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Assessing the Advantages, Limitations and Potential of Human Primary Prostate Epithelial Cells as a Pre-clinical Model for Prostate Cancer Research

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Human Cell Transformation

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1164))

Abstract

Choosing an appropriate cell model(s) is the first decision to be made before starting a new project or programme of study. Here, we address the rationale that can be behind this decision and we summarize the current cell models that are used to study prostate cancer. Researchers face the challenge of choosing a model that recapitulates the complexity and heterogeneity of prostate cancer. The use of primary prostate epithelial cells cultured from patient tissue is discussed, and the necessity for close clinical-academic collaboration in order to do this is highlighted. Finally, a novel quantitative phase imaging technique is described, along with the potential for cell characterization to not only include gene expression and protein markers but also morphological features, cell behaviour and kinetic activity.

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Acknowledgements

This work was supported by Prostate Cancer UK through a Research Innovation Award (RIA15-ST2-022).

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Authors and Affiliations

  1. Cancer Research Unit, Department of Biology, University of York, York, UK

    Fiona M. Frame, Amanda R. Noble, Peter O’Toole, Jo Marrison, Timothy Godden, Andrew O’Brien & Norman J. Maitland

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  1. Fiona M. Frame
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  2. Amanda R. Noble
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  3. Peter O’Toole
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  4. Jo Marrison
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  5. Timothy Godden
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  6. Andrew O’Brien
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  7. Norman J. Maitland
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Correspondence to Fiona M. Frame .

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Editors and Affiliations

  1. Department of Surgery, Uniformed Services University of the Health Sciences, Bethesda, MD, USA

    Johng S. Rhim

  2. Georgetown University Medical Center, Washington, DC, USA

    Anatoly Dritschilo

  3. Department of Medicine, McGill University Health Centre, Montréal, QC, Canada

    Richard Kremer

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Frame, F.M. et al. (2019). Assessing the Advantages, Limitations and Potential of Human Primary Prostate Epithelial Cells as a Pre-clinical Model for Prostate Cancer Research. In: Rhim, J., Dritschilo, A., Kremer, R. (eds) Human Cell Transformation. Advances in Experimental Medicine and Biology, vol 1164. Springer, Cham. https://doi.org/10.1007/978-3-030-22254-3_9

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