Cryopreservation of Oocytes and Embryos
- 566 Downloads
The cryopreservation as a broad discipline entails techniques that permits freezing and freeze-thawing or vitrification-warming of biological materials with minimal loss in their biological functions. Cryopreservation is an integral component of modern biomedical sciences, assisted reproduction and conservation biology, and a fundamental subject itself. Somatic cells, stem cells, primordial germ cells, oocytes, embryos and ovarian tissue preserved aptly under ultralow temperature can endure preservation for unlimited time with almost negligible loss in metabolic and genetic eminence. Compared to conventional slow-freezing cryopreservation techniques, the emphasis is on simpler methods of cryopreservation using vitrification.
Cryopreservation is an integral component of biomedical sciences, agriculture, and animal-assisted reproduction
In view of limitations of slow freezing, alternative methods are developed
Vitrification is a cheaper and efficient method of cryopreservation.
KeywordsCryopreservation Vitrification Bio-banking Cryoprotective agents Livestock applications
- Canesin HS, Brom-de-Luna JG, Choi YH, Pereira AM, Macedo GG, Hinrichs K (2018) Vitrification of germinal-vesicle stage equine oocytes: effect of cryoprotectant exposure time on in-vitro embryo production. Cryobiology 81:185–191. https://doi.org/10.1016/j.cryobiol.2018.01.001 (Epub 2018 Jan 3)PubMedCrossRefGoogle Scholar
- Fathi M, Moawad AR, Badr MR (2018) Production of blastocysts following in vitro maturation and fertilization of dromedary camel oocytes vitrified at the germinal vesicle stage. PLoS One 13(3):e0194602. https://doi.org/10.1371/journal.pone.0194602 (eCollection 2018)PubMedPubMedCentralCrossRefGoogle Scholar
- Gao HH, Li JT, Liu JJ, Yang QA, Zhang JM (2017) Autophagy inhibition of immature oocytes during vitrification-warming and in vitro mature activates apoptosis via caspase-9 and -12 pathway. Eur J Obstet Gynecol Reprod Biol 217:89–93. https://doi.org/10.1016/j.ejogrb.2017.08.029 (Epub 2017 Aug 24)PubMedCrossRefGoogle Scholar
- Gautam SK, Singh B, Verma V, Palta P, Chauhan MS, Manik RS (2009) In vitro production of blastocysts from vitrified-warmed abattoir-derived buffalo (Bubalus bubalis) immature oocytes. Indian J Dairy Sci 62:495–502Google Scholar
- Landa V, Teplá O (1990) Cryopreservation of mouse 8-cell embryos in microdrops. Folia Biol (Praha) 36(3–4):153–158Google Scholar
- Mullen SF, Fahy GM (2012) A chronologic review of mature oocyte vitrification research in cattle, pigs, and sheep. Theriogenology 78(8):1709–1719. https://doi.org/10.1016/j.theriogenology.2012.06.008PubMedCrossRefGoogle Scholar