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Digital PCR

  • Birbal SinghEmail author
  • Gorakh Mal
  • Sanjeev K. Gautam
  • Manishi Mukesh
Chapter
  • 560 Downloads

Abstract

Precise detection of mutations is important for a variety of clinical and research applications. The digital PCR offers precise quantification of nucleic acids, viz. DNA, cDNA and RNA, and mutations. Digital PCR, used in the past also by different terms, is now at the apex of progression and applications in biological sciences owing to developments in chemical sciences, instrumentation, and accessibility of genome sequence data.
  • Highlights

  • Compared to qPCR, the digital PCR is more precise accurate for determining DNA and RNA quantities and mutations therein

  • Digital PCR is used in a variety of nucleic acid analytical and quantification methods.

Keywords

Digital PCR Types of digital PCR Livestock applications Adulterations detection 

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Birbal Singh
    • 1
    Email author
  • Gorakh Mal
    • 1
  • Sanjeev K. Gautam
    • 2
  • Manishi Mukesh
    • 3
  1. 1.ICAR-Indian Veterinary Research Institute, Regional StationPalampurIndia
  2. 2.Department of BiotechnologyKurukshetra UniversityKurukshetraIndia
  3. 3.Department of Animal BiotechnologyICAR-National Bureau of Animal Genetic ResourcesKarnalIndia

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