Somatic Cell Nuclear Transfer

  • Birbal SinghEmail author
  • Gorakh Mal
  • Sanjeev K. Gautam
  • Manishi Mukesh


In biological system, the term “cloning” has different meanings. Animal cloning, the somatic cell nuclear transfer (SCNT) cloning, became a buzzword when Dolly, the first mammalian species was born from the nuclear transfer of fibroblasts taken from adult sheep. The technique revolutionized the field regenerative medicine, and paved way to engineer stem cells for biomedical applications. At present, a number of animal species including cloned transgenic animals are produced. There are scopes to improve the technique for its wider use for health and welfare of humans.

  • The current nuclear transfer cloning is a more-than-two-decade-old technique that led to concept of direct reprogramming of mature cells.

  • Use of technique is advocated to create animals for experiments and biomedical testing.


SCNT Cloned animals Enucleation Electrofusion Diversity conservation Reprogramming 


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Birbal Singh
    • 1
    Email author
  • Gorakh Mal
    • 1
  • Sanjeev K. Gautam
    • 2
  • Manishi Mukesh
    • 3
  1. 1.ICAR-Indian Veterinary Research Institute, Regional StationPalampurIndia
  2. 2.Department of BiotechnologyKurukshetra UniversityKurukshetraIndia
  3. 3.Department of Animal BiotechnologyICAR-National Bureau of Animal Genetic ResourcesKarnalIndia

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