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Combinatorial Electrophoresis and Mass Spectrometry-Based Proteomics in Breast Milk for Breast Cancer Biomarker Discovery

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Advancements of Mass Spectrometry in Biomedical Research

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1140))

Abstract

Innovations in approaches for early detection and individual risk assessment of different cancers, including breast cancer (BC), are needed to reduce cancer morbidity and associated mortality. The assessment of potential cancer biomarkers in accessible bodily fluids provides a novel approach to identify the risk and/or onset of cancer. Biomarkers are biomolecules, such as proteins, that are indicative of an abnormality or a disease. Human milk is vastly underutilized biospecimen that offers the opportunity to investigate potential protein BC-biomarkers in young, reproductively active women. As a first step, we have examined the entire protein pattern in human milk samples from breastfeeding mothers with cancer, who were diagnosed either before or after milk donation, and from women without cancer, using mass spectrometry (MS)-based proteomics.

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Abbreviations

1D:

One-dimensional

2D:

Two-dimensional

BC:

Breast cancer

DDA:

Data dependent acquisition

DDT:

Dithiothreitol

IAA:

Iodoacetamide

MS:

Mass spectrometry

MS/MS:

Tandem mass spectrometry

nanoLC-MS/MS:

Nano-liquid chromatography tandem mass spectrometry

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Acknowledgements

We thank the members of our labs for the pleasant working environment.

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Authors and Affiliations

  1. Biochemistry & Proteomics Group, Department of Chemistry & Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Roshanak Aslebagh, Devika Channaveerappa & Costel C. Darie

  2. Department of Veterinary & Animal Sciences, University of Massachusetts, Amherst, MA, USA

    Brian T. Pentecost & Kathleen F. Arcaro

Authors
  1. Roshanak Aslebagh
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  2. Devika Channaveerappa
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  3. Brian T. Pentecost
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  4. Kathleen F. Arcaro
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  5. Costel C. Darie
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Corresponding author

Correspondence to Kathleen F. Arcaro .

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Editors and Affiliations

  1. Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Alisa G. Woods

  2. Biochemistry and Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY, USA

    Costel C. Darie

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Aslebagh, R., Channaveerappa, D., Pentecost, B.T., Arcaro, K.F., Darie, C.C. (2019). Combinatorial Electrophoresis and Mass Spectrometry-Based Proteomics in Breast Milk for Breast Cancer Biomarker Discovery. In: Woods, A., Darie, C. (eds) Advancements of Mass Spectrometry in Biomedical Research. Advances in Experimental Medicine and Biology, vol 1140. Springer, Cham. https://doi.org/10.1007/978-3-030-15950-4_26

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