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Introduction to Microscopy

  • Giuseppe Di DomenicoEmail author
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Part of the Springer Theses book series (Springer Theses)

Abstract

In this chapter we describe the basics and the state of the art of optical microscopy, the governing equation, the diffraction limit, image formation and the main criteria used for the analysis of imaging systems. In this framework, we also introduce the main concepts of super resolution, methods that can overcome the resolution limit using physical or numerical techniques, structured illumination microscopy, in which the sample is illuminated under different spatially inhomogeneous light fields and fluorescence microscopy, that images specific chemical components of a sample thanks to a selective staining using fluorophores.

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Authors and Affiliations

  1. 1.School of Electrical EngineeringTel Aviv UniversityTel AvivIsrael

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