Reproduction Biotechnology in Cattle

  • Birbal SinghEmail author
  • Gorakh Mal
  • Sanjeev K. Gautam
  • Manishi Mukesh


Cattle are the most important domestic ungulate livestock species used for food- and agriculture-based economic gains worldwide. Several breeds of cattle are domesticated for produce milk, meat, skin, draught power, and manure. Reproduction biotechniques entailing in vitro production of embryos, cryopreservation, sperm sexing, stem cell biology, and genetic engineering to produce value-added products and nuclear transfer cloning are now used in cattle.
  • Highlights

  • Cattle contribute to milk, meat, manure and skin, and draught power

  • Genetic engineering and assisted reproduction techniques have made use of cattle to produce recombinant therapeutics.


Cattle Native cattle breeds Milk and meat Cattle reproduction Cryopreservation Stem cells Transgenic cattle 


  1. Akiyama K, Kobayashi J, Sato Y, Sata R, Ohashi M, Sasaki E, Oda Y, Ogawa Y, Ueda S, Nabenishi H, Matoba S (2010) Calf production from vitrified bovine sexed embryos following in-straw dilution. Anim Sci J 81:461–466PubMedCrossRefGoogle Scholar
  2. Anzalone DA, Palazzese L, Iuso D, Martino G, Loi P (2018) Freeze-dried spermatozoa: an alternative biobanking option for endangered species. Anim Reprod Sci 190:85–93. (Epub 2018 Jan 31)PubMedCrossRefGoogle Scholar
  3. Blondin P, Bousquet D, Twagiramungu H, Barnes F, Sirard MA (2002) Manipulation of follicular development to produce developmentally competent bovine oocytes. Biol Reprod 66(1):38–43PubMedCrossRefGoogle Scholar
  4. Bogliotti YS, Wu J, Vilarino M, Okamura D, Soto DA, Zhong C, Sakurai M, Sampaio RV, Suzuki K, Izpisua Belmonte JC, Ross PJ (2018) Efficient derivation of stable primed pluripotent embryonic stem cells from bovine blastocysts. Proc Natl Acad Sci U S A 115(9):2090–2095. (Epub 2018 Feb 9)CrossRefGoogle Scholar
  5. Bondioli KR, Ellis SB, Pryor JH, Williams MW, Harpold MM (1989) The use of male-specific chromosomal DNA fragments to determine the sex of bovine preimplantation embryos. Theriogenology 31:95–104CrossRefGoogle Scholar
  6. Brackett BG, Bousquet D, Boice ML, Donawick WJ, Evans JF, Dressel MA (1982) Normal development following in vitro fertilization in the cow. Biol Reprod 27(1):147–158PubMedPubMedCentralCrossRefGoogle Scholar
  7. Brophy B, Smolenski G, Wheeler T, Wells D, L’Huillier P, Laible G (2003) Cloned transgenic cattle produce milk with higher levels of beta-casein and kappa-casein. Nat Biotechnol 21(2):157–162 Epub 2003 Jan 27PubMedCrossRefGoogle Scholar
  8. Carlson DF, Lancto CA, Zang B, Kim ES, Walton M, Oldeschulte D, Seabury C, Sonstegard TS, Fahrenkrug SC (2016) Production of hornless dairy cattle from genome-edited cell lines. Nat Biotechnol 34(5):479–481. (No abstract available)PubMedCrossRefGoogle Scholar
  9. Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Ponce de León FA, Robl JM (1998a) Cloned transgenic calves produced from nonquiescent fetal fibroblasts. Science 280(5367):1256–1258PubMedPubMedCentralCrossRefGoogle Scholar
  10. Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Ponce de León FA, Robl JM (1998b) Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells. Nat Biotechnol 16(7):642–646PubMedCrossRefGoogle Scholar
  11. Cibelli JB, Cunniff K, Vrana KE (2006) Embryonic stem cells from parthenotes. Methods Enzymol 418:117–135PubMedCrossRefGoogle Scholar
  12. Cortez JV, Vajta G, Valderrama NM, Portocarrero GS, Quintana JM (2018) High pregnancy and calving rates with a limited number of transferred handmade cloned bovine embryos. Cell Reprogram 20(1):4–8. Scholar
  13. da Silva CG, Martins CF, Cardoso TC, da Cunha ER, Bessler HC, Martins GH, Pivato I, Báo SN (2016) Production of bovine embryos and calves cloned by nuclear transfer using mesenchymal stem cells from amniotic fluid and adipose tissue. Cell Reprogram 18(2):127–136. Scholar
  14. Echelard Y, Williams JL, Destrempes MM, Koster JA, Overton SA, Pollock DP, Rapiejko KT, Behboodi E, Masiello NC, Gavin WG, Pommer J, Van Patten SM, Faber DC, Cibelli JB, Meade HM (2009) Production of recombinant albumin by a herd of cloned transgenic cattle. Transgenic Res 18(3):361–376. (Epub 2008 Nov 22)PubMedCrossRefGoogle Scholar
  15. Enright BP, Taneja M, Schreiber D, Riesen J, Tian XC, Fortune JE, Yang X (2002) Reproductive characteristics of cloned heifers derived from adult somatic cells. Biol Reprod 66(2):291–296PubMedCrossRefGoogle Scholar
  16. Hare WCD, Mitchell D, Betteridge KJ, Eaglesome MD, Randall GCB (1976) Sexing two-week old bovine embryos by chromosomal analysis prior to surgical transfer: preliminary methods and results. Theriogenology 5:243–253CrossRefGoogle Scholar
  17. Herr CM, Reed KC (1989) Micronanipulation of bovine embryos for sex determination. Theriogenology 35:45–54CrossRefGoogle Scholar
  18. Ishino T, Hashimoto M, Amagasa M, Saito N, Dochi O, Kirisawa R, Kitamura H (2018) Establishment of protocol for preparation of gene-edited bovine ear-derived fibroblasts for somatic cell nuclear transplantation. Biomed Res 39(2):95–104. Scholar
  19. Jo HT, Bang JI, Kim SS, Choi BH, Jin JI, Kim HL, Jung IS, Suh TK, Ghanem N, Wang Z, Kong IK (2014) Production of female bovine embryos with sex-sorted sperm using intracytoplasmic sperm injection: efficiency and in vitro developmental competence. Theriogenology 81(5):675–682.e1. (Epub 2013 Nov 21)CrossRefGoogle Scholar
  20. Kaneda M, Watanabe S, Akagi S, Inaba Y, Geshi M, Nagai T (2017) Proper reprogramming of imprinted and non-imprinted genes in cloned cattle gametogenesis. Anim Sci J 88(11):1678–1685. (Epub 2017 Jun 2)PubMedCrossRefGoogle Scholar
  21. Kasinathan P, Wei H, Xiang T, Molina JA, Metzger J, Broek D, Kasinathan S, Faber DC, Allan MF (2015) Acceleration of genetic gain in cattle by reduction of generation interval. Sci Rep 2(5):8674. Scholar
  22. Kubota C, Tian XC, Yang X (2004) Serial bull cloning by somatic cell nuclear transfer. Nat Biotechnol 22(6):693–694 (Epub 2004 May 23)PubMedCrossRefGoogle Scholar
  23. Kubota C, Yamakuchi H, Todoroki J, Mizoshita K, Tabara N, Barber M, Yang X (2000) Six cloned calves produced from adult fibroblast cells after long-term culture. Proc Natl Acad Sci U S A. 97(3):990–995PubMedPubMedCentralCrossRefGoogle Scholar
  24. Lanza RP, Cibelli JB, Faber D, Sweeney RW, Henderson B, Nevala W, West MD, Wettstein PJ (2001) Cloned cattle can be healthy and normal. Science 294(5548):1893–1894 (No abstract available)PubMedCrossRefGoogle Scholar
  25. Kuwayama M, Vajta G, Ieda S, Kato O (2005) Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination. Reprod Biomed Online. 11(5):608–614PubMedCrossRefGoogle Scholar
  26. Matsushita H, Sano A, Wu H, Wang Z, Jiao JA, Kasinathan P, Sullivan EJ, Kuroiwa Y (2015) Species-specific chromosome engineering greatly improves fully human polyclonal antibody production profile in cattle. PLoS One 10(6):e0130699. (eCollection 2015)PubMedPubMedCentralCrossRefGoogle Scholar
  27. Moore SG, Hasler JF (2017) A 100-year review: reproductive technologies in dairy science. J Dairy Sci 100(12):10314–10331. (Review)PubMedCrossRefGoogle Scholar
  28. Morrell JM, Valeanu AS, Lundeheim N, Johannisson A (2018) Sperm quality in frozen beef and dairy bull semen. Acta Vet Scand 60(1):41. Scholar
  29. Nagata MPB, Endo K, Ogata K, Yamanaka K, Egashira J, Katafuchi N, Yamanouchi T, Matsuda H, Goto Y, Sakatani M, Hojo T, Nishizono H, Yotsushima K, Takenouchi N, Hashiyada Y, Yamashita K (2018) Live births from artificial insemination of microfluidic-sorted bovine spermatozoa characterized by trajectories correlated with fertility. Proc Natl Acad Sci U S A 115(14):E3087–E3096. (Epub 2018 Mar 19)CrossRefGoogle Scholar
  30. Pipino C, Mandatori D, Buccella F, Lanuti P, Preziuso A, Castellani F, Grotta L, Di Tomo P, Marchetti S, Di Pietro N, Cichelli A, Pandolfi A, Martino G (2018) Identification and characterization of a stem cell-like population in bovine milk: a potential new source for regenerative medicine in veterinary. Stem Cells Dev. Scholar
  31. Polge C, Rowson LEA (1952) Fertilizing capacity of bull spermatozoa after freezing at −79 °C. Nature 169:626–627PubMedPubMedCentralCrossRefGoogle Scholar
  32. Prather RS, Barnes FL, Sims MM, Robl JM, Eyestone WH, First NL (1987a) Nuclear transplantation in the bovine embryo: assessment of donor nuclei and recipient oocyte. Biol Reprod 37(4):859–866PubMedPubMedCentralCrossRefGoogle Scholar
  33. Prather RS, Spire MF, Schalles RR (1987b) Evaluation of cryopreservation techniques for bovine embryos. Theriogenology 28(2):195–204PubMedCrossRefGoogle Scholar
  34. Richt JA, Kasinathan P, Hamir AN, Castilla J, Sathiyaseelan T, Vargas F, Sathiyaseelan J, Wu H, Matsushita H, Koster J, Kato S, Ishida I, Soto C, Robl JM, Kuroiwa Y (2007) Production of cattle lacking prion protein. Nat Biotechnol 25(1):132–138 Epub 2006 Dec 31PubMedCrossRefGoogle Scholar
  35. Ross PJ, Beyhan Z, Iager AE, Yoon SY, Malcuit C, Schellander K, Fissore RA, Cibelli JB (2008) Parthenogenetic activation of bovine oocytes using bovine and murine phospholipase C zeta. BMC Dev Biol 19(8):16. Scholar
  36. Sano A, Matsushita H, Wu H, Jiao JA, Kasinathan P, Sullivan EJ, Wang Z, Kuroiwa Y (2014) Physiological level production of antigen-specific human immunoglobulin in cloned transchromosomic cattle. PLoS One 8(10):e78119. (eCollection 2013). Erratum in: PLoS One 9(1).
  37. Selvaraju S, Parthipan S, Somashekar L, Binsila BK, Kolte AP, Arangasamy A, Ravindra JP, Krawetz SA (2018) Current status of sperm functional genomics and its diagnostic potential of fertility in bovine(Bos taurus). Syst Biol Reprod Med 1–18. (Epub ahead of print)PubMedCrossRefGoogle Scholar
  38. Sirard MA (2018) 40 years of bovine IVF in the new genomic selection context. Reproduction 156(1):R1–R7. (Epub 2018 Apr 10. Review)PubMedCrossRefGoogle Scholar
  39. Tecirlioglu RT, French AJ, Lewis IM, Vajta G, Korfiatis NA, Hall VJ, Ruddock NT, Cooney MA, Trounson AO (2003) Birth of a cloned calf derived from a vitrified hand-made cloned embryo. Reprod Fertil Dev 15(7–8):361–366PubMedCrossRefGoogle Scholar
  40. Thomas JM, Locke JWC, Vishwanath R, Hall JB, Ellersieck MR, Smith MF, Patterson DJ (2017) Effective use of SexedULTRA™ sex-sorted semen for timed artificial insemination of beef heifers. Theriogenology 98:88–93. Epub 2017 Mar 23PubMedCrossRefGoogle Scholar
  41. Vajta G, Bartels P, Joubert J, de la Rey M, Treadwell R, Callesen H (2004) Production of a healthy calf by somatic cell nuclear transfer without micromanipulators and carbon dioxide incubators using the Handmade Cloning (HMC) and the Submarine Incubation System (SIS). Theriogenology 62(8):1465–1472PubMedCrossRefGoogle Scholar
  42. Vajta G, Holm P, Kuwayama M, Booth PJ, Jacobsen H, Greve T, Callesen H (1998) Open Pulled Straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol Reprod Dev 51(1):53–58CrossRefGoogle Scholar
  43. Vajta G, Lewis IM, Hyttel P, Thouas GA, Trounson AO (2001) Somatic cell cloning without micromanipulators. Cloning 3(2):89–95PubMedCrossRefGoogle Scholar
  44. Vajta G, Maddox-Hyttel P, Skou CT, Tecirlioglu RT, Peura TT, Lai L, Murphy CN, Prather RS, Kragh PM, Callesen H (2005) Highly efficient and reliable chemically assisted enucleation method for handmade cloning in cattle. Reprod Fertil Dev 17(8):791–797PubMedCrossRefGoogle Scholar
  45. Vajta G, Peura TT, Holm P, Páldi A, Greve T, Trounson AO, Callesen H (2000) New method for culture of zona-included or zona-free embryos: the Well of the Well (WOW) system. Mol Reprod Dev 55(3):256–264PubMedCrossRefGoogle Scholar
  46. Vieira AD, Mezzalira A, Barbieri DP, Lehmkuhl RC, Rubin MI, Vajta G (2002) Calves born after open pulled straw vitrification of immature bovine oocytes. Cryobiology 45(1):91–94PubMedCrossRefGoogle Scholar
  47. Wilmut I, Rowson LE (1973) Experiments on the low-temperature preservation of cow embryos. Vet. Rec. 92:686–690PubMedCrossRefGoogle Scholar
  48. Wu X, Song M, Yang X, Liu X, Liu K, Jiao C, Wang J, Bai C, Su G, Liu X, Li G (2016) Establishment of bovine embryonic stem cells after knockdown of CDX2. Sci Rep. 20(6):28343. Scholar
  49. Yadav PS, Kues WA, Herrmann D, Carnwath JW, Niemann H (2005) Bovine ICM derived cells express the Oct4 ortholog. Mol Reprod Dev 72(2):182–190PubMedCrossRefGoogle Scholar
  50. Yadav PS, Singh RK, Singh B (2012) Animal fetal stem cells- potential health applications. Agri Res 1:67–77CrossRefGoogle Scholar
  51. Yamanaka KI, Yamashita K, Khatun H, Wada Y, Tatemoto H, Sakatani M, Takenouchi N, Takahashi M, Watanabe S (2018) Normal DNA methylation status in sperm from a somatic cell cloned bull and their fertilized embryos. Anim Sci J. (Epub ahead of print)PubMedCrossRefGoogle Scholar
  52. Yu M, Muteti C, Ogugo M, Ritchie WA, Raper J, Kemp S (2016) Cloning of the African indigenous cattle breed Kenyan Boran. Anim Genet 47(4):510–511. Scholar
  53. Yuan Y (2018) Capturing bovine pluripotency. Proc Natl Acad Sci U S A 115(9):1962–1963. (Epub 2018 Feb 14. No abstract available)CrossRefGoogle Scholar
  54. Zawada WM, Cibelli JB, Choi PK, Clarkson ED, Golueke PJ, Witta SE, Bell KP, Kane J, Ponce de Leon FA, Jerry DJ, Robl JM, Freed CR, Stice SL (1998) Somatic cell cloned transgenic bovine neurons for transplantation in parkinsonian rats. Nat Med 4(5):569–574PubMedCrossRefGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Birbal Singh
    • 1
    Email author
  • Gorakh Mal
    • 1
  • Sanjeev K. Gautam
    • 2
  • Manishi Mukesh
    • 3
  1. 1.ICAR-Indian Veterinary Research Institute, Regional StationPalampurIndia
  2. 2.Department of BiotechnologyKurukshetra UniversityKurukshetraIndia
  3. 3.Department of Animal BiotechnologyICAR-National Bureau of Animal Genetic ResourcesKarnalIndia

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