Breeding and Improvement of the Oil Palm Interspecific Hybrids via Molecular Cytogenetics

  • M. Madon
  • H. M. N. Nordiana
  • Z. Muhammad Azwan
  • N. M. ZakiEmail author
Part of the Compendium of Plant Genomes book series (CPG)


Breeding and improvement of the oil palm interspecific hybrids can take long time and involve multiple resources due to oil palm having a long breeding cycle, which needs large areas to be planted, intensive labour, and high maintenance cost. However, efforts can be optimized via molecular cytogenetics such as fluorescence in situ hybridization (FISH) method to locate DNA sequences of interest on chromosomes and genomic in situ hybridization (GISH) which is used to discriminate chromosomes of E. guineensis and E. oleifera by ascertaining the amount of oleifera genomes introgressed into O×G hybrids and their backcrosses and to look for introgressed chromosomes. The oil palm planted area worldwide is increasing continuously. Therefore, it is necessary to be prepared with a diversified planting material to avoid major economic loss if ever the disease arises in Malaysia.


Interspecific hybrids Oil palm Molecular cytogenetics Fluorescence in situ hybridization 


  1. Anamthawat-Jonsson K, Bodvarsdottir SK (1998) Meiosis of wheat × lymegrass hybrids. Chromosome Res 6:339–343CrossRefGoogle Scholar
  2. Barcelos E, Amblard P (2017) Elaeisoleifera genetic resources. In: Soh AC, Mayes S, Roberts J (eds) Oil palm breeding. Genetics and genomics, pp 45–48Google Scholar
  3. Billotte N, Jourjon MF, Marseillac N, Berger A, Flori A, Asmady H, Adon B, Singh R, Nouy B, Potier F, Cheah SC, Rohde W, Ritter E, Courtois B, Charrier A, Margin B (2010) QTL detection by multi-parent linkage mapping in oil palm (Elaeis guineensis Jacq.). Theor Appl Genet 120(8):1673–1687Google Scholar
  4. Castilho A, Vershinin AV, Heslop-Harrison JS (2000) Repetitive DNA and the chromosomes in the genome of oil palm (Elaeis guineensis). Ann Bot 856:837–844CrossRefGoogle Scholar
  5. Hardon JJ, Tan GY (1969) Interspecific hybrids in the genus Elaeis I. Crossability, cytogenetics and fertility of F1 hybrids of E. guineensis × E. oleifera. Euphytica 18:372–379CrossRefGoogle Scholar
  6. Harper ME, Ulrich A, Saunders GF (1981) Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Proc Natl Acad Sci USA 78:4458–4460CrossRefGoogle Scholar
  7. Hartley CWS (1988) The oil palm (Elaeis guineensis Jacq.) Longman Wiley, New YorkGoogle Scholar
  8. Jiang J, Gill BS (1994) Non-isotopic in situ hybridization and plant genome mapping: the first 10 years. Genome 37:717–725CrossRefGoogle Scholar
  9. Kushairi A, Singh R, Ong-Abdullah M (2017) The oil palm industry in Malaysia: thriving with transformative technologies. J Oil Palm Res 29(4):431–439Google Scholar
  10. Kushairi A, Soh KL, Azman I, Hishamuddin Elina, Ong-Abdullah M, Izuddin Zainal BidinMohd Noor, Razmah G, ShamalaSundram Ghulam Kadir, Parveez Ahmad (2018) Oil palm economic performance in Malaysia and R&D progress in 2017. J Oil Palm Res 30(2):163–195Google Scholar
  11. Langer-Safer PR, Levine M, Ward DC (1982) Immunological method for mapping genes on Drosophila polytene chromosomes. Proc Natl Acad Sci USA 79:4381–4385CrossRefGoogle Scholar
  12. Levsky JM, Singer RH (2003) Fluorescence in situ hybridization: past, present and future. J Cell Sci 116:2833–2838Google Scholar
  13. Madon M, Clyde MM, Cheah SC (1996) Fluorescence in situ hybridization of rRNA probe to Elaeis guineensis (tenera) chromosomes. Elaeis 8(1):29–36Google Scholar
  14. Madon M, Clyde MM, Cheah SC (1998) Cytological analysis of Elaeis guineensis and Elaeis oleifera chromosomes. J Oil Palm Res 10(1):68–91Google Scholar
  15. Madon M, Clyde MM, Cheah SC (1999) Application of genomic in situ hybridization (GISH) on Elaeis hybrids. J Oil Palm Res (Spec Issue): 74–80Google Scholar
  16. Madon M, Arulandoo X, Sritharan K, Nordiana HMN, Muhammad Azwan Z, Mohd Zaki N (2018) Short communication: genomic constitution of oil palm interspecific hybrid crosses monitored by genomic in situ hybridization (GISH). J Oil Palm Res (accepted)Google Scholar
  17. Mayes S, Soh AC, Roberts J (2017) Genetic resources. introductory overview. In: Soh AC, Mayes S, Roberts J (eds) Oil palm breeding. Genetics and genomics, pp 21–23Google Scholar
  18. Obasola CO, Obesesan IO, Opute FI (1976) Breeding of short-stemmed oil palm in Nigeria. In: Proceedings of international agriculture oil palm conference, Kuala Lumpur, 26–28 MayGoogle Scholar
  19. Orgaard M, Heslop-Harrison JS (1994) Investigations of genome relationships between Leymus, Psathyrostachys and Hordeum inferred by genomic DNA: DNA in situ hybridization. Ann Bot 73:195–203CrossRefGoogle Scholar
  20. Parokonny AS, Marshall JA, Bennett MD, Cocking EC, Davey MR, Power JB (1997) Homoeologous pairing and recombination in backcross derivatives of tomato somatic hybrids [Lycopersiconesculentum (+) L. peruvianum]. Theor Appl Genet 94:713–723CrossRefGoogle Scholar
  21. Rajanaidu N, Mohd Din A, Marhalil M, Abdullah N, Ong-Abdullah M, Ahmad Malike F, Abu Bakar NA, Libin A, Yaakub Z, Mustafa S, Ithnin M, Kushairi A (2017) Prospection, conservation and the broadening of the genetic base in oil palm. In: Soh AC, Mayes S, Roberts J (eds) Oil palm breeding. Genetics and genomics, pp 27–45Google Scholar
  22. Ramzan, F, Younis A, Lim K-B (2017) Application of genomic in situ hybridization in horticultural science. Int J Genomics Article ID 7561909, 12 pGoogle Scholar
  23. Rance KA, Mayes S, Price Z, Zack PL, Corley RHV (2001) Quantitative trait loci for yield components in oil palm (Elaeis guineensis Jacq.). Theor Appl Genet 103:1302–1310CrossRefGoogle Scholar
  24. Rayburn AL, Gill BS (1985) Use of biotin-labelled probes to map specific DNA sequences on wheat chromosomes. Heredity 76:78–81CrossRefGoogle Scholar
  25. Soh AC, Mayes S, Roberts J (2017) Introduction to the oil palm crop. In: Soh AC, Mayes S, Roberts J (eds) Oil palm breeding. Genetics and genomics, pp 1–6Google Scholar
  26. Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1989) In situ localization of parental genomes in a wide hybrid. Ann Bot 64:315–324CrossRefGoogle Scholar
  27. Schwendimen J, Pallares P, Amblard P, Baudouin L (1983) Analysis of fertility during bunch development in the interspecific oil palm hybrid Elaeismelanococca × E. guineensis. Oléagineux 38(7):411–420Google Scholar
  28. Singh R, Tan SG, Panandam GM, Rahimah AR, Ooi LCL, Low ETL, Sharma M, Jansen J, Cheah SC (2009) Mapping quantitative trait loci (QTLs) for fatty acid composition in an interspecific cross of oil palm. BMC Plant Biol 9:114CrossRefGoogle Scholar
  29. Singh R, Ong-Abdullah M, Low ETL, Manaf MAA, Rosli R, Nookiah R, Ooi LCL, Ooi SE, Chan KL, Halim MA, Azizi N, Nagappan J, Bacher B, Lakey N, Smith SW, He D, Hogan M, Budiman MA, Lee EK, Desalle R, Kudrna D, Goicoechea JL, Wing RA, Wilson RK, Fulton RS, Ordway JM, Martienssen RA, Sambanthamurthi R (2013) Oil palm genome sequence reveals divergence of interfertile species in old and new worlds. Nature 500(7462):335–339CrossRefGoogle Scholar
  30. Ting NC, Jansen J, Mayes S, Massawe F, Sambanthamurthi R, Ooi LCL, Chin CW, Arulandoo X, Seng TY, Syed Alwee SSR, Ithnin M, Singh R (2014) High density SNP and SSR-based genetic maps of two independent oil palm hybrids. BMC Genom 15:309–320CrossRefGoogle Scholar
  31. Torres GA, Sarria GA, Martinez G, Varon F, Drenth A, Guest DI (2016) Bud rot caused by Phytophthora palmivora: a destructive emerging disease of oil palm. Phytopathology 106:320–329CrossRefGoogle Scholar
  32. Zaki MN, Singh R, Nordiana HMN, Zulkifli MA, Steven WS, Schwarzacher T, Madon M, Heslop-Harrison JS (2017) Short communication: towards development of Elaeis guineensis chromosome-arm specific markers and their utility across the Elaeis genus. J Oil Palm Res 29(4):594–599Google Scholar

Copyright information

© Springer Nature Switzerland AG 2020

Authors and Affiliations

  • M. Madon
    • 1
  • H. M. N. Nordiana
    • 1
  • Z. Muhammad Azwan
    • 1
  • N. M. Zaki
    • 1
    Email author
  1. 1.Malaysian Palm Oil BoardKajangMalaysia

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