized folk medicine. Appl Microbiol Biotechnol 93(5):1795–1803

    Article  CAS  PubMed  Google Scholar 

  • Nikam TD, Savanth RS, Parage RS (2008) Micropropagation of Ceropegia hirsute Wt. & Arn., a starchy tuberous asclepiad. Indian J Biotechnol 7:129–132

    CAS  Google Scholar 

  • Nongdam P, Chongtham N (2011) In vitro rapid propagation of Cymbidium aloifolium (L.) Sw.: a medicinally important orchid via seed culture. J Biol Sci 11(3):254–260

    Article  CAS  Google Scholar 

  • Novak SD, Whitehouse AG (2013) Auxin regulates first leaf development and promotes the formation of protocorm, trichomes and rhizome-like structures in developing seedlings of Spathoglottis plicata (Orchidaceae). AoB Plants 5:pls053

    Article  PubMed Central  CAS  Google Scholar 

  • Nyende AB, Schittenhelm S, Wagner GM, Greef JM (2003) Production, storability, and regeneration of shoot tips of potato (Solanum tuberosum L.) encapsulated in calcium alginate hollow beads. In Vitro Cell Dev Biol Plant 39:540–544

    Article  Google Scholar 

  • Oliveira SOD, Sayd RM, Balzon TA, Scherwinski-Pereira JE (2013) A new procedure for in vitro propagation of vanilla (Vanilla planifolia) using a double-phase culture system. Sci Hortic 161:204–209

    Article  CAS  Google Scholar 

  • Orhan IE, Sinem AE, Fatma SS, Murat KBS (2012) Exploration of cholinesterase and tyrosinase inhibitory, antiprotozoal and antioxidant effects of Buxus sempervirens L. (boxwood). Ind Crop Prod 40:116–121

    Article  CAS  Google Scholar 

  • Oudhia P (2005) Medicinal herbs of Chhattisgarh, India having less known traditional uses 72 Roheda (Tecomella undulata family Bignoniaceae). (Online) Available from: http://botanical.com/site/column_poudhia/articles/449 roheda.html

  • Pandey V, Agrawal V (2009) Efficient micropropagation protocol of Spilanthes acmella L. possessing strong antimalarial activity. In vitro Cell Dev Biol Plant 45(4):491–499

    Article  Google Scholar 

  • Pant B, Raskoti BB (2013) Medicinal orchids of Nepal. Himalayan Map House, Nepal

    Google Scholar 

  • Pant M, Bisht P, Gusain MP (2010a) De novo shoot organogenesis from cultured root explants of Swertia chirata Buch.-Ham.ex Wall.: an endangered medicinal plant. Nat Sci 8:244–252

    Google Scholar 

  • Pant M, Bisht P, Gusain MP (2010b) In vitro propagation through axillary bud culture of Swertia chirata Buch. -Ham. ex Wall.: an endangered medicinal plant. Int J Integr Biol 10:49–53

    Google Scholar 

  • Papenfus HB, Naidoo D, Pošta M, Finnie JF, Van Staden J (2016) The effects of smoke derivatives on in vitro seed germination and development of the Leopard orchid Ansellia africana. Plant Biol 18:289–294

    Article  CAS  PubMed  Google Scholar 

  • Parveen S, Shahzad A (2014) Encapsulation of nodal segments of Cassia angustifolia Vahl. for short-term storage and germplasm exchange. Acta Physiol Plant 36:635–640

    Article  CAS  Google Scholar 

  • Patil VM (1998) Micropropagation of Ceropegia spp. In Vitro Cell Dev Biol Plant 34:240–243

    Article  Google Scholar 

  • Philip VJ, Nainar SAZ (1986) Clonal Propagation of Vanilla planifolia (Salisb.) Ames using Tissue Culture. J Plant Physio 122(3):211–215

    Article  Google Scholar 

  • Phulwaria M, Shekhawat NS, Rathore JS, Singh RP (2013) An efficient in vitro regeneration and ex vitro rooting of Ceropegia bulbosa Roxb. – a threatened and pharmaceutical important plant of Indian Thar Desert. Ind Crops Prods 42:25–29

    Article  CAS  Google Scholar 

  • Pignatti S (1982) Flora d’Italia, vol 3. Edagricole, Bologna

    Google Scholar 

  • PLANTS Database (2015) Vanda coerulea. Natural Resources Conservation Service USDA

    Google Scholar 

  • Pradhan UC (1985) Red data sheet on Indian Orchidaceae – I Yanda coerulea Griff. ex Lindl. Ind Orchid J 1:54

    Google Scholar 

  • Pradhan UC, Lachungpa ML (1990) Sikkim Himalayan Rhododendrons. Primulaceae Books, Kalimpong

    Google Scholar 

  • Pradhan S, Regmi T, Parmar G, Pant B (2013) Effect of different media on in vitro seed germination and seedling development of Cymbidium aloifolium (L.) Sw. Nepal J Sci Technol 14:51–56

    Article  Google Scholar 

  • Pradhan S, Tiruwa B, Subedee BR, Pant B (2014) In vitro germination and propagation of a threatened medicinal orchid, Cymbidium aloifolium (L.) Sw. through artificial seed. Asian Pac J Trop Biomed 4:971–976

    Article  CAS  Google Scholar 

  • Pradhan S, Tiruwa BL, Subedee BR, Pant B (2016) Efficient plant regeneration of Cymbidium aloifolium (L.) Sw., a threatened orchid of Nepal through artificial seed technology. Am J Plant Sci 7:1964–1974

    Article  Google Scholar 

  • Purohit SD, Dave A (1996) Micropropagation of Sterculia urens Roxb. – an endangered tree species. Plant Cell Rep 15:704–706

    Article  CAS  PubMed  Google Scholar 

  • Purushotham PM, Madam AT, Duraisamy P, Senthil K (2015) In vitro propagation and enhancement of phytoconstituents in Withania coagulans, a rare medicinal plant. Bull Env Pharmacol Life Sci 47:122–131

    Google Scholar 

  • Randhawa GS, Mukhopadhyay A (1986) Floriculture in India. Allied, Mumbai

    Google Scholar 

  • Rao TA (1998) Conservation of wild orchids of Kodagu in the Western Ghats. World Wide Fund for Nature, New Delhi, pp 140–143

    Google Scholar 

  • Rao NK (2004) Plant genetic resources: advancing conservation and use through biotechnology. Afr J Biotechnol 3:136–145

    Google Scholar 

  • Raskoti BB (2009) The orchids of Nepal. Bhakta Bahadur Raskoti and Rita Ale, Nepal

    Google Scholar 

  • Rathore MS, Kheni J (2015) Alginate encapsulation and in vitro plantlet regeneration in critically endangered medicinal plant, Withania coagulans (Stocks) Dunal. Proc Natl Acad Sci, India Sect B: Biol Sci 87:129–134

    Article  CAS  Google Scholar 

  • Rathore TS, Singh RP, Shekhawat NS (1991) Clonal propagation of desert teak (Tecomella undulata) through tissue culture. Plant Sci 79:217–222

    Article  CAS  Google Scholar 

  • Rathore MS, Gehlot HS, Shekhawat NS (2010) Biotechnological approaches for propagation and prospecting of important medicinal plants from Indian Thar Desert. Int J Plant Prod 4:67–72

    CAS  Google Scholar 

  • Rathore MS, Shekhawat S, Kaur G, Singh RP, Shekhawat NS (2012) Micropropagation of vegetable rennet (Withania coagulans (Stock) Dunal) – a critically endangered medicinal plant. J Sustain Forest 31:727–746

    Article  Google Scholar 

  • Rathore MS, Mastan SG, Bhatt P, YVD NSCSJ (2016) Shoot regeneration from leaf explants of Withania coagulans (Stocks) Dunal and genetic stability evaluation of regenerates with RAPD and ISSR markers. S Afr J Bot 102:12–17

    Article  CAS  Google Scholar 

  • Reddy CS, Reddy KN, Pattanaik C, Raju VS (2006) Ethnobotanical observations on some endemic plants of Eastern Ghats, India. Ethnobot Leaflets 10:82–91

    Google Scholar 

  • Reinoso J, Rodrıguez-Oubina J, Viera MC (2002) Lista Roja de los Brio’ fitos de Galicia (NO de Espan a). Nova Acta Cient Compostelana (Biol) 12:83–93

    Google Scholar 

  • Rossi W (2002) Orchidee d’Italia. Ministero dell’ambiente e della tutela del territorio, Direzione conservazione della natura: Istituto nazionale per la fauna selvatica, Bologna

    Google Scholar 

  • Roy B, Mandal AB (2008) Development of synthetic seed involving androgenic and pro-embryos in elite indica rice. Indian J Biotechnol 7:515–519

    CAS  Google Scholar 

  • Roy AR, Patel RS, Patel VV, Sajeev S, Deka BC (2011) Asymbiotic seed germination, mass propagation and seedling development of Vanda coerulea Griff ex. Lindl. (Blue Vanda): An in vitro protocol for an endangered orchid. Sci Hortic 128:325–331

    Article  CAS  Google Scholar 

  • Sahijram L, Bahadur B (2015) Somatic embryogenesis. In: Bahadur B, Rajam MV, Sahijram L, Krishnamurthy KV (eds) Plant biology and biotechnology. Springer, New Delhi, pp 315–327

    Chapter  Google Scholar 

  • Sana H, Rani AS (2017) Influence of seasonal and substrate variation on seed germination of Spilanthes acmella Murr. Int J Appl Agri Res 12:333–338

    Google Scholar 

  • SANBI (2017) Statistics: red list of South African plants version 2017.1. http://redlist.sanbi.org/species.php?species=3812-21

  • Sarasan V, Cripps R, Ramsay MM, Atherton C, McMichen M, Predergast G, Rowntreee JK (2006) Conservation in vitro of threatened plants—progress in the past decade. In vitro Cell Dev Biol Plant 42:206–214

    Article  Google Scholar 

  • Saritha KV, Prakash E, Ramamurthy N, Naidu CV (2002) Micropropagation of Spilanthes acmella Murr. Biol Plant 45:581–584

    Article  CAS  Google Scholar 

  • Sarmah DK, Borthakur M, Borua PK (2010) Artificial seed production from encapsulated PLBs regenerated from leaf base of Vanda coerulea Grifft. ex. Lindl.—an endangered orchid. Curr Sci 98:686–690

    CAS  Google Scholar 

  • Sarmast MK, Niazi A, Salehi H, Abolimoghadam A (2015) Silver nanoparticles affect ACS expression in Tecomella undulata in vitro culture. Plant Cell Tissue Organ Cult 121:227–236

    Article  CAS  Google Scholar 

  • Sebastian DP, Benjamin S, Hariharan M (2002) Micropropagation of Rotula aquatica Lour. – an important woody medicinal plant, vol 52, pp 137–144

    Google Scholar 

  • Seeni S, Latha PG (2000) In vitro multiplication and ecorehabilitation of the endangered Blue Vanda. Plant Cell Tissue Organ Cult 61:1–8

    Article  CAS  Google Scholar 

  • Sergio C, Casas C, Brugues M, Cros RM (1994) Red list of bryophytes of the Iberian Peninsula. ICN, Lisboa, pp 1–50

    Google Scholar 

  • Shaheen A, Shahzad A (2015) Nutrient encapsulation of nodal segments of an endangered white cedar for studies of regrowth, short term conservation and ethylene inhibitors influenced ex vitro rooting. Ind Crop Prod 69:204–211

    Article  CAS  Google Scholar 

  • Shailja KK, Soni M, Singh A (2017) In vitro propagation and conservation of an endangered high value medicinal herb Swertia chirayita of temperate Himalayas. Ind J Plant Phys 22:247–257

    Article  CAS  Google Scholar 

  • Sharma N, Chandel KPS (1992) Effect of ascorbic acid on axillary shoot induction in Tylophora indica (Burm. f.) Merrill. Plant Cell Tissue Organ Cult 29:109–113

    Article  CAS  Google Scholar 

  • Sharma S, Shahzad A (2013) Efficient micropropagation of Spilanthes acmella (L.) Murr.: a threatened medicinal herb. Br Biotechnol J 3:405–415

    Article  Google Scholar 

  • Sharma S, Shahzad A (2014) Synseed production in Spilanthes mauritiana DC. for short-term storage and germplasm exchange. Br Biotechnol J 4:696–707

    Article  Google Scholar 

  • Sharma S, Shahzad A, Jan N, Sahai A (2009a) In vitro studies on shoot regeneration through various explants and alignate-encapsulated nodal segments of Spilanthes mauritiana DC., an endangered medicinal herb. Int J Plant Dev Biol 3:56–61

    Google Scholar 

  • Sharma S, Shahzad A, Sahai A (2009b) Artificial seeds for propagation and preservation of Spilanthes acmella (L.) Murr., a threatened pesticidal plant species. Int J Plant Dev Biol 3:62–65

    Google Scholar 

  • Sharma S, Shahzad A, Teixeira da Silva JA (2013) Synseed technology—a complete synthesis. Biotechnol Adv 31:186–207

    Article  CAS  PubMed  Google Scholar 

  • Shekhawat GS, Batra A, Mathur S (2002) A reliable in vitro protocol for rapid mass propagation of Azadirachta indica Juss. J Plant Biol 29:109–112

    Google Scholar 

  • Singh KK (2008) In vitro plant regeneration of an endangered Sikkim Himalayan Rhododendron (R. maddeni Hook. F.) from alignate-encapsulated shoot tips. Biotechnology 7:144–148

    Article  CAS  Google Scholar 

  • Singh D, Gupta RS (2011) Hepatoprotective activity of methanol extract of Tecomella undulata against alcohol and paracetamol induced hepatotoxicity in rats. Life Sci Med Res 26:1–8

    Google Scholar 

  • Singh KK, Gurung B (2009) In vitro propagation of R. maddeni Hook. F. an endangered Rhododendron species of Sikkim Himalaya. Not Bot Hortic Agrobot Cluj 37:79–83

    CAS  Google Scholar 

  • Singh R, Rathore M, Mishra GP, Kumar M, Singh R, Ahmed Z (2009a) Adventitious shoot regeneration and Agrobacterium tumefaciens mediated transformation in Rohida (Tecomella undulata). Indian Forest 135:751–764

    CAS  Google Scholar 

  • Singh SK, Rai MK, Asthana P, Pandey S, Jaiswal VS, Jaiswal U (2009b) Plant regeneration from alginate-encapsulated shoot tips of Spilanthes acmella (L.) Murr., a medicinally important and herbal pesticidal plant species. Acta Physiol Plant 31:649–653

    Article  CAS  Google Scholar 

  • Singh SK, Rai MK, Asthana P, Sahoo L (2009c) An improved micropropagation of Spilanthes acmella through thin cell layer culture. Acta Physiol Plant 31:693–698

    Article  CAS  Google Scholar 

  • Sinha P, Hakim ML, Alam MF (2009) In vitro mass clonal propagation of Spathoglottis plicata Blume. Plant Tiss Cult Biotechnol 19:151–160

    Article  Google Scholar 

  • Sivarajan VV, Balachandran I (1994) Ayurvedic drugs and their plant sources. Oxford & IBH, New Delhi, pp 195–198

    Google Scholar 

  • Spinoso-Castillo JL, Chavez-Santoscoy RA, Bogdanchikova N, Pérez-Sato JA, Morales-Ramos V, Bello-Bello JJ (2017) Antimicrobial and hormetic effects of silver nanoparticles on in vitro regeneration of vanilla (Vanilla planifolia Jacks. ex Andrews) using a temporary immersion system. Plant Cell Tissue Organ Cult 129:195–207

    Article  CAS  Google Scholar 

  • Stojakowska A (1993) Micropropagation of Urginea maritima (L.) Baker S. STR. Acta Bot Pol 62:11–15

    Article  Google Scholar 

  • Sunnichan VG, Shivanna KR, Mohan Ram HY (1998) Micropropagation of gum karaya (Sterculia urens) by adventitious shoot formation and somatic embryogenesis. Plant Cell Rep 17:951–956

    Article  CAS  PubMed  Google Scholar 

  • Tas IC, Van Dijk PJ (1999) Crosses between sexual and apomictic dandelions (Taraxacum). I. The inheritance of apomixis. Heredity 83:707–714

    Article  PubMed  Google Scholar 

  • Thorpe TA, Harry IS, Kumar PP (1991) Application of micropropagation to forestry. In: Debergh PC, Zimmerman RH (eds) Micropropagation: technology and application. Kluwer Academic, Dordrecht, pp 311–336

    Chapter  Google Scholar 

  • Tiwari KL, Jadhav SK, Joshi V (2011) An updated review on medicinal herb genus Spilanthes. Chin J Integr Med 9:1170–1178

    Article  CAS  Google Scholar 

  • Trejgell A, Chernetskyy M, Podlasiak J, Tretyn A (2013) An efficient system for regenerating Taraxacum pieninicum Pawł. from seedling explants. Acta Biol Cracov Ser Bot 55:73–79

    Google Scholar 

  • Trunjaruen A, Taratima W (2018) An effective micropropagation of Cymbidium aloifolium (L.) Sw. Thai J Bot 10:77–91

    Google Scholar 

  • Tyagi H, Tomar UK (2013) Factors Affecting in vitro shoot proliferation and rooting of mature Tecomella undulata (Sm) seem tree. Res Plant Sci 1:38–44

    Google Scholar 

  • Varshney A, Anis M (2012) Improvement of shoot morphogenesis in vitro and assessment of changes of the activity of antioxidant enzymes during acclimation of micropropagated plants of Desert Teak. Acta Physiol Plant 34:859–867

    Article  CAS  Google Scholar 

  • Vasudevan R, Van Staden J (2010) In vitro asymbiotic seed germination and seedling growth of Ansellia africana Lindl. Sci Hortic 123:496–504

    Article  CAS  Google Scholar 

  • Vasudevan R, Van Staden J (2011) Cytokinin and explant types influence in vitro plant regeneration of Leopard Orchid (Ansellia africana Lindl.). Plant Cell Tissue Organ Cult 107:123

    Article  CAS  Google Scholar 

  • Wang L, Lizhe A, Hu Y, Lixin W, Yi L (2009) Influence of phytohormones and medium on the shoot regeneration from the leaf of S. chirayita Buch.–Ham. ex Wall. in vitro. Afr J Biotechnol 8:2513–2517

    CAS  Google Scholar 

  • Watt JM, Brayer-Brandwijk MG (1962) The medicinal and poisonous plants of Southern and Eastern Africa, 2nd edn. E&S Livingstone, Edinburgh

    Google Scholar 

  • Wawrosch C, Maskay N, Kopp B (1999) Micropropagation of the threatened Nepalese medicinal plant Swertia chirata Buch.–Ham. ex Wall. Plant Cell Rep 18:997–1001

    Article  CAS  Google Scholar 

  • Wesley PS, Devi BC, Shibu BS, Moin S (2013) In vitro propagation of Coelogyne breviscapa Lindl., Dendrobium aqueum Lindl., and Flickingeria nodosa (Dalz.) Seidenf. via asymbiotic seed germination. Asian Pac J Mol Biol Biotechnol 2:26–32

    Google Scholar 

  • Williams VL, Raimondo D, Crouch NR, Brueton VJ, Cunningham AB, Scott-Shaw CR, Lötter M, Ngwenya AM (2016) Drimia altissima (L.f.) Ker Gawl. National Assessment: Red List of South African Plants version 2017.1. http://redlist.sanbi.org/species.php?species=3812-21

  • Wongsawatkul O, Prachayasittikul S, Isarankura-Na-Ayudhya C, Satayavivad J, Ruchirawat S, Prachayasittikul V (2008) Vasorelaxant and antioxidant activities of Spilanthes acmella Murr. Int J Mol Sci 9:2724–2744

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yadav K, Singh N, Aggarwal A (2012) Arbuscular mycorrhizal technology for the growth enhancement of micropropagated Spilanthes acmella Murr. Plant Protect Sci 48:31–36

    Article  CAS  Google Scholar 

  • Yang L, Qin LH, Bligh SW (2006) A new phenanthrene with a spirolactone from Dendrobium chrysanthum and its anti-inflammatory activities. Bioorg Med Chem 14:3496–3501

    Article  CAS  PubMed  Google Scholar 

  • Ye Q, Qin G, Zhao W (2002) Immunomodulatory sesquiterpene glycosides from Dendrobium nobile. Phytochemistry 61:885–890

    Article  CAS  PubMed  Google Scholar 

  • Zakhlenjuk OV, Kunakh VA (1998) Arnebia euchroma: in vitro culture and the production of shikonin and other secondary metabolites. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, Medicinal and aromatic plants X, vol 41. Springer, New York, pp 28–44

    Google Scholar 

  • Zobolo AM (2010) Effect of temperature, light intensity and growth regulators on propagation of Ansellia Africana from cuttings. Afr J Biotechnol 9:5566–5574

    CAS  Google Scholar 

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Authors and Affiliations

  1. Plant Cell Biotechnology Department, CSIR-CFTRI, Mysuru, Karnataka, India

    B. Nandini & P. Giridhar

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  1. B. Nandini
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  2. P. Giridhar
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  1. Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

    Mohammad Faisal

  2. Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

    Abdulrahman A. Alatar

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Nandini, B., Giridhar, P. (2019). Insight View of Topical Trends on Synthetic Seeds of Rare and Endangered Plant Species and Its Future Prospects. In: Faisal, M., Alatar, A. (eds) Synthetic Seeds . Springer, Cham. https://doi.org/10.1007/978-3-030-24631-0_5

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