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Dispensers for Entomovectoring: For Every Bee a Different Type?

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Entomovectoring for Precision Biocontrol and Enhanced Pollination of Crops

Abstract

To load pollinators with supplemental pollen or a biocontrol organism (BCO) in an efficient way, the use of a suitable dispenser is crucial. Over the past years, multiple studies have been conducted to optimize dispensers for honey bees, bumble bees and mason bees and test their potential for application with the entomovector technology. Research on dispensers for honey bees started by testing simple one-way dispensers, previously used to load pollinators with pollen, but they soon proved to be inefficient in loading the bees with a BCO. More efficient two-way dispensers, where entrance and exit ways are separated, were developed and improved, ultimately leading to an efficient model that was suitable for commercial use, the BeeTreat dispenser. As behavioral differences between honey bees and bumble bees proved to be a problem in attempts to load bumble bees using the honey bee dispensers, dedicated models were developed to achieve a sufficient loading and use bumble bees as a vector for BCO delivery. Today, two commercial dispensers are available for bumble bees. While the development of dispensers for mason bees is still at an earlier stage, progress on optimizing the dispenser model shows promising forthcoming results. Future effort should be devoted, but not limited to, increasing our knowledge on the pollination and entomovectoring potential of solitary bee species, like those of the genus of Osmia. Alongside with the continued improvement of the dispenser models, the main focus of the research should shift towards improving the powder formulation which can be loaded in the dispenser to increase the efficiency of the entomovector technology.

Bettina Maccagnani - Scientific advisor for the Automobili Lamborghini project “Environmental Biomonitoring with Honey Bees: Science and Education”

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References

  • Albano S, Chagnon M, De Oliveira D, Houle E, Thibodeau P, Mexia A (2009) Effectiveness of Apis mellifera and Bombus impatiens as dispensers of the Rootshield® biofungicide (Trichoderma harzianum, strain T-22) in a strawberry crop. Hell Plant Prot J 2:57–66

    Google Scholar 

  • Antles L (1953) New methods in orchard pollination. Am Bee J 93:102–103

    Google Scholar 

  • Biddinger D, Ngugi H, Frazie RJ, Frazier M, Leslie T, Donovall L (2010) Development of the mason bee, Osmia cornifrons, as an alternative pollinator to honey bees and as a targeted delivery system for biological control agents in the management of fire blight. Penn Fruit News:35–44

    Google Scholar 

  • Bilu A, Dag A, Elad Y, Shafir S (2004) Honey bee dispersal of biocontrol agents: an evaluation of dispensing devices. Biocontrol Sci Tech 14:607–617

    Article  Google Scholar 

  • Dag A, Weinbaum SA, Thorp RW, Eisikowitch D (2000) Pollen dispensers (inserts) increase fruit set and yield in almonds under some commercial conditions. J Apic Res 39:117–123

    Article  Google Scholar 

  • Dedej S, Delaphane KS, Scherm H (2004) Effectiveness of honey bees in delivering the biocontrol agent Bacillus subtilis to blueberry flowers to suppress mummy berry disease. Biol Control 31:422–427

    Article  Google Scholar 

  • Gross HR, Hamm JJ, Carpenter JE (1994) Desing and application of a hive-mounted device that uses honey-bees (Hymenoptera, Apidae) to disseminate Heliothis nuclear Polyhedrosis virus. Environ Entomol 23:492–501

    Article  Google Scholar 

  • Hokkanen HM, Menzler-Hokkanen I (2009) Successful use of honey bees for grey mould biocontrol on strawberries and raspberries in Finland. Apidologie 40:659

    Google Scholar 

  • Hokkanen HMT, Menzler-Hokkanen I, Mustalahti A-M (2012) Honey bees (Apis mellifera) for precision biocontrol of grey mould (Botrytis cinerea) with Gliocladium catenulatum on strawberries and raspberries in Finland. Arthropod-plant interactions (submitted)

    Google Scholar 

  • Hokkanen HMT, Menzler-Hokkanen I, Levy M (2014) BICOPOLL Final Report for Finland. BICOPOLL Final Meeting, 22–26 September 2014, Åland Islands, Finland

    Google Scholar 

  • Johnson KB, Stockwell VO, Burgett DM, Sugar D, Loper JE (1993) Dispersal of Erwinia amylovora and Pseudomonas fluorescens by honey bees from hives to apple and pear blossoms. Phytopathology 83:478–484

    Article  Google Scholar 

  • Jyoti JL, Brewer GJ (1999) Honey bees (Hymenoptera: Apidae) as vectors of Bacillus thuringiensis for control of banded sunflower moth (Lepidoptera: Tortricidae). Environ Entomol 28:1172–1176

    Article  Google Scholar 

  • Kovach J, Petzoldt R, Harman GE (2000) Use of honey bees and bumble bees to disseminate Trichoderma harzianum 1295–22 to strawberries for Botrytis control. Biol Control 18:235–242

    Article  Google Scholar 

  • Legge A (1976) Hive inserts and pollen dispensers for tree fruits. Bee World 57:159–167

    Article  Google Scholar 

  • Maccagnani B, Ladurner E, Santi F, Burgio G (2003) Osmia cornuta (Hymenoptera, Megachilidae) as a pollinator of pear (Pyrus communis): fruit-and seed-set. Apidologie 34:207–216

    Article  Google Scholar 

  • Maccagnani B, Mocioni M, Ladurner E, Gullino ML, Maini S (2005) Investigation of hive-mounted devices for the dissemination of microbiological preparations by Bombus terrestris. Bull Insectol 58:3–8

    Google Scholar 

  • Maccagnani B, Bazzi C, Biondi E, Tesoriero D, Maini S (2006) Potential of Osmia cornuta as a carrier of antagonist bacteria in biological control of fire blight: a comparison with Apis mellifera. In: Bazzi C, Mazzucchi U (eds) Proceedings of the Xth international workshop on fire blight. Acta Horticulturae. International Society Horticultural Science, Leuven, pp 379–386

    Google Scholar 

  • Maccagnani B, Giacomello F, Fanti M, Gobbin D, Maini S, Angeli G (2008) Apis mellifera and Osmia cornuta as carriers for the secondary spread of Bacillus subtilis on apple flowers. BioControl 54:123–133

    Article  Google Scholar 

  • Mayer DF, Johansen CA (1988) WSU research examines bee hive pollen dispeners. Good Fruit Grow 39:32–33

    Google Scholar 

  • Mommaerts V, Smagghe G (2011) Entomovectoring in plant protection. Arthropod Plant Interact 5:81–95

    Article  Google Scholar 

  • Mommaerts V, Put K, Vandeven J, Jans K, Sterk G, Hoffmann L, Smagghe G (2010) Development of a new dispenser for microbiological control agents and evaluation of dissemination by bumble bees in greenhouse strawberries. Pest Manag Sci 66:1199–1207

    Article  CAS  Google Scholar 

  • Mommaerts V, Put K, Vandeven J, Smagghe G (2012) Miniature-dispenser-based bioassay to evaluate the compatibility of powder formulations used in an entomovectoring approach. Pest Manag Sci 68:922–927

    Article  CAS  Google Scholar 

  • Monzon VH, Bosch J, Retana J (2004) Foraging behavior and pollinating effectiveness of Osmia cornuta (Hymenoptera : Megachilidae) and Apis mellifera (Hymenoptera: Apidae) on “Comice” pear. Apidologie 35:575–585

    Article  Google Scholar 

  • Peng G, Sutton JC, Kevan PG (1992) Effectivenes of honey bees for applying the biocontrol agent Gliocladiu roseum to strawberry flowers to suppress Botrytis cinerea. Can J Plant Pathol Revue Can Phytopathol 14:117–129

    Article  Google Scholar 

  • Scherm H, Ngugi HK, Savelle AT, Edwards JR (2004) Biological control of infection of blueberry flowers caused by Monilinia vaccinii corymbosi. Biol Control 29:199–206

    Article  Google Scholar 

  • Smagghe G, Mommaerts V, Hokkanen H, Menzler-Hokkanen I (2012) Multitrophic interactions: he entomovector technology. In: Smagghe G, Diaz I (eds) Arthropod-plant interactions: novel insights and approaches for IPM. Springer Netherlands, Dordrecht, pp 127–157

    Chapter  Google Scholar 

  • Thomson SV, Hansen DR, Flint KM (1992) Dissemination of bacteria antagonistic to Erwinia-amylovora by honey bees. Plant Dis 76:1052–1056

    Article  Google Scholar 

  • Vicens N, Bosch J (2000) Pollinating efficacy of Osmia cornuta and Apis mellifera (Hymenoptera: Megachilidae, Apidae) on ‘red Delicious’ apple. Environ Entomol 29:235–240

    Article  Google Scholar 

  • Yu H, Sutton J (1997) Effectiveness of bumble bees and honey bees for delivering inoculum of Gliocladium roseum to raspberry flowers to control Botrytis cinerea. Biol Control 10:113–122

    Article  Google Scholar 

Download references

Acknowledgements

Financial support to BICOPOLL from the EU ERA-NET project CORE Organic 2, to the project “Entomovectoring in plant protection” from NordForsk and to the project BICOPOLL-NET from NordForsk, are gratefully acknowledged. The authors wish to thank Roberto Ferrari and Marco Pozzati, for their precious help in the research during the study for the development of entomovectoring technique using Osmia cornuta. The authors also acknowledge the Special Research Fund of the Ghent University, the Flemish Agency for Innovation by Science and Technology (IWT-Flanders, Brussels), and the Fund for Scientific Research (FWO-Flanders, Brussels).

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Maccagnani, B., Pisman, M., Smagghe, G. (2020). Dispensers for Entomovectoring: For Every Bee a Different Type?. In: Smagghe, G., Boecking, O., Maccagnani, B., Mänd, M., Kevan, P. (eds) Entomovectoring for Precision Biocontrol and Enhanced Pollination of Crops. Springer, Cham. https://doi.org/10.1007/978-3-030-18917-4_6

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