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Synthetic Seeds pp 21–60Cite as

Synthetic Seeds: Relevance to Endangered Germplasm Conservation In Vitro

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Abstract

The twentieth century witnessed deterioration of biodiversity and loss of natural habitats of many plant species. However, it was also an era of significant progress in tissue culture technology which opened further vistas for multiplication and conservation of plant species. Synthetic seed technology is one such method which involves selection of a suitable explant and encapsulating it in an apposite matrix for successful germination and conversion into a healthy plantlet. The underlying basis of synthetic seed technology is to imitate natural plant development that occurs through seed germination. This method has been successfully employed for propagation and storage of various forest, medicinal, and vegetable plant species. The technique is of pivotal importance for species which produce non-viable seeds, recalcitrant seeds, or have limited and rare seed production. Threatened and endangered plant species are one such category which has several bottlenecks in seed development, reproduction, and establishment in their natural environments that have undergone disruptive changes. This review aims to explore and assess the potential of synthetic seed technology as an effective approach to support conservation strategies for endangered plant species.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

2iP:

Isopentenyl adenine

AA:

Ascorbic acid

ABA:

Abscisic acid

BAP/BA:

6-Benzyladenine

CR:

Critically endangered

DD:

Data deficient

EC:

Embryogenic cells

ES:

Endangered species

GA3 :

Gibberellic acid

H2SO −4 :

Sulphuric acid

IAA:

Indole-3-acetic acid

KN:

Kinetin

MS:

Murashige and Skoog media

NAA:

α-Naphthaleneacetic acid

PGR:

Plant growth regulators

SE:

Somatic embryo

TDZ:

Thidiazuron

tTCL:

Thin cell layers

References

  • Aberham A, Pieri V, Croom EM Jr, Ellmerer E, Stuppner H (2011) Analysis of iridoids, secoiridoids and xanthones in Centaurium erythraea, Frasera caroliniensis and Gentiana lutea using LC–MS and RP-HPLC. J Pharm Biomed Anal 54(3):517–525

    Article  CAS  PubMed  Google Scholar 

  • Aguado M, Vicente MJ, Miralles J, Franco JA, Martínez-Sánchez JJ (2012) Aerial seed bank and dispersal traits in Anthemis chrysantha (Asteraceae), a critically endangered species. Flora 207(4):275–282

    Article  Google Scholar 

  • Aguraiuja R (2011) Reintroduction of the endangered fern species Woodsia ilvensis to Estonia: a long-term pilot study. Biodivers Conserv 20(2):391–400

    Article  Google Scholar 

  • Ahmed EM (2015) Hydrogel: preparation, characterization, and applications: a review. J Adv Res 6(2):105–121

    Article  CAS  PubMed  Google Scholar 

  • Al-Gendy AA, Bakr RO, El-gindi OD (2013) Somatic embryogenesis and plant regeneration from callus and suspension cultures of Iphiona mucronata (Forssk). Eur Sci J 9(27):37–49

    Google Scholar 

  • Antony JJJ, Sinniah UR, Keng CL, Pobathy R, Khoddamzadeh AA, Subramaniam S (2011) Selected potential encapsulation-dehydration parameters on ‘dendrobium’ bobby messina protocorm-like bodies using ttc analysis. Aust J Crop Sci 5(13):1817

    CAS  Google Scholar 

  • Ayuso M, Ramil-Rego P, Landin M, Gallego PP, Barreal ME (2017) Computer-assisted recovery of threatened plants: keys for breaking seed dormancy of Eryngium viviparum. Front Plant Sci 8:2092

    Article  PubMed  PubMed Central  Google Scholar 

  • Bairu MW, Fennell CW, van Staden J (2006) The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. ‘Zelig’). Sci Hortic 108(4):347–351

    Article  CAS  Google Scholar 

  • Bakhshaie M, Babalar M, Mirmasoumi M, Khalighi A (2010) Somatic embryogenesis and plant regeneration of Lilium ledebourii (Baker) Boiss., an endangered species. Plant Cell Tissue Organ Cult 102(2):229–235

    Article  CAS  Google Scholar 

  • Banerjee S, Singh S, Pandey H, Pandey P, ur Rahman L (2012) Conservation and storage of Curcuma amada Roxb. synthetic seeds on Luffa sponge matrix and RAPD analysis of the converted plantlets. Ind Crop Prod 36(1):383–388

    Article  CAS  Google Scholar 

  • Banu LA, Rashid MHO, Miah MB (2014) Development of artificial seed and preservation in Mimosa pudica L., an important medicinal plant in Bangladesh. J Bio-Sci 22:89–99

    Article  Google Scholar 

  • Bapat VA, Mhatre M, Rao PS (1987) Propagation of Morus indica L. (mulberry) by encapsulated shoot buds. Plant Cell Rep 6(5):393–395

    Article  CAS  PubMed  Google Scholar 

  • Barret P, Brinkman M, Beckert M (2006) A sequence related to rice Pong transposable element displays transcriptional activation by in vitro culture and reveals somaclonal variations in maize. Genome 49(11):1399–1407

    Article  CAS  PubMed  Google Scholar 

  • Baskin JM (1997) Methods of breaking seed dormancy in the endangered species Iliamna corei (Sherff) Sherff (Malvaceae), with special attention to heating. Nat Areas J 17:313–323

    Google Scholar 

  • Baskin CC, Baskin JM (1998) Seeds: ecology, biogeography, and evolution of dormancy and germination. Academic Press, San Diego

    Google Scholar 

  • Baskin JM, Baskin CC (2004) A classification system for seed dormancy. Seed Sci Res 14(1):1–16

    Article  Google Scholar 

  • Baskin JM, Baskin CC, Li X (2000) Taxonomy, anatomy and evolution of physical dormancy in seeds. Plant Species Biol 15(2):139–152

    Article  Google Scholar 

  • Belwal NS, Kamal B, Sharma V, Gupta S, Kumar Dobriyal A, Jadon VS (2016) Production of genetically uniform plants from shoot tips of Aconitum heterophyllum Wall – a critically endangered medicinal herb. J Hortic Sci Biotechnol 91(5):529–535

    Article  CAS  Google Scholar 

  • Benelli C (2016) Encapsulation of shoot tips and nodal segments for in vitro storage of “Kober 5BB” grapevine rootstock. Horticulturae 2(3):10

    Article  Google Scholar 

  • Benson EE, Reed BM, Brennan RM, Clacher KA, Ross DA (1996) Use of thermal analysis in the evaluation of cryopreservation protocols for Ribes nigrum L. germplasm. CryoLetters (UK) 17:347–362

    Google Scholar 

  • Bentsink L, Koornneef M (2008) Seed dormancy and germination. In: Somerville CR, Meyerowitz EM (eds) The Arabidopsis book, vol 6. American Society of Plant Biologists, Rockville

    Article  PubMed  PubMed Central  Google Scholar 

  • Bilz M, Kell SP, Maxted N, Lansdown RV (2011) European red list of vascular plants. European Union, Luxembourg

    Google Scholar 

  • Bordallo PN, Silva DH, Maria J, Cruz CD, Fontes EP (2004) Somaclonal variation on in vitro callus culture potato cultivars. Hortic Bras 22(2):300–304

    Article  Google Scholar 

  • Bradbeer JW (1988) The breaking of seed dormancy. In: Seed dormancy and germination. Springer, Boston, pp 55–79

    Chapter  Google Scholar 

  • Brischia R, Piccioni E, Standardi A (2002) Micropropagation and synthetic seed in M. 26 apple rootstock (II): a new protocol for production of encapsulated differentiating propagules. Plant Cell Tissue Organ Cult 68(2):137–141

    Article  Google Scholar 

  • Canale GR, Suscke P, Rocha-Santos L, São Bernardo CS, Kierulff MCM, Chivers DJ (2016) Seed dispersal of threatened tree species by a critically endangered primate in a Brazilian Hotspot. Folia Primatol 87(3):123–140

    Article  Google Scholar 

  • Carqué-Álamo E, Marrero-Gómez M, Banares-Baudet A (2003) Seed germination and dormancy in Echium acanthocarpum Svent.(Boraginaceae), an endangered plant of the Canary Islands, Spain. Nat Areas J 23(4):362–367

    Google Scholar 

  • Cenkci S, Kargioglu M, Dayan S, Konuk M (2008) In vitro propagation of an endangered plant species, Thermopsis turcica (Fabaceae). Biologia 63(5):652–657

    Article  Google Scholar 

  • Chand S, Singh AK (2004) Plant regeneration from encapsulated nodal segments of Dalbergia sissoo Roxb., a timber-yielding leguminous tree species. J Plant Physiol 161(2):237

    Article  CAS  PubMed  Google Scholar 

  • Chanyenga TF, Geldenhuys CJ, Sileshi GW (2012) Germination response and viability of an endangered tropical conifer Widdringtonia whytei seeds to temperature and light. S Afr J Bot 81:25–28

    Article  Google Scholar 

  • Chavan JJ, Nalawade AS, Gaikwad NB, Gurav RV, Dixit GB, Yadav SR (2014) An efficient in vitro regeneration of Ceropegia noorjahaniae: an endemic and critically endangered medicinal herb of the Western Ghats. Physiol Mol Biol Plants 20(3):405–410

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Chavan JJ, Gaikwad NB, Kshirsagar PR, Umdale SD, Bhat KV, Dixit GB, Yadav SR (2015) Highly efficient in vitro proliferation and genetic stability analysis of micropropagated Ceropegia evansii by RAPD and ISSR markers: a critically endangered plant of Western Ghats. Plant Biosyst 149(2):442–450

    Article  Google Scholar 

  • Chavez VM, Litz RE, Moon PA, Norstog K (1992) Somatic embryogenesis from leaf callus of mature plants of the gymnosperm Ceratozamia mexicana var. robusta (Miq.) dyer (Cycadales). In Vitro Plant 28(2):59–63

    Article  Google Scholar 

  • Chesser JM, Brewer JS (2011) Factors influencing seedling recruitment in a critically endangered pitcher plant, Sarracenia rubra ssp. alabamensis. Endanger Species Res 13(3):245–252

    Article  Google Scholar 

  • Choi YE, Kim JW, Yoon ES (1999) High frequency of plant production via somatic embryogenesis from callus or cell suspension cultures in Eleutherococcus senticosus. Ann Bot 83(3):309–314

    Article  CAS  Google Scholar 

  • Chuang SJ, Chen CL, Chen JJ, Chou WY, Sung JM (2009) Detection of somaclonal variation in micro-propagated Echinacea purpurea using AFLP marker. Sci Hortic 120(1):121–126

    Article  CAS  Google Scholar 

  • Copeland LO, McDonald MB (2001) Seed germination. In: Principles of seed science and technology. Springer, Boston, pp 72–123

    Chapter  Google Scholar 

  • Copete E, Herranz JM, Ferrandis P, Baskin CC, Baskin JM (2011) Physiology, morphology and phenology of seed dormancy break and germination in the endemic Iberian species Narcissus hispanicus (Amaryllidaceae). Ann Bot 107(6):1003–1016

    Article  PubMed  PubMed Central  Google Scholar 

  • Cuena-Lombraña A, Porceddu M, Dettori CA, Bacchetta G (2017) Discovering the type of seed dormancy and temperature requirements for seed germination of Gentiana lutea L. subsp. lutea (Gentianaceae). J Plant Ecol 11(2):308–316

    Article  Google Scholar 

  • Da Silva DPC, Paiva R, Herrera RC, Silva LC, Ferreira GN, dos Reis MV (2018) Somatic embryogenesis of Byrsonima intermedia A. Juss.: induction and maturation via indirect approach. Plant Cell Tissue Organ Cult 133(1):115–122

    Article  CAS  Google Scholar 

  • Dang JC, Kumaria S, Kumar S, Tandon P (2011) Micropropagation of Ilex khasiana, a critically endangered and endemic holly of Northeast India. AoB Plants 2011:plr012

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Daud N, Taha RM, Hasbullah NA (2008) Artificial seed production from encapsulated micro shoots of Saintpaulia ionantha Wendl. (African Violet). J Appl Sci 8(24):4662–4667

    Article  Google Scholar 

  • De Vitis M, Seal CE, Ulian T, Pritchard HW, Magrini S, Fabrini G, Mattana E (2014) Rapid adaptation of seed germination requirements of the threatened Mediterranean species Malcolmia littorea (Brassicaceae) and implications for its reintroduction. S Afr J Bot 94:46–50

    Article  Google Scholar 

  • Dey A, De JN (2011) Ethnobotanical aspects of Rauvolfia serpentina (L). Benth. ex Kurz. in India, Nepal and Bangladesh. J Med Plants Res 5(2):144–150

    Google Scholar 

  • Dey T, Saha S, Ghosh PD (2015) Somaclonal variation among somatic embryo derived plants—evaluation of agronomically important somaclones and detection of genetic changes by RAPD in Cymbopogon winterianus. S Afr J Bot 96:112–121

    Article  Google Scholar 

  • Ebrahimi M, Mokhtari A, Amirian R (2018) A highly efficient method for somatic embryogenesis of Kelussia odoratissima Mozaff., an endangered medicinal plant. Plant Cell Tissue Organ Cult 132(1):99–110

    Article  CAS  Google Scholar 

  • Engelmann F (2000) Importance of cryopreservation for the conservation of plant genetic resources. In: Cryopreservation of tropical plant germplasm: current research progress and application. Proceedings of an international workshop, Tsukuba, Japan, October, 1998. International Plant Genetic Resources Institute (IPGRI), pp 8–20

    Google Scholar 

  • Engelmann F (2004) Plant cryopreservation: progress and prospects. In Vitro Cell Dev Biol Plant 40(5):427–433

    Article  Google Scholar 

  • Etienne H, Bertrand B (2003) Somaclonal variation in Coffea arabica: effects of genotype and embryogenic cell suspension age on frequency and phenotype of variants. Tree Physiol 23(6):419–426

    Article  CAS  PubMed  Google Scholar 

  • Fabre J, Dereuddre J (1990) Encapsulation-dehydration: a new approach to cryopreservation of Solanum shoot-tips. CryoLetters 11:413–426

    Google Scholar 

  • Faisal M, Anis M (2007) Regeneration of plants from alginate-encapsulated shoots of Tylophora indica (Burm. f.) Merrill, an endangered medicinal plant. J Hortic Sci Biotechnol 82(3):351–354

    Article  CAS  Google Scholar 

  • Faisal M, Shahzad A, Anis M (2008) Somatic embryogenesis and plant regeneration from nodal explants in Psoralea corylifolia L. Int J Plant Dev Biol 2(2):111–113

    Google Scholar 

  • Faisal M, Alatar AA, Hegazy AK (2013) Molecular and biochemical characterization in Rauvolfia tetraphylla plantlets grown from synthetic seeds following in vitro cold storage. Appl Biochem Biotechnol 169(2):408–417

    Article  CAS  PubMed  Google Scholar 

  • Farjon, A. (2013). Widdringtonia whytei. The IUCN Red List of Threatened Species

    Google Scholar 

  • Finch-Savage WE, Footitt S (2017) Seed dormancy cycling and the regulation of dormancy mechanisms to time germination in variable field environments. J Exp Bot 68(4):843–856

    Article  CAS  PubMed  Google Scholar 

  • Flachsland E, Terada G, Scocchi A, Rey H, Mroginski L, Engelmann F (2006) Cryopreservation of seeds and in vitro-cultured protocorms of Oncidium bifolium Sims. (Orchidaceae) by encapsulation-dehydration. CryoLetters 27(4):235–242

    CAS  PubMed  Google Scholar 

  • Frankel OH (1970) Variation-the essence of life. Proc Linn Soc NSW 95(2):158–169

    Google Scholar 

  • Frankel O, Soulé ME (1981) Conservation and evolution. Cambridge University Press, Cambridge

    Google Scholar 

  • Fujii JAA, Slade D, Aguirre-Rascon J, Redenbaugh K (1992) Field planting of alfalfa artificial seeds. In Vitro Plant 28(2):73–80

    Article  Google Scholar 

  • Gantait S, Sinniah UR (2013) Storability, post-storage conversion and genetic stability assessment of alginate-encapsulated shoot tips of monopodial orchid hybrid Aranda Wan Chark Kuan ‘Blue’× Vanda coerulea Grifft. ex. Lindl. Plant Biotechnol Rep 7(3):257–266

    Article  Google Scholar 

  • Gantait S, Kundu S (2017) Artificial seed technology for storage and exchange of plant genetic resources. In: Advanced technologies for crop improvement and agricultural productivity. Agrobios (International), Jodhpur, pp 135–159

    Google Scholar 

  • Gantait S, Kundu S, Ali N, Sahu NC (2015) Synthetic seed production of medicinal plants: a review on influence of explants, encapsulation agent and matrix. Acta Physiol Plant 37(5):98

    Article  CAS  Google Scholar 

  • Gantait S, Vijayan J, Majee A (2017) Artificial seed production of Tylophora indica for interim storing and swapping of germplasm. Hortic Plant J 3(1):41–46

    Article  Google Scholar 

  • Gao DY, Vallejo VA, He B, Gai YC, Sun LH (2009) Detection of DNA changes in somaclonal mutants of rice using SSR markers and transposon display. Plant Cell Tissue Organ Cult 98(2):187–196

    Article  CAS  Google Scholar 

  • Geneve RL, Kester ST (2018) Morphophysiological seed dormancy in Heptacodium. Seed Sci Res 28(3):192–196

    Article  Google Scholar 

  • George EF (1993) Plant propagation by tissue culture. Part 1: the technology. Exegetics, London

    Google Scholar 

  • Giménez C, De Garcia E, De Enrech NX, Blanca I (2001) Somaclonal variation in banana: cytogenetic and molecular characterization of the somaclonal variant CIEN BTA-03. In Vitro Cell Dev Biol Plant 37(2):217–222

    Article  Google Scholar 

  • Gnasekaran P, Mahmood M, Subramaniam S (2016) Ultrastructure study of Vanda Kasem’s Delight orchid’s protocorm-like body. Hortic Bras 34(3):333–339

    Article  Google Scholar 

  • Godefroid S, Van de Vyver A, Vanderborght T (2010) Germination capacity and viability of threatened species collections in seed banks. Biodivers Conserv 19(5):1365–1383

    Article  Google Scholar 

  • Gómez-González S, Paniw M, Antunes K, Ojeda F (2018) Heat shock and plant leachates regulate seed germination of the endangered carnivorous plant Drosophyllum lusitanicum. Web Ecol 18(1):7–13

    Article  Google Scholar 

  • Gonçalves S, Martins N, Romano A (2009) Micropropagation and conservation of endangered species Plantago algarbiensis and P. almogravensis. Biol Plant 53(4):774

    Article  Google Scholar 

  • Gonçalves S, Fernandes L, Romano A (2010) High-frequency in vitro propagation of the endangered species Tuberaria major. Plant Cell Tissue Organ Cult 101(3):359–363

    Article  Google Scholar 

  • Gonza ME, Pe C, Viviani AB (1997) Cryopreservation of nodal explants of an endangered plant species (Centaurium rigualii Esteve) using the encapsulation–dehydration method. Biodivers Conserv 6(4):583–590

    Article  Google Scholar 

  • González-López Ó, Casquero PA (2014) Effects of GA3 pregerminative treatment on Gentiana lutea L. var. aurantiaca germination and seedlings morphology. Sci World J 2014:751279

    Article  CAS  Google Scholar 

  • Gupta PK, Kreitinger M (1993) Synthetic seeds in forest trees. In: Micropropagation of woody plants. Springer, Dordrecht, pp 107–119

    Chapter  Google Scholar 

  • Halim NAA, Tan BC, Midin MR, Madon M, Khalid N, Yaacob JS (2018) Abscisic acid and salinity stress induced somaclonal variation and increased histone deacetylase (HDAC) activity in Ananas comosus var. MD2. Plant Cell Tissue Organ Cult 133(1):123–135

    Article  CAS  Google Scholar 

  • Hansen DM, Müller CB (2009) Invasive ants disrupt gecko pollination and seed dispersal of the endangered plant Roussea simplex in Mauritius. Biotropica 41(2):202–208

    Article  Google Scholar 

  • Harvey-Brown Y (2018). Aquilaria malaccensis. IUCN Red List Threatened Species 2018:e.T32056A2810130. https://doi.org/10.2305/IUCN.UK.2018-1.RLTS.T32056A2810130.en

  • Hazubska-Przybył T, Wawrzyniak M, Obarska A, Bojarczuk K (2015) Effect of partial drying and desiccation on somatic seedling quality in Norway and Serbian spruce. Acta Physiol Plant 37(1):1735

    Article  Google Scholar 

  • Hegde V, Makeshkumar T, Sheela MN, Chandra CV, Koundinya AVV, Anil SR, Muthuraj R, Darshan S (2017) Production of synthetic seed in Cassava (Manihot esculenta Crantz). J Root Crops 42(2):5–9

    Google Scholar 

  • Holobiuc I, Catana R (2012) Recurrent somatic embryogenesis in long-term cultures of Gentiana lutea L. as a source for synthetic seed production for medium-term preservation. Arch Biol Sci 64(2):809–817

    Article  Google Scholar 

  • Hosseini SM (2014) Efficiency study of Ellenberg method application for ecological evaluation of herbal sites (Case study: ecological evaluation of Lilium ledebourii site in Gilan Province). JBES 4:168–173

    Google Scholar 

  • Howe HF, Smallwood J (1982) Ecology of seed dispersal. Annu Rev Ecol Syst 13(1):201–228

    Article  Google Scholar 

  • Hung CD, Dung CD (2015) Production of chrysanthemum synthetic seeds under non-aseptic conditions for direct transfer to commercial greenhouses. Plant Cell Tissue Organ Cult 122(3):639–648

    Article  Google Scholar 

  • Ikeda H, Itoh K (2001) Germination and water dispersal of seeds from a threatened plant species Penthorum chinense. Ecol Res 16(1):99–106

    Article  Google Scholar 

  • Imanishi A, Imanishi J (2014) Seed dormancy and germination traits of an endangered aquatic plant species, Euryale ferox Salisb. (Nymphaeaceae). Aquat Bot 119:80–83

    Article  Google Scholar 

  • Inpuay K, Te-chato S (2012) Primary and secondary somatic embryos as tool for the propagation and artificial seed production of oil palm. J Agric Technol 8(2):597–609

    Google Scholar 

  • Isabel N, Boivin R, Levasseur C, Charest PM, Bousquet J, Tremblay FM (1996) Occurrence of somaclonal variation among somatic embryo-derived white spruces (Picea glauca, Pinaceae). Am J Bot 83(9):1121–1130

    Article  Google Scholar 

  • Islam MS, Bari MA (2012) In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L. J Bio-Sci 20:99–108

    Article  Google Scholar 

  • Israeli Y, Ben-Bassat D, Reuveni O (1996) Selection of stable banana clones which do not produce dwarf somaclonal variants during in vitro culture. Sci Hortic 67(3–4):197–205

    Article  Google Scholar 

  • IUCN Standards and Petitions Subcommittee (2017) Guidelines for using the IUCN Red list Categories and Criteria. Version 13. Prepared by the Standards and Petitions Subcommittee. http://www.iucnredlist.org/documents/RedListGuidelines.pdf

  • Jacobsen JV, Pressman E (1979) A structural study of germination in celery (Apium graveolens L.) seed with emphasis on endosperm breakdown. Planta 144(3):241–248

    Article  CAS  PubMed  Google Scholar 

  • Jäger AK, van Staden J (1996) Somatic embryogenesis in Encephalartos cycadifolius. Plant Cell Rep 15(6):437–440

    Article  PubMed  Google Scholar 

  • Javed SB, Alatar AA, Anis M, Faisal M (2017) Synthetic seeds production and germination studies, for short term storage and long distance transport of Erythrina variegata L.: a multipurpose tree legume. Ind Crop Prod 105:41–46

    Article  CAS  Google Scholar 

  • Jayasankar S, Van Aman M, Cordts J, Dhekney S, Li ZT, Gray DJ (2005) Low temperature storage of suspension culture-derived grapevine somatic embryos and regeneration of plants. In Vitro Cell Dev Biol Plant 41(6):752

    Article  Google Scholar 

  • Jitsopakul N, Thammasiri K, Ishikawa K (2008) Cryopreservation of Vanda coerulea protocorms by encapsulation-dehydration. CryoLetters 29(3):253–260

    PubMed  Google Scholar 

  • Joshi P, Dhawan V (2005) Swertia chirayita – an overview. Curr Sci 89:635–640

    CAS  Google Scholar 

  • Joshi P, Dhawan V (2007) Axillary multiplication of Swertia chirayita (Roxb. Ex Fleming) H. Karst., a critically endangered medicinal herb of temperate Himalayas. In Vitro Cell Dev Biol Plant 43(6):631–638

    Article  CAS  Google Scholar 

  • Joshi RK, Rao GJN (2009) Somaclonal variation in submergence tolerant rice cultivars and induced diversity evaluation by PCR markers. Int J Genet Mol Biol 1(5):080–088

    CAS  Google Scholar 

  • Joy PP, Thomas J, Mathew S, Skaria BP (2004) Curculigo orchioideds: a plant for health care. Indian J Arecanut Spices Med Plants 6:131–134

    Google Scholar 

  • Kang KS, Veeder GT, Mirrasoul PJ, Kaneko T, Cottrell IW (1982) Agar-like polysaccharide produced by a Pseudomonas species: production and basic properties. Appl Environ Microbiol 43(5):1086–1091

    CAS  PubMed  PubMed Central  Google Scholar 

  • Kang HD, Moon HK, Lee SK (2014) Micropropagation via somatic embryogenesis of rare and endangered species, Acanthopanax seoulenses Nakai. For Sci Technol 10(4):190–196

    Google Scholar 

  • Kant T, Prajapati S, Parmar AK (2010) Efficient micropropagation from cotyledonary node cultures of Commiphora wightii (Arn.) Bhandari, an endangered medicinally important desert plant. J Plant Dev 17:37–48

    Google Scholar 

  • Karam NS, Al-Majathoub M (2000) Direct shoot regeneration and microtuberization in wild Cyclamen persicum Mill. using seedling tissue. Sci Hortic 86(3):235–246

    Article  CAS  Google Scholar 

  • Kato R, Kadono Y (2011) Seed germination traits of Trapella sinensis (Trapellaceae), an endangered aquatic plant in Japan: conservation implications. Aquat Bot 95(4):258–261

    Article  Google Scholar 

  • Kaulfuß F, Reisch C (2017) Reintroduction of the endangered and endemic plant species Cochlearia bavarica—implications from conservation genetics. Ecol Evol 7(24):11100–11112

    Article  PubMed  PubMed Central  Google Scholar 

  • Kaur H, Singh K (2012) A brief phytopharmacological overview of Tylophora indica – an endangered medicinal plant. Int J Pharm Sci Res 3(11):4073

    Google Scholar 

  • Kawiak A, Łojkowska E (2004) Application of RAPD in the determination of genetic fidelity in micropropagated Drosera plantlets. In Vitro Cell Dev Biol Plant 40(6):592–595

    Article  CAS  Google Scholar 

  • Kharkwal AC, Kushwaha R, Prakash O, Ogra RK, Bhattacharya A, Nagar PK, Ahuja PS (2008) An efficient method of propagation of Podophyllum hexandrum: an endangered medicinal plant of the Western Himalayas under ex situ conditions. J Nat Med 62(2):211–216

    Article  CAS  PubMed  Google Scholar 

  • Khilwani B, Kaur A, Ranjan R, Kumar A (2016) Direct somatic embryogenesis and encapsulation of somatic embryos for in vitro conservation of Bacopa monnieri (L.) Wettst. Plant Cell, Tissue and Organ Culture (PCTOC) 127(2):433–442

    Article  CAS  Google Scholar 

  • Khoddamzadeh AA, Sinniah UR, Lynch P, Kadir MA, Kadzimin SB, Mahmood M (2011) Cryopreservation of protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb. f.) Christenson by encapsulation-dehydration. Plant Cell Tissue Organ Cult 107(3):471–481

    Article  Google Scholar 

  • Khrolenko YA, Burundukova OL (2013) Ecological features of the leaf structure and plastid apparatus of Far East Araliaceae species. Contemp Probl Ecol 6(4):374–380

    Article  Google Scholar 

  • Kim MA, Park JK (2002) High frequency plant regeneration of garlic (Allium sativum L.) calli immobilized in calcium alginate gel. Biotechnol Bioprocess Eng 7(4):206

    Article  CAS  Google Scholar 

  • Kitto SL, Janick J (1985) Production of synthetic seeds by encapsulating asexual embryos of carrot. J Am Soc Hortic Sci 110:277–282

    Google Scholar 

  • Krishnamurthi M, Tlaskal J (1974) Fiji disease resistant Saccharum officinarum var. Pindar subclones from tissue cultures. Proc Int Soc Sugar Cane Technol 15(1):130–137

    Google Scholar 

  • Kulkarni VM, Bapat VA (2013) Somatic embryogenesis and plant regeneration from cell suspension cultures of Rajeli (AAB), an endangered banana cultivar. J Plant Biochem Biotechnol 22(1):132–137

    Article  CAS  Google Scholar 

  • Kumar V, Chandra S (2014) High frequency somatic embryogenesis and synthetic seed production of the endangered species Swertia chirayita. Biologia 69(2):186–192

    Article  CAS  Google Scholar 

  • Kumar V, Van Staden J (2016) A review of Swertia chirayita (Gentianaceae) as a traditional medicinal plant. Front Pharmacol 6:308

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kunitake H, Koreeda K, Mii M (1995) Morphological and cytological characteristics of protoplast-derived plants of statice (Limonium perezii Hubbard). Sci Hortic 60(3–4):305–312

    Article  Google Scholar 

  • Kwon HJ, Kim HY, Bae KH, Kim SY (2018) Improvement of seed germination in Dysophylla yatabeana, a critically endangered plant species in Korea. Seed Sci Technol 46(2):385–392

    Article  Google Scholar 

  • Lai FM, Lecouteux CG, McKersie BD (1995) Germination of alfalfa (Medicago sativa L.) seeds and desiccated somatic embryos: I. Mobilization of storage reserves. J Plant Physiol 145(4):507–513

    Article  CAS  Google Scholar 

  • Lansdown RV, Knees SG & Patzelt A (2013) Bacopa monnieri. The IUCN Red List of Threatened Species 2013

    Google Scholar 

  • Larkin PJ, Scowcroft WR (1981) Somaclonal variation—a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60(4):197–214

    Article  CAS  PubMed  Google Scholar 

  • Latif Z, Nasir IA, Riazuddin S (2007) Indigenous production of synthetic seeds in Daucus carota. Pak J Bot 39(3):849–855

    Google Scholar 

  • Lee YI (2010) Micropropagation of Cypripedium formosanum Hayata through axillary buds from mature plants. HortScience 45(9):1369–1372

    Article  Google Scholar 

  • Lee M, Phillips RL (1988) The chromosomal basis of somaclonal variation. Annu Rev Plant Physiol Plant Mol Biol 39(1):413–437

    Article  Google Scholar 

  • Lentz KA, Johnson HA (1998) Factors affecting germination of endangered northeastern bulrush, Scirpus ancistrochaetus Schuyler (Cyperaceae). Seed Sci Technol 26(3):733–741

    Google Scholar 

  • Leung AY (1980) Encyclopedia of common natural ingredients used in food, drugs, and cosmetics. Wiley, Chichester

    Google Scholar 

  • Liang KM, Lin ZF, Ren H, Liu N, Zhang QM, Wang J, Wang ZF, Guan LL (2010) Characteristics of sun-and shade-adapted populations of an endangered plant Primulina tabacum Hance. Photosynthetica 48(4):494–506

    Article  CAS  Google Scholar 

  • Liu H, Zhang D, Yang X, Huang Z, Duan S, Wang X (2014) Seed dispersal and germination traits of 70 plant species inhabiting the Gurbantunggut desert in northwest China. Sci World J 2014:346405

    Google Scholar 

  • Ma GH, He CX, Ren H, Zhang QM, Li SJ, Zhang XH, Eric B (2010) Direct somatic embryogenesis and shoot organogenesis from leaf explants of Primulina tabacum. Biol Plant 54(2):361–365

    Article  Google Scholar 

  • Ma XM, Wu CF, Wang GR (2011) Application of artificial seeds in rapid multiplication of Pseudostellaria heterophylla. Afr J Biotechnol 10(70):15744–15748

    Article  CAS  Google Scholar 

  • Ma X, Bucalo K, Determann RO, Cruse-Sanders JM, Pullman GS (2012) Somatic embryogenesis, plant regeneration, and cryopreservation for Torreya taxifolia, a highly endangered coniferous species. In Vitro Cell Dev Biol Plant 48(3):324–334

    Article  CAS  Google Scholar 

  • Mahendran G, Bai VN (2016) Direct somatic embryogenesis of Malaxis densiflora (A. Rich.) Kuntze. J Genet Eng Biotechnol 14(1):77–81

    Article  CAS  PubMed  Google Scholar 

  • Malabadi RB, Van Staden J (2005) Storability and germination of sodium alginate encapsulated somatic embryos derived from the vegetative shoot apices of mature Pinus patula trees. Plant Cell Tissue Organ Cult 82(3):259–265

    Article  CAS  Google Scholar 

  • Mandal J, Pattnaik S, Chand PK (2000) Alginate encapsulation of axillary buds of Ocimum americanum L. (hoary basil), O. Basilicum L. (sweet basil), O. Gratissimum L. (shrubby basil), and O. Sanctum L. (sacred basil). In Vitro Cell Dev Biol Plant 36(4):287–292

    Article  CAS  Google Scholar 

  • Maneerattanarungroj P, Bunnag S, Monthatong M (2007) In vitro conservation of Cleisostoma arietinum (Rchb. f.) Garay, rare Thai orchid species by an encapsulation-dehydration method. Asian J Plant Sci 6(8):1235–1240

    Article  CAS  Google Scholar 

  • Maqsood M, Mujib A, Siddiqui ZH (2012) Synthetic seed development and conversion to plantlet in Catharanthus roseus (L.) G. Don. Biotechnology 11(1):37–43

    Article  CAS  Google Scholar 

  • Marriott P, Sarasan V (2010) Novel micropropagation and weaning methods for the integrated conservation of a critically endangered tree species, Medusagyne oppositifolia. In Vitro Cell Dev Biol Plant 46(6):516–523

    Article  Google Scholar 

  • Martin KP (2003) Clonal propagation, encapsulation and reintroduction of Ipsea malabarica (Reichb. f.) JD Hook., an endangered orchid. In Vitro Cell Dev Biol Plant 39(3):322–326

    Article  CAS  Google Scholar 

  • Martin KP, Pradeep AK (2003) Simple strategy for the in vitro conservation of Ipsea malabarica an endemic and endangered orchid of the Western Ghats of Kerala, India. Plant Cell Tissue Organ Cult 74(2):197–200

    Article  Google Scholar 

  • Martínez-Ruvalcaba A, Chornet E, Rodrigue D (2007) Viscoelastic properties of dispersed chitosan/xanthan hydrogels. Carbohydr Polym 67(4):586–595

    Article  CAS  Google Scholar 

  • Martins DJ (2010) Pollination and seed dispersal in the endangered succulent Euphorbia brevitorta. J East Afr Nat Hist 99(1):9–17

    Article  Google Scholar 

  • Maruyama E, Hosoi Y, Ishii K (2007) Somatic embryogenesis and plant regeneration in yakutanegoyou, Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, an endemic and endangered species in Japan. In Vitro Cell Dev Biol Plant 43(1):28–34

    Article  CAS  Google Scholar 

  • McKersie BD, Senaratna T, Bowley SR, Brown DCW, Krochko JE, Bewley JD (1989) Application of artificial seed technology in the production of hybrid alfalfa (Medicago sativa L.). In Vitro Cell Dev Biol 25(12):1183–1188

    Article  Google Scholar 

  • Merkle SA, Wiecko AT, Watson-Pauley BA (1991) Somatic embryogenesis in American chestnut. Can J For Res 21(11):1698–1701

    Article  Google Scholar 

  • Micheli M, Pellegrino S, Piccioni E, Standardi A (2002) Effects of double encapsulation and coating on synthetic seed conversion in M. 26 apple rootstock. J Microencapsul 19(3):347–356

    Article  CAS  PubMed  Google Scholar 

  • Micheli M, Hafiz IA, Standardi A (2007) Encapsulation of in vitro-derived explants of olive (Olea europaea L. cv. Moraiolo): II. Effects of storage on capsule and derived shoots performance. Sci Hortic 113(3):286–292

    Article  Google Scholar 

  • Mira S, González-Benito ME, Ibars AM, Estrelles E (2011) Dormancy release and seed ageing in the endangered species Silene diclinis. Biodivers Conserv 20(2):345–358

    Article  Google Scholar 

  • Mishra NK, Gupta RK (2017) Effect of distillery effluent on seed germination and vigour index of an endangered medicinal plant Rauwolfia serpentina Benth. Indian J Sci Res 27–31

    Google Scholar 

  • Mohan C, Naresh B, Kumar BK, Reddy V, Manjula P, Keerthi B, Sreekanth D, Manzelat SF, Cherku PD (2017) Micropropagation studies and phytochemical analysis of the endangered tree Commiphora wightii. J Appl Res Med Aromat Plants 6:70–79

    Google Scholar 

  • Mohanty P, Nongkling P, Das MC, Kumaria S, Tandon P (2013) Short-term storage of alginate-encapsulated protocorm-like bodies of Dendrobium nobile Lindl.: an endangered medicinal orchid from North-east India. 3 Biotech 3(3):235–239

    Article  PubMed  Google Scholar 

  • Moon HK, Kim JA, Park SY, Kim YW, Kang HD (2006) Somatic embryogenesis and plantlet formation from a rare and endangered tree species, Oplopanax elatus. J Plant Biol 49(4):320

    Article  CAS  Google Scholar 

  • Morrison RA, Whitaker RJ, Evans DA (1988) Somaclonal variation: its genetic basis and prospects for crop improvement. In: Conn EE (ed) Opportunities for phytochemistry in plant biotechnology. Springer, Boston, pp 1–18

    Google Scholar 

  • Morrison DA, Mcclay K, Porter C, Rish S (1998) The role of the lens in controlling heat-induced breakdown of testa-imposed dormancy in native Australian legumes. Ann Bot 82(1):35–40

    Article  Google Scholar 

  • Mujib A, Banerjee S, Dev Ghosh P (2007) Callus induction, somatic embryogenesis and chromosomal instability in tissue culture raised hippeastrum (Hippeastrum hybridum cv. United Nations). Propag Ornam Plants 7:169–174

    Google Scholar 

  • Muñoz-Concha D, Davey MR (2011) Micropropagation of the endangered Chilean tree, Gomortega keule. In Vitro Cell Dev Biol Plant 47(1):170–175

    Article  Google Scholar 

  • Murashige T (1977) Plant cell and organ cultures as horticultural practices. Symp Tissue Cult Hortic Purp 78:17–30

    Google Scholar 

  • Murthy KSR, Kondamudi R, Karuppusamy S (2012) Microtuberization of Ceropegia spiralis Wight and Ceropegia pusilla Wt. and Arn. Afr J Plant Sci 6(12):21–327

    Google Scholar 

  • Nagesh KS, Shanthamma C, Bhagyalakshmi N (2009) Role of polarity in de novo shoot bud initiation from stem disc explants of Curculigo orchioides Gaertn. and its encapsulation and storability. Acta Physiol Plant 31(4):699–704

    Article  CAS  Google Scholar 

  • Nagesh KS, Shanthamma C, Pullaiah T (2010) Somatic embryogenesis and plant regeneration from callus cultures of Curculigo orchioides Gaertn. Indian J Biotechnol 9:408–413

    CAS  Google Scholar 

  • Natale S (2016) The role of fire in the population dynamics of the threatened plant species Pomaderris walshii and Pomaderris adnata

    Google Scholar 

  • National Medicinal Plant Board (2004) Thirty two prioritized medicinal plants, national informatics centre, ministry of health and family welfare, Department of Ayush, Government of India

    Google Scholar 

  • Necajeva J, Ievinsh G (2013) Seed dormancy and germination of an endangered coastal plant Eryngium maritimum (Apiaceae). Estonian J Ecol 62(2):150

    Article  Google Scholar 

  • Neuschulz EL, Mueller T, Schleuning M, Böhning-Gaese K (2016) Pollination and seed dispersal are the most threatened processes of plant regeneration. Sci Rep 6:29839

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Niiho Y, Yamazaki T, Nakajima Y, Yamamoto T, Ando H, Hirai Y, Toriizuka K, Ida Y (2006) Gastroprotective effects of bitter principles isolated from Gentian root and Swertia herb on experimentally-induced gastric lesions in rats. J Nat Med 60(1):82–88

    Article  CAS  Google Scholar 

  • Nishitha IK, Martix KP, Beegum AS, Madhusoodanan PV (2006) Micropropagation and encapsulation of medicinally important Chonemorpha grandiflora. In Vitro Cell Dev Biol Plant 42(5):385–388

    Article  CAS  Google Scholar 

  • Northcutt C, Davies D, Gagliardo R, Bucalo K, Determann RO, Cruse-Sanders JM, Pullman GS (2012) Germination in vitro, micropropagation, and cryogenic storage for three rare pitcher plants: Sarracenia oreophila (Kearney) Wherry (Federally Endangered), S. leucophylla Raf., and S. purpurea spp. venosa (Raf.) Wherry. HortScience 47(1):74–80

    Article  Google Scholar 

  • Norton S, Lacroix C (1990) Gellan gum gel as entrapment matrix for high temperature fermentation processes: a rheological study. Biotechnol Tech 4(5):351–356

    CAS  Google Scholar 

  • Nower AA (2014) In vitro propagation and synthetic seeds production: an efficient methods for Stevia rebaudiana Bertoni. Sugar Tech 16(1):100–108

    Article  CAS  Google Scholar 

  • Oates MR, de Lange PJ (1998) Chordospartium muritai. IUCN Red List Threatened Species. IUCN. 1998:e.T32309A9694330. https://doi.org/10.2305/IUCN.UK.1998.RLTS.T32309A9694330.en. Retrieved 20 Dec 2017

  • Offord CA, Tyler JL (2009) In vitro propagation of Pimelea spicata R. Br (Thymelaeaceae), an endangered species of the Sydney region, Australia. Plant Cell Tissue Organ Cult 98(1):19–23

    Article  CAS  Google Scholar 

  • Onishi N, Sakamoto Y, Hirosawa T (1994) Synthetic seeds as an application of mass production of somatic embryos. Plant Cell Tissue Organ Cult 39(2):137–145

    Article  Google Scholar 

  • Ouyang Y, Chen Y, Lü J, Da Silva JAT, Zhang X, Ma G (2016) Somatic embryogenesis and enhanced shoot organogenesis in Metabriggsia ovalifolia WT Wang. Sci Rep 6:24662

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Pandey A, Chand S (2005) Efficient plant regeneration from encapsulated somatic embryos of Hyoscyamus muticus L. Indian J Biotechnol 4:546–550

    CAS  Google Scholar 

  • Pandey M, Dhar U, Sher Singh S, Mrudul Vijay S, Shubhada Ratnakar T (2011) Recurrent somatic embryogenesis and plant regeneration in Angelica glauca Edgew., a critically endangered medicinal plant of the Western Himalaya. J Hortic Sci Biotechnol 86(5):493–498

    Article  Google Scholar 

  • Parsons LS, Zedler JB (1997) Factors affecting reestablishment of an endangered annual plant at a California salt marsh. Ecol Appl 7(1):253–267

    Article  Google Scholar 

  • Pattnaik SK, Sahoo Y, Chand PK (1995) Efficient plant retrieval from alginate-encapsulated vegetative buds of mature mulberry trees. Sci Hortic 61(3–4):227–239

    Article  Google Scholar 

  • Patykowski J, Dell M, Gibson M (2016) Germination ecology and seed dispersal of a critically endangered plant: a case study of Pomaderris vacciniifolia (Round-Leaf Pomaderris). PLoS One 11(8):e0161665

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Paunescu A (2009) Hormonal regulation of somatic embryogenesis and organogenesis in Alyssum borzaeanum Nyár. in vitro culture system. Electron J Biol 5(3):45–48

    Google Scholar 

  • Pavendan P, Rajasekaran CS (2011) Effect of different concentrations of plant growth regulators for micropropagation of Eugenia singampattiana Beddome endangered tree species. Res J Bot 6(3):122–127

    Article  CAS  Google Scholar 

  • Pavlik BM, Ferguson N, Nelson M (1993) Assessing limitations on the growth of endangered plant populations. II. Seed production and seed bank dynamics of Erysimum capitatum ssp. angustatum and Oenothera deltoides ssp. howellii. Biol Conserv 65(3):267–278

    Article  Google Scholar 

  • Peng SL, Cheng WC (2002) Rare and endangered plants in Guangdong. Science, Beijing

    Google Scholar 

  • Pérez-Bermúdez P, Seitz HU, Gavidia I (2002) A protocol for rapid micropropagation of endangered Isoplexis. In Vitro Cell Dev Biol Plant 38(2):178–182

    Article  CAS  Google Scholar 

  • Peschke VM, Phillips RL (1992) Genetic implications of somaclonal variation in plants. In: Vasil IK (ed) Advances in genetics, vol 30. Academic, New York, pp 41–75

    Google Scholar 

  • Phartyal SS, Kondo T, Hoshino Y, Baskin CC, Baskin JM (2009) Morphological dormancy in seeds of the autumn-germinating shrub Lonicera caerulea var. emphyllocalyx (Caprifoliaceae). Plant Species Biol 24(1):20–26

    Article  Google Scholar 

  • Piątczak E, Wysokińska H (2013) Encapsulation of Centaurium erythraea Rafn–an efficient method for regeneration of transgenic plants. Acta Biol Cracov Ser Bot 55(2):37–44

    Google Scholar 

  • Piccioni E, Standardi A (1995) Encapsulation of micropropagated buds of six woody species. Plant Cell Tissue Organ Cult 42(3):221–226

    Article  Google Scholar 

  • Pietsch GM, Anderson NO (2007) Epigenetic variation in tissue cultured Gaura lindheimeri. Plant Cell Tissue Organ Cult 89(2–3):91–103

    Article  Google Scholar 

  • Qiao Q, Chen H, Xing F, Wang F, Zhong W, Wen X, Hou X (2012) Pollination ecology of Bretschneidera sinensis (hemsley), a rare and endangered tree in China. Pak J Bot 44(6):1897–1903

    Google Scholar 

  • Rathwell R, Popova E, Shukla MR, Saxena PK (2016) Development of cryopreservation methods for cherry birch (Betula lenta L.), an endangered tree species in Canada. Can J For Res 46(11):1284–1292

    Article  CAS  Google Scholar 

  • Rawat JM, Rawat B, Mehrotra S (2013) Plant regeneration, genetic fidelity, and active ingredient content of encapsulated hairy roots of Picrorhiza kurrooa Royle ex Benth. Biotechnol Lett 35(6):961–968

    Article  CAS  PubMed  Google Scholar 

  • Reddy MC, Murthy KSR, Pullaiah T (2012) Synthetic seeds: a review in agriculture and forestry. Afr J Biotechnol 11(78):14254–14275

    Google Scholar 

  • Redenbaugh K (1990) Application of artificial seed to tropical crops. HortScience 25(3):251–255

    Article  Google Scholar 

  • Redenbaugh K (1993) Synthetic seeds: applications of synthetic seeds to crop improvement. CRC, Boca Raton

    Google Scholar 

  • Redenbaugh K, Fujii JA, Slade D (1988) Encapsulated plant embryos. In: Mizrahi A (ed) Advances in biotechnological processes. Liss, New York

    Google Scholar 

  • Repunte VP, Taya M, Tone S (1996) Conservation of root regeneration potential of cell aggregates from horseradish hairy roots used as artificial seeds. J Chem Eng Jpn 29(5):874–880

    Article  CAS  Google Scholar 

  • Reuveni O, Israeli Y (1989) Measures to reduce somaclonal variation in in vitro propagated bananas. In: International Symposium on the Culture of Subtropical and Tropical Fruits and Crops, vol 275, pp 307–314

    Google Scholar 

  • Rizvi MZ, Kukreja AK, Bisht NS (2010) In vitro propagation of an endangered medicinal herb Chlorophytum borivilianum Sant. et Fernand. through somatic embryogenesis. Physiol Mol Biol Plants 16(3):249–257

    Article  PubMed  PubMed Central  Google Scholar 

  • Rodrigues PHV, Tulmann Neto A, Cassieri Neto P, Mendes BMJ (1997) Influence of the number of subcultures on somaclonal variation in micropropagated nanicao (Musa spp., AAA Group). In II International Symposium on Banana: I International Symposium on Banana in the Subtropics 490, pp 469–474, November 1997

    Google Scholar 

  • Rojas-Sandoval J, Meléndez-Ackerman E (2012) Factors affecting establishment success of the endangered Caribbean cactus Harrisia portoricensis (Cactaceae). Rev Biol Trop 60(2):867–879

    Article  PubMed  Google Scholar 

  • Russo A, Borrelli F (2005) Bacopa monniera, a reputed nootropic plant: an overview. Phytomedicine 12(4):305–317

    Article  CAS  PubMed  Google Scholar 

  • Sadeq MA, Pathak MR, Salih AA, Abido M, Abahussain A (2014) Somatic embryogenesis and organogenesis for regeneration of endangered multipurpose desert plant Leptadenia pyrotechnica Forsk. Decne in the Kingdom of Bahrain. Am J Plant Sci 5(15):2342

    Article  Google Scholar 

  • Sahai A, Shahzad A, Anis M (2010) High frequency plant production via shoot organogenesis and somatic embryogenesis from callus in Tylophora indica, an endangered plant species. Turk J Bot 34(1):11–20

    CAS  Google Scholar 

  • Sakamoto Y, Onishi N, Hirosawa T (1995) Delivery systems for tissue culture by encapsulation. In: Automation and environmental control in plant tissue culture. Springer, Dordrecht, pp 215–243

    Chapter  Google Scholar 

  • Salama A, Shukla MR, Popova E, Fisk NS, Jones MP, Saxena PK (2018) In vitro propagation and reintroduction of golden paintbrush (Castilleja levisecta), a critically imperilled plant species. Can J Plant Sci 98(3):762–770

    Article  CAS  Google Scholar 

  • Sarmah DK, Borthakur M, Borua PK (2010) Artificial seed production from encapsulated PLBs regenerated from leaf base of Vanda coerulea Grifft. ex. Lindl. – an endangered orchid. Curr Sci 98:686–690

    CAS  Google Scholar 

  • Saradha M, Samydurai P (2018) Micropropagation of critically endangered medicinal plant. Int J Curr Res 10(07):71698–71703

    CAS  Google Scholar 

  • Segura F, Vicente MJ, Franco JA, Martínez-Sánchez JJ (2015) Effects of maternal environmental factors on physical dormancy of Astragalus nitidiflorus seeds (Fabaceae), a critically endangered species of SE Spain. Flora 216:71–76

    Article  Google Scholar 

  • Shah JD (1965) Studies in growth and ecology of B. monniera (L.) Pennl. a medicinal herb. a Ph.D. thesis, Banaras Hindu University, Varanasi, India

    Google Scholar 

  • Sharma SK, Bryan GJ, Winfield MO, Millam S (2007) Stability of potato (Solanum tuberosum L.) plants regenerated via somatic embryos, axillary bud proliferated shoots, microtubers and true potato seeds: a comparative phenotypic, cytogenetic and molecular assessment. Planta 226(6):1449–1458

    Article  CAS  PubMed  Google Scholar 

  • Sharma M, Raina H, Mallubhotla SP, Ahuja A, Vid RVSKV (2012) Synthetic seeds a viable approach for conservation and propagation of phytoremediant herb: Bacopa monnieri (L.) WETTST. J Environ Res Dev 7:399–404

    Google Scholar 

  • Sharma A, Kaur R, Sharma N (2014a) In vitro morphogenic response of different explants of Gentiana kurroo Royle from Western Himalayas—an endangered medicinal plant. Physiol Mol Biol Plants 20(2):249–256

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sharma S, Shahzad A, Kumar J, Anis M (2014b) In vitro propagation and synthetic seed production of scarlet salvia (Salvia splendens). Rendiconti Lincei 25(3):359–368

    Article  Google Scholar 

  • Sharmin SA, Alam M, Sheikh M, Islam M, Sarker KK, Khalekuzzaman M, Haque M, Alam MF, Alam I (2014) Somatic embryogenesis and plant regeneration in Wedelia calendulacea Less. An endangered medicinal plant. Braz Arch Biol Technol 57(3):394–401

    Article  CAS  Google Scholar 

  • Shepard JF, Bidney D, Shahin E (1980) Potato protoplasts in crop improvement. Science 208(4439):17–24

    Article  CAS  PubMed  Google Scholar 

  • Sherif NA, Benjamin JF, Kumar TS, Rao MV (2018) Somatic embryogenesis, acclimatization and genetic homogeneity assessment of regenerated plantlets of Anoectochilus elatus Lindl., an endangered terrestrial jewel orchid. Plant Cell Tissue Organ Cult 132(2):303–316

    Article  CAS  Google Scholar 

  • Shibli RA, Duwayri MA, Sawwan JS, Shatnawi MA, Al-Qudah TS (2012) Regeneration via somatic embryogenesis of the endangered wild arum (Arum palaestinum). In Vitro Cell Dev Biol Plant 48(3):335–340

    Article  CAS  Google Scholar 

  • Siddique I, Bukhari NAW (2018) Synthetic seed production by encapsulating nodal segment of Capparis decidua (Forsk.), in vitro regrowth of plantlets and their physio biochemical studies. Agrofor Syst 92:1–9

    Article  Google Scholar 

  • Singh AK, Sharma M, Varshney R, Agarwal SS, Bansal KC (2006) Plant regeneration from alginate-encapsulated shoot tips of Phylianthus amarus Schum and Thonn, a medicinally important plant species. In Vitro Cell Dev Biol Plant 42(2):109–113

    Article  CAS  Google Scholar 

  • Siong PK, Mohajer S, Taha RM (2012) Production of artificial seeds derived from encapsulated in vitro micro shoots of cauliflower, Brassica oleracea var. botrytis. Rom Biotechnol Lett 17(4):7549–7556

    CAS  Google Scholar 

  • Slazak B, Sliwinska E, Saługa M, Ronikier M, Bujak J, Słomka A, Göransson U, Kuta E (2015) Micropropagation of Viola uliginosa (Violaceae) for endangered species conservation and for somaclonal variation-enhanced cyclotide biosynthesis. Plant Cell Tissue Organ Cult 120(1):179–190

    Article  CAS  Google Scholar 

  • Smith RH (2012) Plant tissue culture: techniques and experiments. Academic, New York

    Google Scholar 

  • Soneji J, Rao P, Mhatre M (2002) Germination of synthetic seeds of pineapple (Ananas comosus L. Merr.). Plant Cell Rep 20(10):891–894

    Article  CAS  Google Scholar 

  • Sotes GJ, Bustamante RO, Henríquez CA (2018) Leaf litter is essential for seed survival of the endemic endangered tree Pouteria splendens (Sapotaceae) from central Chile. Web Ecol 18(1):1–5

    Article  Google Scholar 

  • Srinivasarao C, Soni A, Dulloo ME, Naithani SC (2010) Overcoming physiological dormancy in Ceropegia odorata seeds, an endangered rare species, with GA3, H2O2 and KNO3. Seed Sci Technol 38(2):341–347

    Article  Google Scholar 

  • Standardi A, Piccioni E (1998) Recent perspectives on synthetic seed technology using nonembryogenic in vitro–derived explants. Int J Plant Sci 159(6):968–978

    Google Scholar 

  • Subramaniam S, Sinniah UR, Khoddamzadeh AA, Periasamy S, James JJ (2011) Fundamental concept of cryopreservation using Dendrobium sonia-17 protocorm-like bodies by encapsulation-dehydration technique. Afr J Biotechnol 10(19):3902–3907

    Google Scholar 

  • Sundaram S, Ahmad IZ, Dwivedi P (2011) Study of different stages of somatic embryogenesis in a medicinal plant, madar (Calotropis procera). Res J Bot 6:1–10

    Article  CAS  Google Scholar 

  • Suprasanna P, Ganapathi TR, Bapat VA (2006) Synthetic seeds technology. In: Basra AS (ed) Handbook of seed science and technology. CRC, New York, pp 227–267

    Google Scholar 

  • Tabassum B, Nasir IA, Farooq AM, Rehman Z, Latif Z, Husnain T (2010) Viability assessment of in vitro produced synthetic seeds of cucumber. Afr J Biotechnol 9(42):7026–7032

    CAS  Google Scholar 

  • Tabin T, Shrivastava K (2014) Factors affecting seed germination and establishment of critically endangered Aquilaria malaccensis (Thymelaeaceae). Asian J Plant Sci Res 4(6):41–46

    Google Scholar 

  • Taha RM, Hasbullah NA, Awal A (2008) Production of synthetic seeds from micro shoots and somatic embryos of Gerbera jamesonii Bolus ex. Hook f. In: 6th international symposium on in vitro culture and horticultural breeding, August 2008, vol 829, pp 91–98

    Google Scholar 

  • Tapingkae T, Zulkarnain Z, Kawaguchi M, Ikeda T, Taji A (2012) Somatic (asexual) procedures (haploids, protoplasts, cell selection) and their applications. In: Altman A, Hasegawa PM (eds) Plant biotechnology and agriculture. Academic, Oxford, pp 141–162

    Chapter  Google Scholar 

  • Thomas TD, Philip B (2005) Thidiazuron-induced high-frequency shoot organogenesis from leaf-derived callus of ia medicinal climber, Tylophora Indica (Burm. F.) merrill. In Vitro Cell Dev Biol Plant 41(2):124–128

    Article  CAS  Google Scholar 

  • Tiwari V, Tiwari KN, Singh BD (2001) Comparative studies of cytokinins on in vitro propagation of Bacopa monniera. Plant Cell Tissue Organ Cult 66(1):9–16

    Article  CAS  Google Scholar 

  • Traveset A, Riera N (2005) Disruption of a plant-lizard seed dispersal system and its ecological effects on a threatened endemic plant in the Balearic Islands. Conserv Biol 19(2):421–431

    Article  Google Scholar 

  • Tremblay L, Levasseur C, Tremblay FM (1999) Frequency of somaclonal variation in plants of black spruce (Picea mariana, Pinaceae) and white spruce (P. glauca, Pinaceae) derived from somatic embryogenesis and identification of some factors involved in genetic instability. Am J Bot 86(10):1373–1381

    Article  CAS  PubMed  Google Scholar 

  • Turner SR, Merritt DJ, Baskin JM, Baskin CC, Dixon KW (2006) Combinational dormancy in seeds of the Western Australian endemic species Diplopeltis huegelii (Sapindaceae). Aust J Bot 54(6):565–570

    Article  Google Scholar 

  • Uozumi N, Kobayashi T (1995) Artificial seed production through encapsulation of hairy root and shoot tips. In: Bajaj YPS (ed) Somatic embryogenesis and synthetic seed I. Springer, Berlin, pp 170–180

    Chapter  Google Scholar 

  • Veiga-Barbosa L, Ruiz C, Correa EC, Pérez-García F (2016) Dormancy imposed by a tough seed coat in Malvella sherardiana (Malvaceae), a highly threatened species of Spain. Bot Lett 163(3):321–327

    Article  Google Scholar 

  • Verma M, Bansal YK (2012) Induction of somatic embryogenesis in endangered butterfly ginger Hedychium coronarium J. Koenig. Indian J Exp Biol 50(12):904–909

    Google Scholar 

  • Vorlop KD, Klein J (1981) Formation of spherical chitosan biocatalysts by ionotropic gelation. Biotechnol Lett 3(1):9–14

    Article  CAS  Google Scholar 

  • Wang Y, Chen F, Wang Y, Li X, Liang H (2014) Efficient somatic embryogenesis and plant regeneration from immature embryos of Tapiscia sinensis Oliv., an endemic and endangered species in China. HortScience 49(12):1558–1562

    Article  Google Scholar 

  • Wattimena GA (1984) Micropropagation as an alternative technology for potato production in Indonesia. PhD dissertation, University of Wisconsin, Madison

    Google Scholar 

  • Whiteley SE, Bunn E, Menon A, Mancera RL, Turner SR (2016) Ex situ conservation of the endangered species Androcalva perlaria (Malvaceae) by micropropagation and cryopreservation. Plant Cell Tissue Organ Cult 125(2):341–352

    Article  CAS  Google Scholar 

  • Wilkinson TIM, Wetten A, Prychid C, Fay MF (2003) Suitability of cryopreservation for the long-term storage of rare and endangered plant species: a case history for Cosmos atrosanguineus. Ann Bot 91(1):65–74

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Williams PA, Norton DA, Nicholas JM (1996) Germination and seedling growth of an endangered native broom, Chordospartium muritai AW Purdie (Fabaceae), found in Marlborough, South Island, New Zealand. N Z J Bot 34(2):199–204

    Article  Google Scholar 

  • Winkelmann T (2016) Somatic versus zygotic embryogenesis: learning from seeds. In: Germana MA, Lambardi M (eds) In vitro embryogenesis in higher plants. Humana, New York, pp 25–46

    Chapter  Google Scholar 

  • Winkelmann T, Meyer L, Serek M (2004) Desiccation of somatic embryos of Cyclamen persicum Mill. J Hortic Sci Biotechnol 79(3):479–483

    Article  Google Scholar 

  • Wu K, Zeng S, Lin D, da Silva JAT, Bu Z, Zhang J, Duan J (2014) In vitro propagation and reintroduction of the endangered Renanthera imschootiana Rolfe. PLoS One 9(10):e110033

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Yadav K, Aggarwal A, Singh N (2012) Actions for ex situ conservation of Gloriosa superba L. – an endangered ornamental cum medicinal plant. J Crop Sci Biotechnol 15(4):297–303

    Article  Google Scholar 

  • Yeung EC (2017) A perspective on orchid seed and protocorm development. Bot Stud 58(1):33

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Yussof AIM, Wafa SNA, Taha RM (2011) Plant regeneration and synthetic seeds production of Brassica oleracea var. italic. In: I International symposium on sustainable vegetable production in Southeast Asia, March 2011, vol 958, pp 179–185

    Google Scholar 

  • Zheng YL, Sun WB (2009) Seed germination of Huagaimu, a critically endangered plant endemic to southeastern Yunnan, China. HortTechnology 19(2):427–431

    Article  CAS  Google Scholar 

  • Zych M, Furmanowa M, Krajewska-Patan A, Łowicka A, Dreger M, Mendlewska S (2005) Micropropagation of Rhodiola kirilowii plants using encapsulated axillary buds and callus. Acta Biol Cracov Ser Bot 47(2):83–87

    Google Scholar 

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Authors and Affiliations

  1. Gosling Research Institute for Plant Preservation (GRIPP), Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada

    Akansha Saxena, Mukund Shukla & Praveen Saxena

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  1. Akansha Saxena
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  2. Mukund Shukla
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  3. Praveen Saxena
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Correspondence to Praveen Saxena .

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  1. Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

    Mohammad Faisal

  2. Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

    Abdulrahman A. Alatar

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Saxena, A., Shukla, M., Saxena, P. (2019). Synthetic Seeds: Relevance to Endangered Germplasm Conservation In Vitro. In: Faisal, M., Alatar, A. (eds) Synthetic Seeds . Springer, Cham. https://doi.org/10.1007/978-3-030-24631-0_2

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