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Silk Fibroin-Decorin Engineered Biologics to Repair Musculofascial Defects

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Regenerative Medicine and Plastic Surgery

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Abstract

The use of synthetic mesh (e.g., polypropylene [PP] mesh) and biologic (e.g., porcine acellular dermal matrix (PADM)) for the repair of abdominal wall musculofascial has limitations associated with an increased risk of complications and finite properties, respectively. Silk fibroin (SF)-based scaffolds are fabricated with decorin blends (SFD) to create a matrix platform with the desired structure-property function to be translated to the clinic to address patient-specific needs. PADM and SFD composites with SFD or PADM facing the peritoneal cavity were tested in an in vivo incisional ventral hernia repair model using female Hartley guinea pigs. At week 4, gross observation of adhesion grade and strength showed that the SFD, PADM, and composites resulted in fewer and weaker adhesions than did PP mesh (p < 0.05). Mechanical properties at the musculofascial-implant interface in the SFD group were similar to those of the native abdominal wall. SFD group exhibited homogeneous three-dimensional cell infiltration, vascularization, and tissue remodeling in implants. In conclusion, SFD promotes musculofascial regeneration.

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Acknowledgements

This study was supported by National Institutes of Health (NIH) and National Institute on Aging (NIA) via NIH/NIA grant R01AG034658. We thank Dr. Samuel M. Hudson (North Carolina State University) for the donation of raw silk and Carmen N. Rios and Victor L. Lam for technical assistance. In addition, we thank the High-Resolution Electron Microscopy Facility (HREMF; Cancer Center Core Grant CA16672) for scanning electron microscopy imaging.

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Authors and Affiliations

  1. Department of Plastic Surgery, Unit 602, The University of Texas MD Anderson Cancer Center, Houston, TX, USA

    Lina W. Dunne, Nadja Falk, Justin Hubenak, Tejaswi S. Iyyanki, Vishal Gupta, Qixu Zhang, Charles E. Butler & Anshu B. Mathur

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  1. Lina W. Dunne
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  2. Nadja Falk
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  3. Justin Hubenak
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  4. Tejaswi S. Iyyanki
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  5. Vishal Gupta
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  6. Qixu Zhang
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  7. Charles E. Butler
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  8. Anshu B. Mathur
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Corresponding author

Correspondence to Anshu B. Mathur .

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Editors and Affiliations

  1. Department for Plastic Surgery and Hand Surgery, Division of Experimental Plastic Surgery, Technical University of Munich, Munich, Germany

    Dominik Duscher

  2. Private Practice, Tustin, CA, USA

    Melvin A. Shiffman

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Dunne, L.W. et al. (2019). Silk Fibroin-Decorin Engineered Biologics to Repair Musculofascial Defects. In: Duscher, D., Shiffman, M.A. (eds) Regenerative Medicine and Plastic Surgery. Springer, Cham. https://doi.org/10.1007/978-3-030-19962-3_23

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