Abstract
The olive seed is a promising by product generated in the olive oil related industries, with increasing interest because of its nutritional value and potential nutraceutical properties. Knowledge concerning the antioxidant capacity of this new alimentary material is scarce. Moreover, oxidative homeostasis and signaling involved physiological processes such as development, dormancy and germination in the olive seed are also unknown. Glutathione (one of the most abundant antioxidants in plant cells), is crucial for seeds physiology, and for defense and detoxification mechanisms. The availability of glutathione in its reduced (GSH) and oxidized (GSSG) forms, the ratio of both forms (GSH/GSSG), and their concurrence in other numerous metabolic pathways is tightly regulated by numerous enzymes. Prominent among these enzymes is glutathione reductase (GR), which has been considered essential for seedling growth and development. The present work aims to increase the knowledge about the functional insights of GR in olive seeds. Searching in the olive transcriptome, at least 19 GR homologues (10 from seed and 9 from vegetative tissue) were identified and retrieved. An in silico analysis was carried out, which included phylogeny, 3-D modelling of the N-terminus, and the prediction of cellular localization and post-translational modifications (PTM) for these gene products. The high variability of forms detected for this enzyme in olive seeds and their susceptibility to numerous PTMs suggest a relevant role for this enzyme in redox metabolism and signalling events.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Tsimidou, M.Z.: Virgin olive oil (VOO) and other olive tree products as sources of α-tocopherol. Updating and perspective. In: Catala, A. (ed.) Tocopherol Sources, Uses and Health Benefits, pp. 1–21. Nova Science Publisher, New York (2012)
Balanza, R., Garcia-Lorda, P., Perez-Rodrigo, C., Aranceta, J., Bonet, M.B., Salas-Salvado, J.: Trends in food availability determined by the Food and Agriculture Organization’s food balance sheets in Mediterranean Europe in comparison with other European areas. Public Health Nutr. 10, 168–176 (2007)
Boskou, D.G.: Olives and Olive Oil Bioactive Constituents, 1st ed. AOCS, Urbana (2015)
Blekas, G., Vassilakis, C., Harizanis, C., Tsimidou, M., Boskou, D.G.: Biophenols in table olives. J. Agric. Food Chem. 50, 3688–3692 (2002)
Luaces, P., Pérez, A.G., Sanz, C.: Role of olive seed in the biogenesis of virgin olive oil aroma. J. Agric. Food Chem. 51, 4741–4745 (2003)
Luaces, P., Romero, C., Gutierrez, F., Sanz, C., Pérez, A.G.: Contribution of olive seed to the phenolic profile and related quality parameters of virgin olive oil. J. Sci. Food Agric. 87, 2721–2727 (2007)
Leprince, O., Hendry, G.A.F., Atherton, N.M., Walters-Vertucci, C.: Free radicals and metabolism associated with the acquisition and loss of desiccation tolerance in developing seeds. Biochem. Soc. Trans. 24, 451–455 (1996)
Bailly, C.: Active oxygen species and antioxidants in seed biology. Seed Sci. Res. 14, 93–107 (2004)
Kocsy, G., Galiba, G., Brunold, C.: Role of glutathione in adaptation and signaling during chilling and cold acclimation in plants. Physiol. Plantarium 113, 158–164 (2001)
Schopfer, P., Plachy, C., Frahry, G.: Release of reactive oxygen intermediates (superoxide radicals, hydrogen peroxide, and hydroxyl radicals) and peroxidase in geminating radish seeds controlled by light, gibberellin, and abscisic acid. Plant Physiol. 125, 1591–1602 (2001)
Gechev, T.S., Van Breusegem, F., Stone, J.M., Denev, I., Laloi, C.: Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. BioEssays 28, 1091–1101 (2006)
Starke, D.W., Chock, P.B., Mieyal, J.J.: Glutathione-thiyl radical scavenging and transferase properties of human glutaredoxin, (thioltransferase): potential role in redox signal transduction. J. Biol. Chem. 278, 14607–14613 (2003)
Meyer, A.J., May, M.J., Fricker, M.: Quantitative in vivo measurement of glutathione in Arabidopsis cells. Plant J. 27, 67–78 (2001)
Mullineaux, P., Creissen, G.: Glutathione reductase: regulation and role in oxidative stress. In: Scandalios, J.G. (ed.) Oxidative Stress and the Molecular Biology of Antioxidant Defense, pp. 667–713. Cold Spring Harbor, Plainview (1997)
Bailly, C., Benamar, A., Corbineau, F., Come, D.: Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated ageing. Physiol. Plantarium 97, 104–110 (1996)
Hendry, G.A., et al.: Free radical processes and loss of seed viability during desiccation in the recalcitrant species Quercus robur L. New Phytol. 122, 273–279 (1992)
Carmona, R., et al.: ReprOlive: a database with linked data for the olive tree (Olea europaea L.) reproductive transcriptome. Front. Plant Sci. 6, 625 (2015)
McWilliam, H., et al.: Analysis tool web services from the EMBL-EBI. Nucleic Acids Res. 41, W597–W600 (2013)
Gouy, M., Guindon, S., Gascuel, O.: SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building. Mol. Biol. Evol. 27, 221–224 (2010)
Darriba, D., Taboada, G.L., Doallo, R., Posada, D.: jModelTest 2: more models, new heuristics and parallel computing. Nat. Methods 9, 772 (2012)
Xue, Y., et al.: GPS-SNO: computational prediction of protein S-nitrosylation sites with a modified GPS algorithm. PLoS One 5, e11290 (2010)
Martinez, A., et al.: Extent of N-terminal modifications in cytosolic proteins from eukaryotes. Proteomics 8, 2809–2831 (2008)
Kelley, L.A., Sternberg, M.J.: Protein structure prediction on the web: a case study using the Phyre server. Nat. Protoc. 4, 363–371 (2009)
Cruz, F., et al.: Genome sequence of the olive tree Olea europaea. Gigascience 5, 29 (2016)
Creissen, G., Edwards, E.A., Enard, C., Wellburn, A.R., Mullineaux, P.: Molecular characterization of glutathione reductase cDNAs from pea (Pisum sativum L.). Plant J. 2(1), 129–131 (1992)
Traverso, J.A., Meinnel, T., Giglione, C.: Expanded impact of protein N-myristoylation in plants. Plant Sig. Behav. 3(7), 501–502 (2008)
Traverso, J.A., et al.: Roles of N-terminal fatty acid acylations in membrane compartment partitioning: Arabidosis h-Type thioredoxins as a case study. Plant Cell 25, 1056–1077 (2013)
Bai, X., et al.: Nitric oxide desiccation tolerance of recalcitrant Antiaris toxicaria seeds via protein S-nitrosylation and carbonylation. PLoS One 6(6), e20174 (2011)
García-Quirós, E., Carmona, R., Zafra, A., Gonzalo Claros, M., Alché, J.D.: Identification and in silico analysis of glutathione reductase transcripts expressed in olive (Olea europaea L.) Pollen and Pistil. In: Rojas, I., Ortuño, F. (eds.) IWBBIO 2017. LNCS, vol. 10209, pp. 185–195. Springer, Cham (2017). https://doi.org/10.1007/978-3-319-56154-7_18
Dym, O., Eisenberg, D.: Sequence-structure analysis of FAD-containing proteins. Protein Sci. 10, 1712–1728 (2001)
Acknowledgments
This work was supported by ERDF-cofunded projects BFU2016-77243-P and RTC-2017-6654-2. EGQ thanks MINECO for FPI grant.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Ethics declarations
The authors confirm that this article content has no conflicts of interest.
Rights and permissions
Copyright information
© 2019 Springer Nature Switzerland AG
About this paper
Cite this paper
Lima-Cabello, E. et al. (2019). Structural and Functional Features of Glutathione Reductase Transcripts from Olive (Olea europaea L.) Seeds. In: Rojas, I., Valenzuela, O., Rojas, F., Ortuño, F. (eds) Bioinformatics and Biomedical Engineering. IWBBIO 2019. Lecture Notes in Computer Science(), vol 11465. Springer, Cham. https://doi.org/10.1007/978-3-030-17938-0_17
Download citation
DOI: https://doi.org/10.1007/978-3-030-17938-0_17
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-17937-3
Online ISBN: 978-3-030-17938-0
eBook Packages: Computer ScienceComputer Science (R0)